Patents by Inventor Zefei Jiang
Zefei Jiang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11931742Abstract: A nucleic acid loading device, including a housing, a carrying module, a motion module, a liquid circuit module and a control module. The motion module includes a support platform, first and second driving mechanisms, and a carrying member. The liquid circuit module is configured to control a reagent to flow into and out of a reactor. The control module is connected with the motion module and the liquid circuit module, and the control module is configured to control the first and second driving mechanisms, the liquid circuit module and the temperature control unit. A first face of a semiconductor cooler heats or refrigerates during operation, providing heat for an accommodating seat through a heat conducting body such that the reactor can be at different ambient temperatures. The cooperation between the motion and liquid circuit modules may achieve automation and industrialization of loading a sample into the reactor.Type: GrantFiled: December 12, 2018Date of Patent: March 19, 2024Assignee: GENEMIND BIOSCIENCES COMPANY LIMITEDInventors: Songzhen Zhang, Zefei Jiang, Guangming Wang, Ping Wu, Zhiliang Zhou, Qin Yan
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Publication number: 20240017262Abstract: A liquid path system (12), comprising a flow path selection valve (10), a pump assembly (14), and a fluid network (60). The fluid network (60) comprises a reservoir (64), a first flow channel, a second flow channel, and a reaction device (62). The first flow channel and the second flow channel are each independently in flow communication with the reservoir (64) and the reaction device (62). The pump assembly (14) is in communication with the flow path selection valve (10). The pump assembly (14) is in communication with the fluid network (60). The flow path selection valve (10) is configured to rotate between a first valve position and a second valve position.Type: ApplicationFiled: September 24, 2021Publication date: January 18, 2024Inventors: Xuefeng WAN, Ping WU, Zefei JIANG
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Patent number: 11874453Abstract: An optical system including a first light source, a first lens, and a light splitting module, wherein the light splitting module has a first splitter, a second lens, a first camera and a second camera, the first lens is configured for receiving a first light beam from the first light source and collimating the first light beam onto a sample, and for receiving and collimating a light beam from the sample, the second lens is configured for focusing the collimated light beam from the first lens to the first camera and the second camera, the first splitter is configured for splitting the focused light beam from the second lens into a second light beam and a third light beam, the first camera is configured for receiving the second light beam, and the second camera is configured for receiving the third light beam.Type: GrantFiled: September 29, 2019Date of Patent: January 16, 2024Assignee: GENEMIND BIOSCIENCES COMPANY LIMITEDInventors: Jifang Qi, Zhiliang Zhou, Zefei Jiang, Qin Yan
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Patent number: 11847766Abstract: A method and device for detecting bright spots on an image. The image is acquired from a field of view where a base extension reaction occurs, multiple nucleic acid molecules with optically detectable labels exist in the field of view where the base extension reaction occurs, and at least some of the nucleic acid molecules appear as bright spots on the image. The method includes preprocessing the image to obtain a preprocessed image; determining a critical value to simplify the preprocessed image to obtain a simplified image, determining a first bright spot detection threshold on the basis of the preprocessed image, and identifying candidate bright spots on the image on the basis of the preprocessed image and the simplified image, including determining a pixel matrix that satisfies at least two of conditions as a candidate bright spot.Type: GrantFiled: August 22, 2018Date of Patent: December 19, 2023Assignee: GENEMIND BIOSCIENCES COMPANY LIMITEDInventors: Linsen Li, Weibin Xu, Huan Jin, Zefei Jiang, Zhiliang Zhou, Qin Yan
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Patent number: 11575823Abstract: The present disclosure discloses a method and a system for imaging. The method for imaging objects using the system for imaging. The system for imaging comprises a lens. The objects comprise a first object, a second object and a third object located at different positions on a first preset track. The method for imaging comprises: allowing the lens and the first preset track to move relatively in a first predetermined relationship to acquire a clear image of the third object using the system for imaging without focusing, the first predetermined relationship is determined by a focal plane position of the first object and a focal plane position of the second object. The aforementioned method for imaging is high in imaging efficiency and is capable of fast focusing according to the first predetermined relationship even if focus tracking fails so that the blurring of a photographed image due to defocusing is avoided.Type: GrantFiled: May 17, 2022Date of Patent: February 7, 2023Assignee: GeneMind Biosciences Company LimitedInventors: Linsen Li, Ruitao Sun, Jiahong Xu, Zhiliang Zhou, Zefei Jiang, Qin Yan
