Abstract: Embodiments of the disclosure relate to methods for metal gapfill of a logic device with lower resistivity. Specific embodiments provide integrated separate tungsten PVD processes with plasma-etch to solve the overhang issue caused by tungsten PVD and the high resistivity caused by nucleation.

Abstract: The present invention relates to lysosomal enzymes modified by use of cell based methods, a compositions comprising a modified lysosomal enzyme, as well as methods for producing a modified lysosomal enzyme and therapeutic use of such modified lysosomal enzyme. In particular, the present disclosure relates to a modified lysosomal enzyme which has low Man6P and low exposed Mannose and high sialic acid content of alpha2,3 type enabling long circulation time and improved uptake into difficult-to-reach organs like heart, kidney and brain.

Abstract: A method for inversion of a path mileage and offset by using a known coordinate point, comprising: based on coordinates of a known point P and curve elements of a start location and an end location of a curve element, first segmenting or not segmenting the curve element according to a corner of the curve element to obtain a plurality of curve sub-elements (AiBi), and calculating curve elements of the curve sub-elements (AiBi); taking any curve sub-element (AiBi) to calculate a half chord length S and a straight corner ?, and performing precision determination and convergence correction according to S and ?, the point P being always effective with respect to the curve sub-element (AiBi) in the correction process; and performing cyclic convergence calculation to finally obtain a distance from the point P to any point on a chord line or an arc line as an offset of the curve sub-element (AiBi) corresponding to the point P and to obtain a mileage of the curve sub-element (AiBi) corresponding to the point P.

Abstract: A casing structure includes a plurality of first position-limiting rod elements, a main casing element, a cover element and a first shielding element. The main casing element has an accommodating space. The cover element is detachably connected to the main casing element. The first shielding element is detachably connected to the main casing element or the cover element. When the casing structure is in a storage state, the first position-limiting rod elements are located in the accommodating space. Therefore, the casing structure provided by the present invention can improve convenience during transportation.

Abstract: A method for inversion of a path mileage and offset by using a known coordinate point, comprising: based on coordinates of a known point P and curve elements of a start location and an end location of a curve element, first segmenting or not segmenting the curve element according to a corner of the curve element to obtain a plurality of curve sub-elements (AiBi), and calculating curve elements of the curve sub-elements (AiBi); taking any curve sub-element (AiBi) to calculate a half chord length S and a straight corner ?, and performing precision determination and convergence correction according to S and ?, the point P being always effective with respect to the curve sub-element (AiBi) in the correction process; and performing cyclic convergence calculation to finally obtain a distance from the point P to any point on a chord line or an arc line as an offset of the curve sub-element (AiBi) corresponding to the point P and to obtain a mileage of the curve sub-element (AiBi) corresponding to the point P.

Abstract: The present invention relates to lysosomal enzymes modified by use of cell based methods, a compositions comprising a modified lysosomal enzyme, as well as methods for producing a modified lysosomal enzyme and therapeutic use of such modified lysosomal enzyme. In particular, the present disclosure relates to a modified lysosomal enzyme which has low Man6P and low exposed Mannose and high sialic acid content of alpha2,3 type enabling long circulation time and improved uptake into difficult-to-reach organs like heart, kidney and brain.

Abstract: A method of depositing a coating on a substrate comprises simultaneously depositing a first material via a CVA process and a second material via a sputtering process; also described are coatings obtained therefrom and coated substrates.

Abstract: The present invention relates to lysosomal enzymes modified by use of cell based methods, a compositions comprising a modified lysosomal enzyme, as well as methods for producing a modified lysosomal enzyme and therapeutic use of such modified lysosomal enzyme. In particular, the present disclosure relates to a modified lysosomal enzyme which has low Man6P and low exposed Mannose and high sialic acid content of alpha2,3 type enabling long circulation time and improved uptake into difficult-to-reach organs like heart, kidney and brain.

Abstract: One example includes a circuit that includes a transistor control circuit having an input and an output adapted to be coupled to the output of the transistor control circuit and can provide a slew-rate compensation voltage proportional to a slew-rate of a control voltage of the transistor. A reference voltage source can be coupled to the slew-rate compensator to provide a reference voltage at the output of the reference voltage source, the slew-rate compensator configured to add the slew-rate compensation voltage to the reference voltage to provide an adjusted reference voltage at the output of the slew rate compensator. A reference comparator having a first input, a second input and an output is coupled to the input of the transistor control circuit. The first input can be coupled to the control terminal of the transistor, and the second input can be coupled to the output of the slew-rate compensator.

Abstract: The present invention relates to a mammalian cell comprising a gene encoding a polypeptide of interest, wherein the polypeptide of interest is expressed comprising one or more posttranslational modification patterns. These modifications are useful for example in glycoprotein production where the antibodies with the modifications have an enhanced antibody-dependent cell-mediated cytotoxicity (ADCC). The present invention also relates to methods for producing the glycoproteins and compositions comprising the glycoproteins, and their uses.

