Abstract: A diffractometric sensor (1), comprises:—a substrate (3);—two interdigitated affinity gratings (2), a first affinity grating (20) comprising first unit cells (200) with affinity elements (201) and a second affinity grating (21) comprising second unit cells (210) with affinity elements (211), wherein the first unit cells (200) and second unit cells (210) are configured and arranged such that coherent light of a predetermined wavelength generated at a predetermined beam generation location (40) and diffracted by target molecules (204, 214)) bound to the affinity elements (201, 211) constructively interferes at a predetermined detection location (50) with an inverse phase, and wherein the first and second affinity gratings (20, 21) are balanced to generate a bias signal at the predetermined detection location (50) that corresponds to a difference (Am) in the scattering mass of the first and second affinity gratings (20, 21) which is in the range of 0.001 pg/mm2 to 30000 pg/mm2.

Abstract: Disclosed herein is an artificial transmembrane protein for use in a biomolecular detection device for detecting intracellular or intravesicular biomolecular interactions, the artificial transmembrane protein having an extracellular or extravesicular binder structure, a hydrophobic transmembrane domain, and an intracellular or intravesicular domain with an intracellular or intravesicular receptor structure, wherein the receptor structure is configured to interact with an intracellular or intravesicular component of the biomolecular interaction to be detected and wherein the extracellular or extravesicular binder structure is configured to bind to membrane recognition elements arranged along a plurality of predetermined lines of the biomolecular detection device.

Abstract: Disclosed herein is a biomolecular detection device (1) for analyzing a cell, vesicle or a cellular or vesicular component, comprising an evanescent illuminator with an optical coupling unit configured for generating an evanescent field from coherent light (L) with a predefined wavelength on a first surface of the evanescent illuminator. The first surface of evanescent illuminator comprises a template nanopattern (5), containing a coherent arrangement of a plurality of predetermined lines along which membrane recognition elements for a binder structure (82) of a transmembrane protein (81), preferably a laterally diffusible transmembrane protein, of the cell, vesicle or the cellular or vesicular component (8) are arranged.