Abstract: A comprehensive reconstruction method for long-series sediment data in data-lacking areas includes steps of: collecting hydrological and sediment data of a target river section; calculating sediment data in data-rich years with a flow-sediment content annual relationship curve method; calculating sediment data in only water quality and sediment test years with a correlation method between water quality and sediment data and hydrological station sediment data; calculating sediment data in data-lacking years with an adjacent station same year flow-sediment content relationship curve method; and calculating sediment data in remaining years with a multi-year average flow-sediment content relationship curve method.

Abstract: The invention relates to a set of oligonucleotide primers and their use in methods for amplifying and detecting oligonucleotides, detecting pathogens, or diagnosing infections such as SARS-CoV-2 and Covid-19. The primer set includes a switch oligonucleotide that is adapted to anneal to a forward or reverse primer of the set at temperatures that are below the temperature range for amplification of the DNA. The switch oligonucleotide prevents amplification from the complementary primer when the complementary primer is bound to the switch oligonucleotide. The invention also provides a method of reducing false positives in the detection of a target DNA or RNA sequence using loop-mediated isothermal amplification (LAMP) or reverse transcription loop-mediated isothermal amplification (RT-LAMP), by using such a switch oligonucleotide.

Abstract: A macrocarrier for the propagation of biological cells is described. The macrocarrier comprises substrate particles that are coated with a thermoresponsive polymer, which is capable of providing the macrocarrier with a cell-receiving surface and responding to a change in temperature to release cells from the macrocarrier. At least 50% of the substrate particles have a particle size of at least 1 mm. A system for the propagation of biological cells and a process for the propagation of biological cells are also described.

Abstract: A comprehensive reconstruction method for long-series sediment data in data-lacking areas includes steps of: collecting hydrological and sediment data of a target river section; calculating sediment data in data-rich years with a flow-sediment content annual relationship curve method; calculating sediment data in only water quality and sediment test years with a correlation method between water quality and sediment data and hydrological station sediment data; calculating sediment data in data-lacking years with an adjacent station same year flow-sediment content relationship curve method; and calculating sediment data in remaining years with a multi-year average flow-sediment content relationship curve method.

Abstract: A method of constructing a surgical implant is provided. The method comprises providing a multiplicity of substantially identical blocks, each having features thereon to allow blocks to be mechanically joined together. A surgical implant may then be constructed by using these features to join the blocks together into a desired configuration.

Abstract: A method of constructing a surgical implant is provided. The method comprises providing a multiplicity of substantially identical blocks, each having features thereon to allow blocks to be mechanically joined together. A surgical implant may then be constructed by using these features to join the blocks together into a desired configuration.

Abstract: A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.

Abstract: The present invention relates to a membrane separation method and a relevant equipment, in particular to a method and an equipment for membrane separation utilizing concentration polarization during membrane filtration process, especially, to a concentration process and equipment and a drawer special for drawing a concentration polarization layer. The direct removal of the concentration polarization layer from membrane surface not only decreases the adverse influence of concentration polarization on membrane separation but also obtains concentrated retention components, thereby significantly improving the ability to maintain membrane flux, solving the twinborn problems concerning concentration polarization and membrane fouling during the membrane separation process, and achieving a high-efficiency concentration for retention components.

Abstract: The present invention relates to a membrane separation method and a relevant equipment, in particular to a method and an equipment for membrane separation utilizing concentration polarization during membrane filtration process, especially, to a concentration process and equipment and a drawer special for drawing a concentration polarization layer. The direct removal of the concentration polarization layer from membrane surface not only decreases the adverse influence of concentration polarization on membrane separation but also obtains concentrated retention components, thereby significantly improving the ability to maintain membrane flux, solving the twinborn problems concerning concentration polarization and membrane fouling during the membrane separation process, and achieving a high-efficiency concentration for retention components.

Abstract: A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.

Abstract: The present invention relates to a membrane separation method and a relevant equipment, in particular to a method and an equipment for membrane separation utilizing concentration polarization during membrane filtration process, especially, to a concentration process and equipment and a drawer special for drawing a concentration polarization layer. The direct removal of the concentration polarization layer from membrane surface not only decreases the adverse influence of concentration polarization on membrane separation but also obtains concentrated retention components, thereby significantly improving the ability to maintain membrane flux, solving the twinborn problems concerning concentration polarization and membrane fouling during the membrane separation process, and achieving a high-efficiency concentration for retention components.

Abstract: A method of producing an in vitro microtumour comprising: seeding a colorectal neoplastic cell into a three dimensional scaffold comprising polysaccharide co-polymer; providing said cell with a culture medium that supports the growth thereof; and incubating said cell in said scaffold for a time sufficient for microtumors to form, wherein said polysaccharide copolymer comprises glutaronate and mannuronate.

Abstract: A gravity driven portable water purification device (1) comprises a dirty water inlet (5) operative to comprise a head of dirty water, water filtration means (23) connected to the dirty water inlet (5), and a clean water tank (35) connected to an outlet of the water filtration means (23). The dirty water inlet (5), when the device (1) is in an operational condition, is arranged relative to the clean water tank (35) and the water filtration means (23) such that, in use of the device, dirty water is forced through the water filtration means (23) and into the clean water tank (35) by the gravitational force generated from the head of dirty water in the dirty water inlet (5). The water filtration means (23) comprises a cross-flow membrane filter in which the gravitational force from the head of dirty water also forces dirty water across the cross-flow membrane filter so as to force contaminants out of the filter.

Abstract: The present invention relates to a membrane separation method and a relevant equipment, in particular to a method and an equipment for membrane separation utilizing concentration polarization during membrane filtration process, especially, to a concentration process and equipment and a drawer special for drawing a concentration polarization layer. The direct removal of the concentration polarization layer from membrane surface not only decreases the adverse influence of concentration polarization on membrane separation but also obtains concentrated retention components, thereby significantly improving the ability to maintain membrane flux, solving the twinborn problems concerning concentration polarization and membrane fouling during the membrane separation process, and achieving a high-efficiency concentration for retention components.

Abstract: A cell culture plate for perfused cell or tissue culture comprises one or more wells. The wells are formed from a biocompatible gas permeable polymer. Means are provided to perfuse each well.