Abstract: The present invention relates to a mammalian cell comprising a gene encoding a polypeptide of interest, wherein the polypeptide of interest is expressed comprising one or more posttranslational modification patterns. These modifications are useful for example in glycoprotein production where the antibodies with the modifications have an enhanced antibody-dependent cell-mediated cytotoxicity (ADCC). The present invention also relates to methods for producing the glycoproteins and compositions comprising the glycoproteins, and their uses.

Abstract: The present invention relates to a mammalian cell comprising a gene encoding a polypeptide of interest, wherein the polypeptide of interest is expressed comprising one or more posttranslational modification patterns. These modifications are useful for example in glycoprotein production where the antibodies with the modifications have an enhanced antibody-dependent cell-mediated cytotoxicity (ADCC). The present invention also relates to methods for producing the glycoproteins and compositions comprising the glycoproteins, and their uses.

Abstract: The present invention relates to a cell comprising a gene encoding a polypeptide of interest, wherein the polypeptide of interest is expressed comprising one or more posttranslational modification patterns. These modifications are useful for example in improvement of pharmacokinetic properties, i.e. by attaching PEG chains to proteins. The present invention also relates to methods for producing the antibodies and compositions comprising the antibodies, and their uses.

Abstract: The present invention relates to a mammalian cell comprising a gene encoding a polypeptide of interest, wherein the polypeptide of interest is expressed comprising one or more posttranslational modification patterns. These modifications are useful for example in glycoprotein production where the antibodies with the modifications have an enhanced antibody-dependent cell-mediated cytotoxicity (ADCC). The present invention also relates to methods for producing the glycoproteins and compositions comprising the glycoproteins, and their uses.

Abstract: The present invention relates to a method for display of a plurality of mammalian glycans on cells or proteins for probing biological interactions and identifying glycan structures involved. A plurality of mammalian cells is genetically engineered in a combinatorial approach to differentially express the human glycome. Genetic engineering of the cell produces a plurality isogenic cells with different repertoires of glycosyltransferases and display of glycans that is used to interpret biological interactions. The plurality of engineered cells display glycans with and without the context of specific proteins exogeneously expressed, and is useful for detection and optimization of biological interactions for example binding of lectins, antibodies, viruses and bacteria and glycoproteins.

Abstract: The present invention relates to a mammalian cell comprising a gene encoding a polypeptide of interest, wherein the polypeptide of interest is expressed comprising one or more posttranslational modification patterns. These modifications are useful for example in glycoprotein production where the antibodies with the modifications have an enhanced antibody-dependent cell-mediated cytotoxicity (ADCC). The present invention also relates to methods for producing the glycoproteins and compositions comprising the glycoproteins, and their uses.

Abstract: Disclosed herein is a file management system that includes an unformatted raw data area storing a plurality of raw data files at respective locations within the unformatted raw data area. The storage medium also includes a formatted partitioned area that includes a plurality of partitions each associated with a different file system. Each partition includes a plurality of metadata files each corresponding with one of the plurality of raw data files. Each metadata file includes metadata regarding the corresponding one of the plurality of raw data files.

Abstract: Disclosed herein is a file management system that includes an unformatted raw data area storing a plurality of raw data files at respective locations within the unformatted raw data area. The storage medium also includes a formatted partitioned area that includes a plurality of partitions each associated with a different file system. Each partition includes a plurality of metadata files each corresponding with one of the plurality of raw data files. Each metadata file includes metadata regarding the corresponding one of the plurality of raw data files.

Abstract: This invention discloses the development of a novel platform for recombinant production of bioactive glycoproteins and cancer specific vaccines in plants. Plants and plant cell cultures have been humanized with respect to human mucin-type protein O-glycosylation. A panel of plant cell factories for production of recombinant glycoproteins with designed human O-glycosylation, including an improved cancer vaccine candidate, has been developed. The platform provides basis for i) production of an essentially unlimited array of O-glycosylated human glycoprotein therapeutics, such as human interferon ?2B and podoplanin, and ii) for further engineering of additional cancer specific O-glycans on glycoproteins of therapeutical value. Currently, mammalian cells are required for human O-glycosylation, but plants offer a unique cell platform for engineering O-glycosylation since they do not perform human type O-glycosylation.

Abstract: Methods of forming isolation structures are disclosed. A method of forming isolation structures for an image sensor array of one aspect may include forming a dielectric layer over a semiconductor substrate. Narrow, tall dielectric isolation structures may be formed from the dielectric layer. The narrow, tall dielectric isolation structures may have a width that is no more than 0.3 micrometers and a height that is at least 1.5 micrometers. A semiconductor material may be epitaxially grown around the narrow, tall dielectric isolation structures. Other methods and apparatus are also disclosed.