Patents by Inventor Zhaochun Ma

Zhaochun Ma has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHODS, COMPOSITIONS, SYSTEMS, APPARATUSES AND KITS FOR NUCLEIC ACID AMPLIFICATION
    Publication number: 20230175051
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Application
    Filed: February 1, 2023
    Publication date: June 8, 2023
    Inventors: Bin Li, Kai Qin LAO, Jennifer O'NEIL, Jennifer KUNKEL, Kellie HALEY, Rachel KASINSKAS, Zhaochun MA, Pius BRZOSKA
  • Method of amplifying a target nucleic acid
    Patent number: 11655497
    Abstract: The present disclosure provides a method of amplifying a target nucleic acid, wherein the method comprises: (a) providing a reaction mixture comprising: (i) a nucleic acid sample comprising or suspected of comprising the target nucleic acid, (ii) multiple primer pairs, wherein at least one primer of each type of primer pairs is complementary to a portion of the target nucleic acid, and each primer pair has at least one blocking primer comprising a blocking group capable of blocking polymerase extension, (iii) nucleic acid polymerase, and (iv) de-blocking agent capable of enabling polymerization of the target nucleic acid by said nucleic acid polymerase using the blocking primers; and (b) incubating the reaction mixture under a condition for amplification of the target nucleic acid and a kit used for the method. The present disclosure further provides a method of sequencing a target nucleic acid and a kit used for the method.
    Type: Grant
    Filed: September 19, 2018
    Date of Patent: May 23, 2023
    Assignee: NINGBO SHINING BIOTECHNOLOGY CO., LTD
    Inventor: Zhaochun Ma
  • Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
    Patent number: 11578360
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Grant
    Filed: October 13, 2020
    Date of Patent: February 14, 2023
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Bin Li, Kai Qin Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska
  • A METHOD OF AMPLIFYING A TARGET NUCLEIC ACID
    Publication number: 20210189477
    Abstract: The present disclosure provides a method of amplifying a target nucleic acid, wherein the method comprises: (a) providing a reaction mixture comprising: (i) a nucleic acid sample comprising or suspected of comprising the target nucleic acid, (ii) multiple primer pairs, wherein at least one primer of each type of primer pairs is complementary to a portion of the target nucleic acid, and each primer pair has at least one blocking primer comprising a blocking group capable of blocking polymerase extension, (iii) nucleic acid polymerase, and (iv) de-blocking agent capable of enabling polymerization of the target nucleic acid by said nucleic acid polymerase using the blocking primers; and (b) incubating the reaction mixture under a condition for amplification of the target nucleic acid and a kit used for the method. The present disclosure further provides a method of sequencing a target nucleic acid and a kit used for the method.
    Type: Application
    Filed: September 19, 2018
    Publication date: June 24, 2021
    Inventor: Zhaochun MA
  • Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
    Patent number: 10913976
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Grant
    Filed: March 12, 2019
    Date of Patent: February 9, 2021
    Assignee: Life Technologies Corporation
    Inventors: Bin Li, Kai Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska
  • METHODS, COMPOSITIONS, SYSTEMS, APPARATUSES AND KITS FOR NUCLEIC ACID AMPLIFICATION
    Publication number: 20210032691
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Application
    Filed: October 13, 2020
    Publication date: February 4, 2021
    Inventors: Bin LI, Kai Qin LAO, Jennifer O'NEIL, Jennifer KUNKEL, Kellie HALEY, Rachel KASINSKAS, Zhaochun MA, Pius BRZOSKA
  • METHODS, COMPOSITIONS, SYSTEMS, APPARATUSES AND KITS FOR NUCLEIC ACID AMPLIFICATION
    Publication number: 20190264272
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Application
    Filed: March 12, 2019
    Publication date: August 29, 2019
    Inventors: Bin LI, Kai Lao, Jennifer O'NEIL, Jennifer KUNKEL, Kellie HALEY, Rachel KASINSKAS, Zhaochun MA, Pius BRZOSKA
  • Clonal amplification of nucleic acid on solid surface with template walking
    Patent number: 10233488
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Grant
    Filed: September 26, 2016
    Date of Patent: March 19, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Bin Li, Kai Qin Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska
  • SYNTHESIS OF 2', 3'-DIDEOXYNUCLEOSIDES FOR AUTOMATED DNA SYNTHESIS AND PYROPHOSPHOROLYSIS ACTIVATED POLYMERIZATION
    Publication number: 20170305954
    Abstract: Methods for preparation of 2?,3?-dideoxynucleotides support structures, such as 2?,3?-dideoxyguanosine, 2?,3?-dideoxyadenosine, and 3?-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
    Type: Application
    Filed: July 6, 2017
    Publication date: October 26, 2017
    Applicant: Life Technologies Corporation
    Inventors: Zhaochun Ma, Khairuzzaman Bashar Mullah, Robert G. Eason
  • Synthesis of 2?,3?-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization
    Patent number: 9732112
    Abstract: Methods for preparation of 2?,3?-dideoxynucleotides support structures, such as 2?,3?-dideoxyguanosine, 2?,3?-dideoxyadenosine, and 3?-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
    Type: Grant
    Filed: November 25, 2015
    Date of Patent: August 15, 2017
    Assignee: Life Technologies Corporation
    Inventors: Zhaochun Ma, Khairuzzaman Bashar Mullah, Robert G. Eason
  • POLYMERIZATION OF NUCLEIC ACIDS USING ACTIVATION BY POLYPHOSPHOROLYSIS (APP) REACTIONS
    Publication number: 20170081706
    Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or ?-monothiopyrophosphate), and related compounds.