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Publication number: 20230027811Abstract: A method for identifying a base in nucleic acid, a computer-readable storage medium, a computer program product, and a system. The method for identifying a base in nucleic acid comprises: mapping a coordinate of each bright spot in a bright spot set corresponding to a template onto an image to be inspected, and determining the position of a corresponding coordinate on said image (S11); determining the intensity of a signal at the position of the corresponding coordinate on said image, the intensity being a corrected intensity (S21); and comparing the intensity of the signal at the position of the corresponding coordinate on said image with the size of a first preset value, and determining a base type corresponding to the position on the basis of the comparison result, so as to achieve base calling (S31). The method may quickly and accurately identify a base, and achieve the determination of an order of nucleotides/bases of at least part of a sequence of a template.Type: ApplicationFiled: September 10, 2020Publication date: January 26, 2023Inventors: Linsen LI, Huan JIN, Zefei JIANG, Lei SUN
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Publication number: 20220373780Abstract: A vibration damping structure (60), a detection system and a sequencing system. The vibration damping structure (60) is used in the detection system. The vibration damping structure (60) comprises a main body (62) and a support body (64), the main body (62) is connected to the detection system by means of the support body (64), the main body (62) comprises an imaging module (10), an upper layer structure (66), a lower layer structure (68) and an intermediate structure (70), the imaging module (10) is mounted on the upper layer structure (66), the lower layer structure (68) bears the upper layer structure (66) by means of the intermediate structure (70), and the natural frequency of the main body (62) is greater than or equal to ?{square root over (2)} times the internal excitation frequency.Type: ApplicationFiled: July 14, 2020Publication date: November 24, 2022Inventors: Jiao ZHENG, Zefei JIANG, Zhiliang ZHOU, Guangming WANG, Songzhen ZHANG
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Patent number: 11482304Abstract: The disclosure discloses an alignment method, device, and system.Type: GrantFiled: August 2, 2017Date of Patent: October 25, 2022Assignee: GENEMIND BIOSCIENCES COMPANY LIMITEDInventors: Weibin Xu, Huan Jin, Qin Yan, Zefei Jiang, Zhiliang Zhou
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Publication number: 20220298567Abstract: The present disclosure discloses a method for controlling a base sequence determination, a base sequence determination system, and a control device. The base sequence determination system includes a fluid device and an optical device, a reaction device includes a first component and a second component, and a repeated executable unit included in the base sequence determination is defined as: a second biochemical reaction—a first biochemical reaction—photographing. The method includes, after initiation steps are completed, using the fluid device to perform the second biochemical reaction and the first biochemical reaction of the sample on the first component, while using the optical device to photograph the sample on the second component. The initial steps include: a. using the fluid device to perform the first biochemical reaction of the sample on the first component, b. using the optical device to photograph the sample on the first component after the first biochemical reaction, and c.Type: ApplicationFiled: June 10, 2022Publication date: September 22, 2022Inventors: Jianfeng Xu, Ruitao Sun, Zefei Jiang, Zhiliang Zhou, Qin Yan
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Publication number: 20220279130Abstract: The present disclosure discloses a method and a system for imaging. The method for imaging objects using the system for imaging. The system for imaging comprises a lens. The objects comprise a first object, a second object and a third object located at different positions on a first preset track. The method for imaging comprises: allowing the lens and the first preset track to move relatively in a first predetermined relationship to acquire a clear image of the third object using the system for imaging without focusing, the first predetermined relationship is determined by a focal plane position of the first object and a focal plane position of the second object. The aforementioned method for imaging is high in imaging efficiency and is capable of fast focusing according to the first predetermined relationship even if focus tracking fails so that the blurring of a photographed image due to defocusing is avoided.Type: ApplicationFiled: May 17, 2022Publication date: September 1, 2022Inventors: Linsen LI, Ruitao SUN, Jiahong XU, Zhiliang ZHOU, Zefei JIANG, Qin YAN
