Patents by Inventor Zengpeng HAN

Zengpeng HAN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method, system and recombinant bacmid for preparation of recombinant adeno-associated virus
    Patent number: 11708585
    Abstract: A method of preparing a recombinant adeno-associated virus (rAAV) including: (1) preparing a shuttle plasmid and a corresponding recombinant bacmid including a baculovirus genome, where the shuttle plasmid includes at least an rAAV gene of interest flanked by inverted terminal repeats (ITR-GOI) integrated with a heterologous functional gene fragment, and the recombinant bacmid includes an expression cassette of functional protein components necessary for assembly of the rAAV; (2) integrating the rAAV ITR-GOI and the expression cassette of functional protein components by using the shuttle plasmid and the recombinant bacmid, to yield a recombinant bacmid including a recombinant baculovirus genome; and (3) transfecting, with the recombinant bacmid, a host cell line.
    Type: Grant
    Filed: June 28, 2019
    Date of Patent: July 25, 2023
    Assignee: WUHAN INSTITUTE OF PHYSICS AND MATHEMATICS, CHINESE ACADEMY OF SCIENCES
    Inventors: Yang Wu, Fuqiang Xu, Ting Mei, Liangyu Jiang, Zengpeng Han
  • METHOD, SYSTEM AND RECOMBINANT BACMID FOR PREPARATION OF RECOMBINANT ADENO-ASSOCIATED VIRUS
    Publication number: 20200208175
    Abstract: A method of preparing a recombinant adeno-associated virus (rAAV) including: (1) preparing a shuttle plasmid and a corresponding recombinant bacmid including a baculovirus genome, where the shuttle plasmid includes at least an rAAV gene of interest flanked by inverted terminal repeats (ITR-GOI) integrated with a heterologous functional gene fragment, and the recombinant bacmid includes an expression cassette of functional protein components necessary for assembly of the rAAV; (2) integrating the rAAV ITR-GOI and the expression cassette of functional protein components by using the shuttle plasmid and the recombinant bacmid, to yield a recombinant bacmid including a recombinant baculovirus genome; and (3) transfecting, with the recombinant bacmid, a host cell line.
    Type: Application
    Filed: June 28, 2019
    Publication date: July 2, 2020
    Inventors: Yang WU, Fuqiang XU, Ting MEI, Liangyu JIANG, Zengpeng HAN