Abstract: The present disclosure provides a single nucleotide polymorphism (SNP) molecular marker combination for genetic relationship identification of a Huaxi cattle, use, and an identification method, and belongs to the technical field of genotyping. The present disclosure provides a SNP molecular marker combination for genetic relationship identification of a Huaxi cattle, where the genetic relationship identification of the Huaxi cattle is completed with at least 70 of 1,000 sites. In the present disclosure, the 1,000 sites are the SNP molecular markers distributed on 29 autosomes, and two adjacent SNP molecular markers on a same autosome have an average distance of 2 Mb. A minimum allele frequency (MAF), an expected heterozygosity (HExp), and a polymorphism information content (PIC) of the SNP molecular marker combination have average values of 0.4846, 0.4983, and 0.3741, respectively; and when a maternal genotype is unknown, there is a cumulative probability of exclusion (CPE) of 0.9999999999999.

Abstract: The present disclosure provides a genomic selection (GS) breeding chip of Huaxi cattle and use thereof, and relates to the technical field of molecular breeding. The present disclosure provides a molecular marker combination for whole-genome genotyping of Huaxi cattle, where a genotyping object involves 112,177 single nucleotide polymorphisms (SNPs) and 3 Indels, including seven marker panels in total. In the present disclosure, a genome-wide breeding chip constructed with the molecular marker combination has functional relevance, pertinence and effectiveness, innovation, comprehensiveness, practicality, and cost-effectiveness. This breeding chip also shows uniform distribution of chromosomes in the whole-genome, high coverage, and moderate throughput of markers. This breeding chip has desirable compatibility with existing commercial chips and high cost performance.