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Patent number: 11421272Abstract: Disclosed are a positioning method, a positioning apparatus and a sequencing system (300). The sequencing system (300) comprises an imaging assembly (110) and a mobile platform (102), wherein the imaging assembly (110) comprises an optical axis (OP), the mobile platform (102) bears a reaction apparatus (200), the stroke of the mobile platform (102) in a first direction is controlled by means of a limiting switch, and the stroke comprises a first limit.Type: GrantFiled: September 29, 2019Date of Patent: August 23, 2022Assignee: GeneMind Biosciences Company LimitedInventors: Ruitao Sun, Songzhen Zhang, Zefei Jiang, Zhiliang Zhou, Qin Yan
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Patent number: 11384390Abstract: The present disclosure relates to base sequence determination. A base sequence determination system includes a fluid device and an optical device, a reaction device includes a first component and a second component, and a repeated executable unit is defined as: a second biochemical reaction—a first biochemical reaction—photographing. A method includes, after initiation steps, using the fluid device to perform the second biochemical reaction and the first biochemical reaction of the sample on the first component, while using the optical device to photograph the sample on the second component. The initial steps include: using the fluid device to perform the first biochemical reaction of the sample on the first component, using the optical device to photograph the sample on the first component after the first biochemical reaction, and using the fluid device to perform the first biochemical reaction of the sample on the second component.Type: GrantFiled: December 27, 2017Date of Patent: July 12, 2022Assignee: GeneMind Biosciences Company LimitedInventors: Jianfeng Xu, Ruitao Sun, Zefei Jiang, Zhiliang Zhou, Qin Yan
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Patent number: 11368614Abstract: The present disclosure discloses a method and a system for imaging. The method for imaging objects using the system for imaging. The system for imaging comprises a lens. The objects comprise a first object, a second object and a third object located at different positions on a first preset track. The method for imaging comprises: allowing the lens and the first preset track to move relatively in a first predetermined relationship to acquire a clear image of the third object using the system for imaging without focusing, the first predetermined relationship is determined by a focal plane position of the first object and a focal plane position of the second object. The aforementioned method for imaging is high in imaging efficiency and is capable of fast focusing according to the first predetermined relationship even if focus tracking fails so that the blurring of a photographed image due to defocusing is avoided.Type: GrantFiled: July 23, 2019Date of Patent: June 21, 2022Assignee: GeneMind Biosciences Company LimitedInventors: Linsen Li, Ruitao Sun, Jiahong Xu, Zhiliang Zhou, Zefei Jiang, Qin Yan
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Publication number: 20220090190Abstract: Disclosed are a positioning method, a positioning apparatus and a sequencing system (300). The sequencing system (300) comprises an imaging assembly (110) and a mobile platform (102), wherein the imaging assembly (110) comprises an optical axis (OP), the mobile platform (102) bears a reaction apparatus (200), the stroke of the mobile platform (102) in a first direction is controlled by means of a limiting switch, and the stroke comprises a first limit.Type: ApplicationFiled: September 29, 2019Publication date: March 24, 2022Inventors: Ruitao SUN, Songzhen ZHANG, Zefei JIANG, Zhiliang ZHOU, Qin YAN
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Publication number: 20210373314Abstract: An optical system including a first light source, a first lens, and a light splitting module, wherein the light splitting module has a first splitter, a second lens, a first camera and a second camera, the first lens is configured for receiving a first light beam from the first light source and collimating the first light beam onto a sample, and for receiving and collimating a light beam from the sample, the second lens is configured for focusing the collimated light beam from the first lens to the first camera and the second camera, the first splitter is configured for splitting the focused light beam from the second lens into a second light beam and a third light beam, the first camera is configured for receiving the second light beam, and the second camera is configured for receiving the third light beam.Type: ApplicationFiled: September 29, 2019Publication date: December 2, 2021Inventors: Jifang QI, Zhiliang ZHOU, Zefei JIANG, Qin YAN
