Abstract: The present invention relates to a MAD7-type nuclease, which has been engineered to recognize a PAM selected from TYCV, TATV or TTCN. The invention provides sequences encoding or representing a MAD7-type nuclease carrying certain mutations compared to the sequence of a MAD7 nuclease. The invention also provides a genome engineering system, an expression construct and a kit comprising a MAD7-type nuclease according to the invention. Moreover, the invention relates to a method for the targeted modification of at least one genomic target sequence in a cell, which comprises introducing the MAD7-type nuclease according to the invention into the cell. The invention also provides a cell and an organism obtained by a method according to the invention.

Abstract: The present invention relates to novel methods for discovering traits and generating cellular systems having improved phenotypes. In particular, the present invention provides methods for the development of plants having agronomically optimized phenotypes by using targeted mutagenesis with few or no off-target effects. Targeted mutagenesis is achieved by the introduction of a base editor complex or of a STEME complex comprising an array of guide RNAs targeting a nucleic acid sequence of interest. The present invention also relates to cellular systems obtained by the methods described herein and to the use of a base editor complex or the STEME complex comprising an array of guide RNAs for generating a cellular system having an agronomically important phenotype and for identification of an agronomically important phenotype.

Abstract: The present invention relates to methods for targeted editing in a plant, a plant cell or material, which is combined with the parallel introduction of a phenotypically selectable trait. Furthermore, methods are provided not comprising a step of introducing a transgenic selection marker sequence.

Abstract: The present invention relates to optimized systems for the genome editing of eukaryotic cells, preferably plant cells. Also provided is a plant delivery system comprising at least one Cpf1 enzyme, or a plant optimized construct encoding the same, to be synergistically combined with a Cpf1 guide RNA system. Further provided is a Cpf1 guide RNA system being flanked by a Hammerhead ribozyme sequence at the 5? end and by a plant-derived Hepatitis Delta Vims (HDV)-like ribozyme sequence at the 3? end, or being embedded within a coding or non-coding region, of a sequence encoding a frame sequence. Novel plant-derived HDV-like ribozyme sequences are also provided. Further provided are methods for improved genome editing, and the use of the various systems provided herein to obtain transformed plants, plant cells, tissues, organs, or a progeny thereof, or a plant material, modified in a targeted way even at difficult to access target sites.

Abstract: The present invention relates to the use of CRISPR/CasX systems in plants for genome engineering, and compositions used in such methods.

Abstract: The present invention relates to improved methods for precision genome editing (GE), preferably in eukaryotic cells, and particularly to methods for GE in cells with specifically altered expression of Polymerase theta and altered characteristics of at least one further enzyme involved in a non-homologous end-joining (NHEJ) DNA repair pathway. Further provided are cellular systems and tools related to the methods provided. Specifically, methods are provided, wherein Polymerase theta and NHEJ blockage and/or GE are performed in a transient way so that the endogenous Polymerase theta and cellular NHEJ machinery is easily reactivated after a targeted edit, and/or without permanent integration of certain editing tools.

Abstract: The subject invention relates to nematode responsive domains obtained from soybean promoters for phosphoribosylformylglycinamidine ribonucleotide (FGAM) synthase paralogs. The nematode responsive domains can be used in promoters that are linked to heterologous DNA. Such constructs can be expressed in transfected or transformed soybean and used in the control of nematode infection of soybean.

Abstract: The subject invention relates to nematode responsive domains obtained from soybean promoters for phosphoribosylformylglycinamidine ribonucleotide (FGAM) synthase paralogs. The nematode responsive domains can be used in promoters that are linked to heterologous DNA. Such constructs can be expressed in transfected or transformed soybean and used in the control of nematode infection of soybean.