Patents by Inventor Zbigniev Skrzypczynski

Zbigniev Skrzypczynski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Oligonucleotide synthesis using periodate salts
    Publication number: 20070049745
    Abstract: The present invention relates generally to nucleic acid chemistry and to the chemical synthesis of oligonucleotides. More particularly, the invention relates to improved methods for synthesizing oligonucleotides wherein periodate salts are used (e.g., in organic solvents) as an oxidation reagent in oligonucleotide synthesis (e.g., for automated phosphoramidite synthesis of oligonucleotides). The invention finds utility in the fields of biochemistry, molecular biology, and pharmacology, and in medical diagnostic and screening technologies.
    Type: Application
    Filed: July 11, 2006
    Publication date: March 1, 2007
    Applicant: Third Wave Technologies, Inc.
    Inventor: Zbigniev Skrzypczynski
  • RNA detection assays
    Patent number: 7150982
    Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5? nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: February 26, 2002
    Date of Patent: December 19, 2006
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim T. Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael W. Kaiser, Robert W. Kwiatkowski, Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lyamicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
  • Flourecent quinacridone derivatives
    Publication number: 20060240452
    Abstract: The present invention relates to methods and compositions utilizing fluorescent quinacridone derivatives. In particular, the present invention relates to the use of fluorescent quinacridone derivatives for the labeling and detection of riucleic acids. The present invention thus provides improved compositions and methods for labeling biological molecules useful in the detection of nucleic acids and other biological molecules.
    Type: Application
    Filed: February 13, 2006
    Publication date: October 26, 2006
    Inventors: Zbigniev Skrzypczynski, Robert Roeven
  • Reactive functional groups
    Publication number: 20050277138
    Abstract: The present invention relates to compositions and methods for the preparation of modified nucleic acids. In particular, the present invention provides novel reagents and chemistries for the generation of linkers, modified phosphoramidites, and modified solid supports.
    Type: Application
    Filed: April 26, 2005
    Publication date: December 15, 2005
    Applicant: Third Wave Technologies, Inc.
    Inventors: Zbigniev Skrzypczynski, Sarah Wayland
  • Nucleic acid synthesizers
    Patent number: 6932943
    Abstract: The present invention relates to nucleic acid synthesizers and methods of using and modifying nucleic acid synthesizers. For example, the present invention provides highly efficient, reliable, and safe synthesizers that find use, for example, in high throughput and automated nucleic acid synthesis, as well as methods of modifying pre-existing synthesizers to improve efficiency, reliability, and safety.
    Type: Grant
    Filed: January 26, 2001
    Date of Patent: August 23, 2005
    Assignee: Third Wave Technologies
    Inventors: Raymond F. Cracauer, Zbigniev Skrzypczynski, Ned D. Reimer, Rocky Ganske
  • Charge tags and the separation of nucleic acid molecules
    Publication number: 20050130179
    Abstract: The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.
    Type: Application
    Filed: June 23, 2004
    Publication date: June 16, 2005
    Applicant: Third Wave Technologies, Inc.
    Inventors: Victor Lyamichev, Zbigniev Skrzypczynski, Hatim Allawi, Sarah Wayland, Tsetska Takova, Bruce Neri, James Prudent, Jeff Hall
  • Nucleic acid analysis methods conducted in small reaction volumes
    Publication number: 20050106596
    Abstract: The present invention relates to reactions conducted on or in solid surface materials, including liquid phase reactions within capillary tubes and arrays of capillary tubes or open end reaction microchambers. A wide array of biomolecular and combinatorial synthesis reactions may be used with the present invention. For example, the present invention provides methods for forming and cleaving nucleic acid cleavage structures on or in solid surface materials.
    Type: Application
    Filed: August 27, 2004
    Publication date: May 19, 2005
    Applicant: Third Wave Technologies, Inc.
    Inventors: Zbigniev Skrzypczynski, Joerg Baier, Karl Nichols
  • Reactive functional groups
    Publication number: 20040219576
    Abstract: The present invention relates to compositions and methods for the preparation of modified nucleic acids. In particular, the present invention provides novel reagents and chemistries for the generation of linkers and modified phosphoramidates.
    Type: Application
    Filed: January 6, 2004
    Publication date: November 4, 2004
    Applicant: Third Wave Technologies, Inc.
