Abstract: Provided is a method for biosynthesis of (R)-?-hydroxytetradecanoate compounds. The method comprises the following steps of: reacting ?-carbonyl tetradecanoate in a ketoreductase and a glucose dehydrogenase or a fusion enzyme thereof, glucose, NADP+ and a buffer solution to obtain (R)-?-hydroxytetradecanoate compounds. The method is an enzyme-catalyzed biosynthesis method having simple operations, conventional equipment and an environmentally friendly process, and the prepared product has high purity, high yield and a high ee value.