Abstract: A dental floss holder for holding a length of dental floss under tension comprises an elongated handle and a bifurcated head at one end of the handle, the bifurcated head having a pair of opposed spaced-apart resilient arms defining a gap between the terminal ends thereof. Each terminal end of the arms is provided with an outwardly extending flange and a floss receiving groove, the groove at one terminal end being aligned with the groove at the other terminal end such that a length of the floss bridges the gap. Each groove has a bottom defined by a rounded convex surface for frictionless engagement with the floss to permit the floss to freely slide thereon as the arms inwardly yield towards one another upon the application of tension to the floss. The flange of each arm includes a pair of opposed flange portions defining, as an extension of the groove, a guide opening therebetween for guiding the floss and maintaining same positioned on the bottom surface at each terminal end.