Abstract: Disclosed are a heterocyclic compound, and an intermediate thereof, a preparation method therefor and the use thereof. Namely, provided are a heterocyclic compound as shown in formula I-a, and a tautomer and a pharmaceutically acceptable salt thereof, wherein the compound has a good water solubility and pharmacokinetic properties.

Abstract: Disclosed herein are methods of detecting sepsis in a patient in need thereof, the method comprising: receiving a patient sample, a pathogenic primer mix, a fluorophore label probe, and a dPCR buffer in one or more wells of a microfluidic device and detecting fluorescent light signal from the droplets to determine sepsis in the patient. The microfluidic device comprises a droplet generation channel in fluid communication with the one or more wells, adapted to generate droplets comprising the patient sample, one or more pathogenic primers, a fluorophore label probe, and dPCR buffer. Furthermore, the microfluidic device comprises a chamber in fluid communication with the droplet generation channel for collecting the droplets generated by the droplet generation channel and performing a PCR reaction in the droplets.