Patents by Inventor Zebin LIN

Zebin LIN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • MYOGENIN-EXPRESSING FIBROBLAST-LIKE CELL (MEFLC) LINE AND CONSTRUCTION METHOD AND USE THEREOF
    Publication number: 20240034997
    Abstract: A myogenin-expressing fibroblast-like cell (MEFLC) line and a construction method and a use thereof are provided. The MEFLC line was deposited in the Guangdong Microbial Culture Collection Center (GDMCC) on Apr. 23, 2022 with an accession number of GDMCC NO: 62409. The construction method includes: 1) constructing a pCW-MYOG-T2A-Puro lentivirus; 2) infecting a human induced pluripotent stem cell (iPSC) line with the lentivirus, inducing the expression of an MYOG gene, and screening out a positive monoclonal cell line; 3) subjecting the positive monoclonal cell line to expanded cultivation, during which the expression of the MYOG gene is continuously induced; and 4) changing cultivation conditions, continuously inducing the expression of the MYOG gene, and screening out positive monoclonal cell lines to ensure the purity of MYOG-positive cells until a cell morphology changes significantly into fibroblastoid cells to obtain the MEFLC line.
    Type: Application
    Filed: March 31, 2023
    Publication date: February 1, 2024
    Applicant: Foshan Zhongke Rhythm Biotech Co.,Ltd.
    Inventors: Bin LIN, Weiwei KONG, Lishi ZHOU, Ping WANG, Zebin LIN, Qiang GAO, Jianzheng CEN, Jian ZHUANG