Abstract: The present invention relates to the fields of genetic engineering and fermentation engineering, and provides a Candida utilis double gene co-expression strain for hydrolyzing protein components in kitchen waste, in which the Candida utilis double gene co-expression strain is constructed by integrating carboxypeptidases and endoprotease genes through a Candida utilis expression vector onto a Candida utilis genome. The present invention further provides a Candida utilis double gene co-expression strain capable of degrading kitchen waste, specifically degrading the protein components in the kitchen waste, and decomposing and transforming the proteins into small peptides and amino acids.

Abstract: Disclosed is a genetically recombinant Saccharomyces cerevisiae useful for degrading and utilizing kitchen wastes. Genes encoding ?-amylase (AMY), glucoamylase (GA) and acid protease (AP) were introduced into the genetically recombinant Saccharomyces cerevisiae using a Saccharomyces cerevisiae multi-gene co-expression vector and successfully expressed and secreted. The Saccharomyces cerevisiae so obtained are capable of secreting amylases and protease to degrade the starch and proteins in kitchen wastes to produce carbon and nitrogen sources such as glucose, polypeptides and amino acids, allowing fermentation into ethanol.

Abstract: Provided is a genetically engineered Candida utilis capable of degrading and utilizing kitchen waste. The genetically engineered Candida utilis is obtained by using a Candida utilis multigene co-expression vector to integrate alpha-amylase, glucoamylase and acid protease genes into the Candida utilis genome and to correctly express such three enzymes.

Abstract: Provided is a genetically engineered Candida utilis capable of degrading and utilizing kitchen waste. The genetically engineered Candida utilis is obtained by using a Candida utilis multigene co-expression vector to integrate alpha-amylase, glucoamylase and acid protease genes into the Candida utilis genome and to correctly express such three enzymes.

Abstract: Disclosed is a genetically recombinant Saccharomyces cerevisiae useful for degrading and utilizing kitchen wastes. Genes encoding ?-amylase(AMY), glucoamylase (GA) and acid protease (AP) were introduced into the genetically recombinant Saccharomyces cerevisiae using a saccharomyces cerevisiae multi-gene co-expression vector and successfully expressed and secreted. The Saccharomyces cerevisiae so obtained are capable of secreting amylases and protease to degrade the starch and proteins in kitchen wastes to produce carbon and nitrogen sources such as glucose, polypeptides and amino acids, allowing fermentation into ethanol.