    Inventors: Zbigniev Skrzypczynski, Sarah R. Wayland
  • 3' end tagged oligonucleotides
    Publication number: 20040161749
    Abstract: The present invention provides compositions comprising oligonucleotides that have 3′ end groups (e.g. lipophilic moieties) that are useful in invasive cleavage reactions such as the INVADER assay. Specifically, the present invention provides compositions containing oligonucleotides with 3′ end groups configured for generating a detectable signal in invasive cleavages assays with a high signal-to-background ratio, as well as methods for generating such compositions.
    Type: Application
    Filed: January 24, 2003
    Publication date: August 19, 2004
    Inventors: Jeff G. Hall, Zbigniev Skrzypczynski, Sarah Wayland, Ned D. Reimer, Luis P. Reynaldo, Joerg Baier, Victor Lyamichev, Bruce P. Neri
  • RNA detection assays
    Publication number: 20030186238
    Abstract: The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Application
    Filed: February 26, 2002
    Publication date: October 2, 2003
    Inventors: Hatim Allawi, Brad T. Argue, Christian Tor Bartholomay, LuAnne Chehak, Michelle L. Curtis, Peggy S. Eis, Jeff G. Hall, Hon S. Ip, Lin Ji, Michael Kaiser, Robert W. Kwiatkowski, Andrew A. Lukowiak, Victor Lyamichev, Natalie E. Lymaicheva, WuPo Ma, Bruce P. Neri, Sarah M. Olson, Marilyn C. Olson-Munoz, James J. Schaefer, Zbigniev Skrzypczynski, Tsetska Y. Takova, Lisa C. Thompson, Kevin L. Vedvik
  • Polymer synthesizer
    Publication number: 20030124526
    Abstract: The present invention relates to polymer synthesizers and methods of using polymer synthesizers. For example, the present invention provides highly efficient, reliable, and safe synthesizers that find use, for example, in high throughput and automated nucleic acid synthesis. The present invention also relates to synthesizer arrays for efficient, safe, and automated processes for the production of large quantities of polymers.
    Type: Application
    Filed: August 15, 2001
    Publication date: July 3, 2003
    Applicant: Third Wave Technologies, Inc.
    Inventors: Raymond F. Cracauer, Zbigniev Skrzypczynski, Ned D. Reimer
  • Polymer synthesizer
    Publication number: 20030113236
    Abstract: The present invention relates to polymer synthesizers and methods of using polymer synthesizers. For example, the present invention provides highly efficient, reliable, and safe synthesizers that find use, for example, in high throughput and automated nucleic acid synthesis. The present invention also relates to synthesizer arrays for efficient, safe, and automated processes for the production of large quantities of polymers.
    Type: Application
    Filed: August 15, 2001
    Publication date: June 19, 2003
    Applicant: Third Wave Technologies, Inc.
    Inventors: Raymond F. Cracauer, Zbigniev Skrzypczynski, Ned D. Reimer
  • Polymer synthesizer
    Publication number: 20030113237
    Abstract: The present invention relates to polymer synthesizers and methods of using polymer synthesizers. For example, the present invention provides highly efficient, reliable, and safe synthesizers that find use, for example, in high throughput and automated nucleic acid synthesis. The present invention also relates to synthesizer arrays for efficient, safe, and automated processes for the production of large quantities of polymers.
    Type: Application
    Filed: August 15, 2001
    Publication date: June 19, 2003
    Applicant: Third Wave Technologies, Inc.
    Inventors: Raymond F. Cracauer, Zbigniev Skrzypczynski, Ned D. Reimer
  • Polymer synthesizer
    Publication number: 20030072689
    Abstract: The present invention relates to polymer synthesizers and methods of using polymer synthesizers. For example, the present invention provides highly efficient, reliable, and safe synthesizers that find use, for example, in high throughput and automated nucleic acid synthesis. The present invention also relates to synthesizer arrays for efficient, safe, and automated processes for the production of large quantities of polymers.
    Type: Application
    Filed: August 15, 2001
    Publication date: April 17, 2003
    Applicant: Third Wave Technologies, Inc.
    Inventors: Raymond F. Cracauer, Zbigniev Skrzypczynski, Ned D. Reimer