Abstract: An intraoperative ultrasound imaging system and method capable of using ultrasound imaging to safely place a surgical access instrument (e.g. guide wire, dilator, cannula, etc.) through a tissue (e.g., muscle, fat, brain, liver, lung, etc.) without damaging nearby neurovascular structure is described herein. The intraoperative ultrasound system includes an ultrasound probe assembly configured for emitting and receiving ultrasound waves and a computer system including a processor and a display unit. Once the probe is in position, ultrasound imaging is performed wherein the computer receives RF data from the probe and causes a B-mode image of the visible anatomical structures (e.g. muscle, bone, etc.) to be displayed on the display unit.