Patents by Inventor Zaiguo Li
Zaiguo Li has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11939350Abstract: Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.Type: GrantFiled: November 19, 2020Date of Patent: March 26, 2024Assignee: Enzo Life Sciences, Inc.Inventors: Zaiguo Li, Praveen Pande
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Publication number: 20210079034Abstract: Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.Type: ApplicationFiled: November 19, 2020Publication date: March 18, 2021Applicant: Enzo Life Sciences, Inc.Inventors: Zaiguo Li, Praveen Pande
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Publication number: 20210079225Abstract: The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties.Type: ApplicationFiled: November 2, 2020Publication date: March 18, 2021Applicant: Enzo Biochem, Inc.Inventors: Zaiguo Li, Praveen Pande, Natarajan Raju
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Patent number: 10875886Abstract: Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.Type: GrantFiled: September 11, 2018Date of Patent: December 29, 2020Assignee: Enzo Life Sciences, Inc.Inventors: Zaiguo Li, Praveen Pande
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Patent number: 10865309Abstract: The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties.Type: GrantFiled: October 6, 2018Date of Patent: December 15, 2020Assignee: Enzo Biochem, Inc.Inventors: Zaiguo Li, Praveen Pande, Natarajan Raju
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Patent number: 10683257Abstract: The invention provides methods for synthesizing the compound (2R,3S,4E)-N-methyl-5-(4?-pentylphenyl)-2-aminopent-4-ene-1,3-diol, also known as SK1-I, and intermediate compounds used in its synthesis.Type: GrantFiled: May 13, 2019Date of Patent: June 16, 2020Assignee: Enzo Biochem, Inc.Inventors: Zaiguo Li, Natarajan Raju, Praveen Pande
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Publication number: 20200131113Abstract: The invention provides methods for synthesizing the compound (2R,3S,4E)-N-methyl-5-(4?-pentylphenyl)-2-aminopent-4-ene-1,3-diol, also known as SK1-I, and intermediate compounds used in its synthesis.Type: ApplicationFiled: May 13, 2019Publication date: April 30, 2020Applicant: Enzo Biochem, Inc.Inventors: Zaiguo Li, Natarajan Raju, Praveen Pande
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Publication number: 20190256453Abstract: The invention provides methods for synthesizing the compound (2R,3S,4E)-N-methyl-5-(4?-pentylphenyl)-2-aminopent-4-ene-1,3-diol, also known as SK1-I, and intermediate compounds used in its synthesis.Type: ApplicationFiled: October 24, 2018Publication date: August 22, 2019Applicant: Enzo Biochem, Inc.Inventors: Zaiguo Li, Natarajan Raju, Praveen Pande
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Patent number: 10364211Abstract: The invention provides methods for synthesizing the compound (2R,3S,4E)-N-methyl-5-(4?-pentylphenyl)-2-aminopent-4-ene-1,3-diol, also known as SK1-I, and intermediate compounds used in its synthesis.Type: GrantFiled: October 24, 2018Date of Patent: July 30, 2019Assignee: Enzo Biochem, Inc.Inventors: Zaiguo Li, Natarajan Raju, Praveen Pande
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Publication number: 20190062560Abstract: The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties.Type: ApplicationFiled: October 6, 2018Publication date: February 28, 2019Applicant: Enzo Biochem, Inc.Inventors: Zaiguo Li, Praveen Pande, Natarajan Raju
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Publication number: 20190002491Abstract: Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.Type: ApplicationFiled: September 11, 2018Publication date: January 3, 2019Applicant: ENZO LIFE SCIENCES, INC. C/O ENZO BIOCHEM, INC.Inventors: Zaiguo Li, Praveen Pande
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Patent number: 10131789Abstract: The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties.Type: GrantFiled: March 22, 2018Date of Patent: November 20, 2018Assignee: Enzo Biochem, Inc.Inventors: Zaiguo Li, Praveen Pande, Natarajan Raju
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Patent number: 10106573Abstract: Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.Type: GrantFiled: July 8, 2016Date of Patent: October 23, 2018Assignee: Enzo Life Sciences, Inc.Inventors: Zaiguo Li, Praveen Pande
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Publication number: 20180215920Abstract: The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties.Type: ApplicationFiled: March 22, 2018Publication date: August 2, 2018Applicant: ENZO BIOCHEM, INC.Inventors: ZAIGUO LI, PRAVEEN PANDE, NATARAJAN RAJU
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Patent number: 9957393Abstract: The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties.Type: GrantFiled: March 30, 2015Date of Patent: May 1, 2018Assignee: Enzo Biochem, Inc.Inventors: Zaiguo Li, Praveen Pande, Natarajan Raju
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Patent number: 9951227Abstract: The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties.Type: GrantFiled: September 7, 2017Date of Patent: April 24, 2018Assignee: Enzo Biochem, Inc.Inventors: Zaiguo Li, Praveen Pande, Natarajan Raju
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Publication number: 20170369954Abstract: The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties.Type: ApplicationFiled: September 7, 2017Publication date: December 28, 2017Applicant: Enzo Biochem, Inc.Inventors: Zaiguo Li, Praveen Pande, Natarajan Raju
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Publication number: 20160311846Abstract: Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.Type: ApplicationFiled: July 8, 2016Publication date: October 27, 2016Applicant: ENZO LIFE SCIENCES, INC. C/O ENZO BIOCHEM, INC.Inventors: Zaiguo Li, Praveen Pande
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Publication number: 20160289779Abstract: The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties.Type: ApplicationFiled: March 30, 2015Publication date: October 6, 2016Applicant: Enzo Biochem, Inc.Inventors: ZAIGUO LI, PRAVEEN PANDE, NATARAJAN RAJU
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Patent number: 9416153Abstract: Provided are various compounds comprising the formula Also provided are fluorescent dyes comprising the above compound. Additionally, a fluorescence energy transfer system is provided that comprises the above-described fluorescent dye and a second dye, wherein the second dye is capable of energy transfer with the fluorescent dye. Further provided is a kit for labeling a target molecule, where the kit comprises the above-described fluorescent dye with additional reagents useful for labeling the target molecule. Additionally provided is a target molecule labeled with the above-described fluorescent dye. Methods of labeling a target molecule with the above-described fluorescent dye are also provided.Type: GrantFiled: October 11, 2011Date of Patent: August 16, 2016Assignee: Enzo Life Sciences, Inc.Inventors: Zaiguo Li, Praveen Pande