    Type: Application
    Filed: September 30, 2016
    Publication date: March 23, 2017
    Inventors: Lei XI, Paul KENNEY, Zhaochun MA, Dennis PROSEN, Stephen HENDRICKS, Roland NAGEL
  • CLONAL AMPLIFICATION OF NUCLEIC ACID ON SOLID SURFACE WITH TEMPLATE WALKING
    Publication number: 20170067098
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Application
    Filed: September 26, 2016
    Publication date: March 9, 2017
    Inventors: Bin LI, Kai Qin LAO, Jennifer O'NEIL, Jennifer KUNKEL, Kellie HALEY, Rachel KASINSKAS, Zhaochun MA, Pius BRZOSKA
  • Use of antibody-surrogate antigen systems for detection of analytes
    Patent number: 9575069
    Abstract: Compositions, methods and kits for labeling proteins and uses in reporter systems for detecting, quantifying and/or characterizing analytes.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: February 21, 2017
    Assignee: APPLIED BIOSYSTEMS, LLC
    Inventors: Steven Menchen, Joe Y. Lam, Zhaochun Ma, Khairuzzaman Bashar Mullah
  • Polymerization of nucleic acids using activation by polyphosphorolysis (APP) reactions
    Patent number: 9476093
    Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or ?-monothiopyrophosphate), and related compounds.
    Type: Grant
    Filed: January 9, 2015
    Date of Patent: October 25, 2016
    Assignee: Life Technologies Corporation
    Inventors: Lei Xi, Paul Kenney, Zhaochun Ma, Dennis Prosen, Stephen Hendricks, Roland Nagel
  • Clonal amplification of nucleic acid on solid surface with template walking
    Patent number: 9476080
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Grant
    Filed: April 21, 2015
    Date of Patent: October 25, 2016
    Assignee: Life Technologies Corporation
    Inventors: Bin Li, Kai Qin Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska
  • Synthesis Of 2',3'-Dideoxynucleosides For Automated DNA Synthesis And Pyrophosphorolysis Activated Polymerization
    Publication number: 20160108079
    Abstract: Methods for preparation of 2?,3?-dideoxynucleotides support structures, such as 2?,3?-dideoxyguanosine, 2?,3?-dideoxyadenosine, and 3?-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
    Type: Application
    Filed: November 25, 2015
    Publication date: April 21, 2016
    Inventors: Zhaochun MA, Khairuzzaman Bashar MULLAH, Robert G. EASON
  • Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
    Patent number: 9309566
    Abstract: In some embodiments, the present teachings provide methods for paired end sequencing. In some embodiment, a polynucleotide template to be subjected to paired end sequencing comprises at least one cross linking moiety and at least one scissile moiety. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cleavage step, and (c) a reverse sequencing step. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cross-linking step, (c) a cleavage step, and (d) a reverse sequencing step.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: April 12, 2016
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Bin Li, Kai Qin Lao, Jennifer O'Neil, Jennifer Kunkel, Kellie Haley, Rachel Kasinskas, Zhaochun Ma, Pius Brzoska
  • Synthesis of 2?, 3?-dideoxynucleosides for automated DNA synthesis and pyrophosphorolysis activated polymerization
    Patent number: 9228227
    Abstract: Methods for preparation of 2?,3?-dideoxynucleotides support structures, such as 2?,3?-dideoxyguanosine, 2?,3?-dideoxyadenosine, and 3?-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
    Type: Grant
    Filed: February 5, 2014
    Date of Patent: January 5, 2016
    Assignee: Life Technologies Corporation
    Inventors: Zhaochun Ma, Khairuzzaman Bashar Mullah, Robert Eason
  • CLONAL AMPLIFICATION OF NUCLEIC ACID ON SOLID SURFACE WITH TEMPLATE WALKING
    Publication number: 20150275284
    Abstract: Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.
    Type: Application
    Filed: April 21, 2015
    Publication date: October 1, 2015
    Inventors: Bin LI, Kai Qin LAO, Jennifer O'NEIL, Jennifer KUNKEL, Kellie HALEY, Rachel KASINSKAS, Zhaochun MA, Pius BRZOSKA
  • USE OF ANTIBODY-SURROGATE ANTIGEN SYSTEMS FOR DETECTION OF ANALYTES
    Publication number: 20150198605
    Abstract: Compositions, methods and kits for labeling proteins and uses in reporter systems for detecting, quantifying and/or characterizing analytes.
    Type: Application
    Filed: November 8, 2013
    Publication date: July 16, 2015
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Steven MENCHEN, Joe Y. Lam, Zhaochun Ma, Khairuzzaman Bashar Mullah