Abstract: Disclosed is a simple and cost-effective technique for generation of high-throughput aerosols of uniform-diameter submillimeter-size core-shell particles. An aerosol may be created by using, e.g., a first tube filled with liquid and having a small hole through a sidewall, then passing a fluid through the liquid via a second tube passing partially though the first tube at a location above the small hole, forming coaxial flow through the small hole. The diameter of generated core-shell particles scales with the inner and outer diameter of the gas tube nozzle, enabling control on the size of the produced particles. Further disclosed is a simple, scalable and cost-effective technique that enables microencapsulation of various materials. Including highly viscous materials, into sub-10 ?m particles. A specially designed atomizing tube interacts with bubbles formed in a liquid comprising a plurality of immiscible liquid layers to generate aerosols of droplets which have layered core-shell structure.

Abstract: Described herein is a method for encapsulating single cells using alternating current electrospray technology in tip streaming mode. The encapsulation efficiency is over 80% and natural (alginate, collagen) and synthetic (NorHA) hydrogels and various cell types can be used. The encapsulated cells can be implanted and are protected from the host's immune response. In addition, the coating allows better tissue growth in laboratory cell cultures with a conformal mechanical support that allows molecular and nutrient transport.

Abstract: Systems and methods for fibrous material manufacturing are provided. The methods include dispensing a first stream of a solution (that includes a crosslinkable material) from first nozzle(s) into a bath containing a liquid (that includes a first material). The first nozzle(s) are submerged in the liquid. The methods include dispensing a second stream from second nozzle(s) also submerged in the liquid. The second stream(s) are configured to elongate and thin the first stream(s). The second stream contain a liquid. The liquid includes a second material, which may be the same, or may be different, form the first material. The methods include forming a fibrous material by crosslinking the crosslinkable material in the first stream (e.g., using a light source to cross-link a photo-crosslinkable material in the stream).

Abstract: Described herein is a method for encapsulating single cells using alternating current electrospray technology in tip streaming mode. The encapsulation efficiency is over 80% and natural (alginate, collagen) and synthetic (NorHA) hydrogels and various cell types can be used. The encapsulated cells can be implanted and are protected from the host's immune response. In addition, the coating allows better tissue growth in laboratory cell cultures with a conformal mechanical support that allows molecular and nutrient transport.

Abstract: The invention provides an alternating current electrospray technology that can generate micron sized droplets in oil at very high throughput for emulsion or digital PCR (Polymerase Chain Reaction). This technology outperforms the throughput of the current gold standard in droplet generation using flow-focusing technology by at least a factor of 100. The design is simple and can generate a billion to a trillion monodispersed droplets in about one hour. This is much faster than flow-focusing which is limited to a few million droplets per hour. The droplet size and generation rate can also be easily adjusted by changing the voltage of the AC electric field. The range of produced droplet sizes is about 1-100 microns, wherein the droplets are monodispersed in size.

Abstract: The invention provides an alternating current electrospray technology that can generate micron sized droplets in oil at very high throughput for emulsion or digital PCR (Polymerase Chain Reaction). This technology outperforms the throughput of the current gold standard in droplet generation using flow-focusing technology by at least a factor of 100. The design is simple and can generate a billion to a trillion monodispersed droplets in about one hour. This is much faster than flow-focusing which is limited to a few million droplets per hour. The droplet size and generation rate can also be easily adjusted by changing the voltage of the AC electric field. The range of produced droplet sizes is about 1-100 microns, wherein the droplets are monodispersed in size.