Abstract: The present invention relates to a polypeptide comprising an antigenic fragment having a sequence of at least seven amino acids from a hydrophilic domain of an oil body-associated protein, wherein the polypeptide comprises a truncated hydrophobic domain of the oil body-associated protein or does not comprise such a domain, and wherein the oil body-associated protein is selected from the group comprising oleosin, caleosin and steroleosin, to a pharmaceutical composition or a vaccine comprising the polypeptide, to a complex comprising the polypeptide and an antibody bound thereto, to a medical or diagnostic device comprising the polypeptide, to a nucleic acid encoding the polypeptide or a cell comprising said nucleic acid, to a test kit comprising the polypeptide, and to a method for detecting an antibody which binds to the polypeptide.

Abstract: The present invention relates generally to a method of purifying Factor VII and/or Factor VIIa from a solution containing either or both proteins, the method comprising: diluting the solution containing Factor VII and/or Factor VIIa in a loading buffer; adding the diluted solution from step (i) to a multi-modal anion exchange resinunder conditions selected such that Factor VII and/or Factor VIIa is bound to the resin; optionally washing the resin with a wash buffer; adding an elution buffer to the resin under conditions selected such that Factor VII and/or Factor VIIa is eluted from the resin; and recovering the eluted recombinant Factor VII and/or Factor VIIa; wherein the loading buffer, the optional wash buffer and the elution buffer each comprise about 35 mM or less of calcium ions.