Abstract: Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system.

Abstract: Disclosed is a sensor device with pixels that in addition to sensing an image, performs captured image or scene analysis. The device saves further image processing downstream, is characterized by minimal charge movements and reduces a large fraction (about 90%) of the consumed electric energy as compared to the existing devices.

Abstract: A universal counting and measurement system and method. The system and method are not restricted to a specific application, aimed at counting a specific type of object, but are capable of counting objects of a large variety of sizes and shapes. According to certain embodiments, the system and method are also capable of measuring volumes of materials held within a container or piled on a surface. The shape of the containers or the shape of the pile of material are not limited to a certain prescribed architecture. According to certain embodiments, the system is easily adapted to the different applications and can be made ready to work in a short time. The system is also capable of counting accumulating objects or measuring accumulating volumes within one or more containers or piles.

Abstract: There are provided methods for detection and measurement of stress in a cell, the method including introducing a labeled tRNA into the cell and detecting a change in subcellular localization of the labeled tRNA in the cell, based on the signal emitted from the labeled tRNA. There are further provided methods and systems for the generation of a stress index of a living cell. There are further provided methods and systems for detection of stress in a living cell, comprising detection of changes in subcellular localization of labeled tRNA in a cell, wherein the detection is performed in real time.

Abstract: A method and a device are disclosed for monitoring the synthesis of proteins by the ribosome, wherein the ribosome is bound to a first label, for example a donor fluorophore, and a tRNA and/or amino acid are is bound to a second label, for example an acceptor fluorophore, wherein the first and second labels together from a FRET pair. As the ribosome mechanism processes the mRNA and tRNA molecules and synthesizes a polypeptide chain, a light source illuminates the ribosome, exciting the donor fluorophores and thereby the acceptor fluorophores whenever these are in sufficient proximity to a donor. The resulting signals are detected and used as a key for database searching and identification of the protein being synthesized.

Abstract: The present invention relates to systems and methods for measuring the rate of translation of a target protein in cells, which are based on the detection of translation of one or more predetermined codon pairs during synthesis of the target protein. The detection is provided by a FRET signal emitted from labeled tRNA molecules which are juxtaposed during synthesis of the protein.

Abstract: Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system.

Abstract: A system and method of inspecting electrical circuits with multiple optical inputs, including: obtaining first and second image data that are generally spatially coincidental but which each include some image data that is different, modifying one of the images by employing the other image so as to produce an enhanced representation of the electrical circuit, and inspecting the enhanced representation for defects.

Abstract: A miniature autonomous apparatus for performing scene interpretation, comprising: image acquisition means, image processing means, memory means and communication means, the processing means comprising means for determining an initial parametric representation of the scene; means for updating the parametric representation according to predefined criteria; means for analyzing the image, comprising means for determining, for each pixel of the image, whether it is a hot pixel, according to predefined criteria; means for defining at least one target from the hot pixels; means for measuring predefined parameters for at least one target; and means for determining, for at least one target whether said target is of interest, according to application-specific criteria, and wherein said communication means are adapted to output the results of said analysis.

Abstract: A method and a device are disclosed for monitoring the synthesis of proteins by the ribosome, wherein the ribosome is bound to a first label, for example a donor fluorophore, and a tRNA and/or amino acid are is bound to a second label, for example an acceptor fluorophore, wherein the first and second labels together from a FRET pair. As the ribosome mechanism processes the mRNA and tRNA molecules and synthesizes a polypeptide chain, a light source illuminates the ribosome, exciting the donor fluorophores and thereby the acceptor fluorophores whenever these are in sufficient proximity to a donor. The resulting signals are detected and used as a key for database searching and identification of the protein being synthesized.

Abstract: A method and a device are disclosed for monitoring the synthesis of proteins by the ribosome in real time, in vivo as well as in in-vitro. The ribosome is engineered to carry a donor fluorophore, and tRNA and/or amino acids and/or some other part of the ribosome are either engineered to carry acceptor fluorophores or else their natural fluorescent properties are utilized as acceptors. As the ribosomes mechanism processed the mRNA and tRNA molecules and synthesizes a polypeptide chain, a light source illuminates the ribosome, exciting the donor fluorophores and thereby the acceptor fluorophores whenever these are in sufficient proximity to a donor. The resulting signals are detected and used as a key for real-time database searching and identification of the protein being synthesized. The resulting data can be tabulated and interpreted in different ways. FIG. (1) describes the properties of a FRET pair and the dependence of FRET on pair distance.

