Abstract: Materials and methods are provided for making soybean varieties that have altered oil composition as a result of one or more mutation modulating the expression of a SACPD-C gene, a FATB-1A gene, or both the SACPD-C and FATB-1A genes. For example, a soybean plant, plant part, or plant cell producing an oil that has increased saturated fatty acid content as compared to oil produced from a corresponding soybean plant, plant part, or plant cell lacking the one or more mutations is provided.

Abstract: TALEN compositions and methods of use are disclosed, which include using multiplexing compositions to create a targeted mutation in several genes at once, such as the FAD3 A/B/C genes, compositions to create a targeted mutation in a single gene, such as a gene encoding a FAD2 protein, and combinations thereof. The compositions and methods can provide gene-edited plants, plant parts, and plant cells that have improved characteristics compared to the corresponding unaltered plants, plant parts, or plant cells. For example, soybean plants, plant parts and plant cells that are capable of producing a seed with an oil having comparatively higher levels of oleic acid and lower levels of linoleic and linolenic acid than a corresponding seed lacking the targeted mutation are also provided.

Abstract: Gene-edited soybean plants, plant parts, and plant cells that have a reduced isoflavone content compared to the corresponding unaltered soybean plant, plant part, or plant cell and methods of their production are disclosed. For example, introducing one or more mutations in the coding region of Myb29 gene, the MYBCORE binding motif of the IFS2 promoter, the MYBCORE binding motif of the IFS1 promoter, the coding region of the IFS2 gene, the coding region of the IFS1 gene, or a combination thereof is used to produce the gene-edited plant, plant part or plant cell via targeted genome modification.

Abstract: Materials and methods are provided for making soybean varieties that have altered oil composition as a result of mutations in the FAD3A, FAD3B, and FAD3C genes.

Abstract: Materials and methods are provided for making soybean varieties that have altered oil composition as a result of mutations in the FAD3A, FAD3B, and FAD3C genes.

Abstract: Materials and methods are provided for making soybean varieties that have altered oil composition as a result of mutations in the FAD3A, FAD3B, and FAD3C genes.