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Patent number: 11170506Abstract: A method for constructing a sequencing template based on an image, a device, and a system. The image includes first, second, third and fourth images of one same field of view corresponding to base extensions of A, T/U, G, and C respectively; the first, second, third and fourth images respectively include images M1 and M2, images N1 and N2, images P1 and P2, and images Q1 and Q2; the method includes combining any two of the images M1, M2, N1, N2, P1, P2, Q1, and Q2to perform bright spot matching, and enabling such images to participate in the combination for at least one time to obtain a plurality of combined images including first coincident bright spots, and merging the first coincident bright spots on the plurality of combined images to obtain a bright spot set corresponding to the sequencing template.Type: GrantFiled: August 22, 2018Date of Patent: November 9, 2021Assignee: GENEMIND BIOSCIENCES COMPANY LIMITEDInventors: Linsen Li, Weibin Xu, Huan Jin, Zefei Jiang, Zhiliang Zhou, Qin Yan
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Publication number: 20210266469Abstract: The present disclosure discloses a method and a system for imaging. The method for imaging objects using the system for imaging. The system for imaging comprises a lens. The objects comprise a first object, a second object and a third object located at different positions on a first preset track. The method for imaging comprises: allowing the lens and the first preset track to move relatively in a first predetermined relationship to acquire a clear image of the third object using the system for imaging without focusing, the first predetermined relationship is determined by a focal plane position of the first object and a focal plane position of the second object. The aforementioned method for imaging is high in imaging efficiency and is capable of fast focusing according to the first predetermined relationship even if focus tracking fails so that the blurring of a photographed image due to defocusing is avoided.Type: ApplicationFiled: July 23, 2019Publication date: August 26, 2021Inventors: Linsen LI, Ruitao SUN, Jiahong XU, Zhiliang ZHOU, Zefei JIANG, Qin YAN
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Publication number: 20210217186Abstract: A method and device for image registration, and a computer program product. The method includes performing, based on a reference image, a first registration of an image to be registered, including determining a first offset between predetermined areas on the image and the reference image, and moving all bright spots on the image based on the first offset; performing, based on the reference image, a second registration with respect to the first registered image, including merging the first registered image and the reference image, calculating the offset of all overlapped bright spots in the predetermined area on the merged image to determine a second offset, two or more bright spots distanced by less than a predetermined number of pixels being one overlapped bright spot, and moving, on the basis of the second offset, all bright spots on the first registered image, thus implementing the registration of the image.Type: ApplicationFiled: August 22, 2018Publication date: July 15, 2021Inventors: Linsen LI, Weibin XU, Huan JIN, Zefei JIANG, Zhiliang ZHOU, Qin YAN
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Publication number: 20210217177Abstract: A method and device for detecting bright spots on an image. The image is acquired from a field of view where a base extension reaction occurs, multiple nucleic acid molecules with optically detectable labels exist in the field of view where the base extension reaction occurs, and at least some of the nucleic acid molecules appear as bright spots on the image. The method includes preprocessing the image to obtain a preprocessed image, determining a critical value to simplify the preprocessed image to obtain a simplified image, determining a first bright spot detection threshold on the basis of the preprocessed image, and identifying candidate bright spots on the image on the basis of the preprocessed image and the simplified image, including determining a pixel matrix that satisfies at least two of conditions as a candidate bright spot.Type: ApplicationFiled: August 22, 2018Publication date: July 15, 2021Inventors: Linsen LI, Weibin XU, Huan JIN, Zefei JIANG, Zhiliang ZHOU, Qin YAN
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Publication number: 20210217171Abstract: A method for constructing a sequencing template based on an image, a device, and a system. The image includes first, second, third and fourth images of one same field of view corresponding to base extensions of A, T/U, G, and C respectively; the first, second, third and fourth images respectively include images M1 and M2, images N1 and N2, images P1 and P2, and images Q1 and Q2; the method includes combining any two of the images M1, M2, N1, N2, P1, P2, Q1, and Q2 to perform bright spot matching, and enabling such images to participate in the combination for at least one time to obtain a plurality of combined images including first coincident bright spots, and merging the first coincident bright spots on the plurality of combined images to obtain a bright spot set corresponding to the sequencing template.Type: ApplicationFiled: August 22, 2018Publication date: July 15, 2021Inventors: Linsen LI, Weibin XU, Huan JIN, Zefei JIANG, Zhiliang ZHOU, Qin YAN