Abstract: A universal counting and measurement system and method. The system and method are not restricted to a specific application, aimed at counting a specific type of object, but are capable of counting objects of a large variety of sizes and shapes. According to certain embodiments, the system and method are also capable of measuring volumes of materials held within a container or piled on a surface. The shape of the containers or the shape of the pile of material are not limited to a certain prescribed architecture. According to certain embodiments, the system is easily adapted to the different applications and can be made ready to work in a short time. The system is also capable of counting accumulating objects or measuring accumulating volumes within one or more containers or piles.

Abstract: A miniature autonomous apparatus for performing scene interpretation, comprising: image acquisition means, image processing means, memory means and communication means, the processing means comprising means for determining an initial parametric representation of the scene; means for updating the parametric representation according to predefined criteria; means for analyzing the image, comprising means for determining, for each pixel of the image, whether it is a hot pixel, according to predefined criteria; means for defining at least one target from the hot pixels; means for measuring predefined parameters for at least one target; and means for determining, for at least one target whether said target is of interest, according to application-specific criteria, and wherein said communication means are adapted to output the results of said analysis.

Abstract: A miniature autonomous apparatus for performing scene interpretation, comprising: image acquisition means, image processing means, memory means and communication means, the processing means comprising means for determining an initial parametric representation of the scene; means for updating the parametric representation according to predefined criteria; means for analyzing the image, comprising means for determining, for each pixel of the image, whether it is a hot pixel, according to predefined criteria; means for defining at least one target from the hot pixels; means for measuring predefined parameters for at least one target; and means for determining, for at least one target whether said target is of interest, according to application-specific criteria, and wherein said communication means are adapted to output the results of said analysis.

Abstract: A method is provided for the detection of defects on a semiconductor wafer by checking individual pixels on the wafer, collecting the signature of each pixel, defined by the way in which it responds to the light of a scanning beam, and determining whether the signature is that of a faultless pixel or of a pixel that is defective or suspect to be defective. An apparatus is also provided for the determination of such defects, which comprises a stage for supporting a wafer, a laser source generating a beam that is directed onto the wafer, collecting optics and photoelectric sensors for collecting the laser light scattered by the wafer in a number of directions and generating corresponding analog signals, an A/D converter deriving from the signals digital components defining pixel signatures, and selection systems for identifying the signatures of suspect pixels and verifying whether the suspect pixels are indeed defective.

Abstract: An electrical circuit inspection method including, for each of a plurality of types of local characteristics, each type occurring at least once within electrical circuitry to be inspected, identifying at least one portion of interest within the electrical circuitry whereat the local characteristic is expected to occur, and inspecting an image of each portion of interest, using an inspection task selected in response to the type of local characteristic expected to occur in the portion of interest.

Abstract: A system and method of inspecting electrical circuits with multiple optical inputs, including: obtaining first and second image data that are generally spatially coincidental but which each include some image data that is different, modifying one of the images by employing the other image so as to produce an enhanced representation of the electrical circuit, and inspecting the enhanced representation for defects.

Abstract: A system and method of inspecting electrical circuits with multiple optical inputs, including: obtaining first and second image data that are generally spatially coincidental but which each include some image data that is different, modifying one of the images by employing the other image so as to produce an enhanced representation of the electrical circuit, and inspecting the enhanced representation for defects.

Abstract: A method for inspecting objects comprising: creating a reference image for a representative object, the reference image comprising an at least partially vectorized first representation of boundaries representing the representative object; acquiring an image of an object under inspection comprising a second representation of boundaries representing the object under inspection; and comparing a location of at least some boundaries in the second representation of boundaries to a location of corresponding boundaries in the at least partially vectorized first representation of boundaries, thereby to identify defects.

Abstract: A method for inspecting electrical circuits, and a system for carrying out the method, generating a representation of boundaries of elements in an image of an electrical circuit which is under inspection, and analyzing at least some locations of at least some boundaries in the representation of boundaries of elements to identify defects in the electrical circuit.