Abstract: The present disclosure provides a tea plant CsVAAT3 gene and use thereof. The tea plant CsVAAT3 gene has a nucleotide sequence shown in SEQ ID NO: 1 in sequence listing. A protein coded by the tea plant CsVAAT3 gene has an amino acid sequence shown in SEQ ID NO: 2 in the sequence listing. An expression pattern of CsVAAT3 in tea plant is significantly positively correlated with the nitrogen level in tea roots. The CsVAAT3 is highly expressed in tea roots. A pDR196-CsVAAT3 plasmid constructed from this gene is transformed into a vacuolar amino acid uptake-deficient yeast strain ypq2, which can restore the growth ability of the yeast mutant on a high-concentration theanine medium. Cloning of the gene is beneficial to analysis of a molecular mechanism of theanine storage in tea roots, providing an important target gene resource and a theoretical basis for cultivation of new tea cultivars with high theanine content.

Abstract: The present disclosure provides a tea plant CsFAAH6 gene and use thereof. The tea plant CsFAAH6 gene has a nucleotide sequence shown in SEQ ID NO: 1 in sequence listing. A protein coded by the tea plant CsFAAH6 gene has an amino acid sequence shown in SEQ ID NO: 2 in the sequence listing. The CsFAAH6 is highly expressed in mature leaves of tea plants; there is significant negative correlation between theanine content in shoots (one bud and two leaves) of different tea cultivars in different months and expression level of the CsFAAH6; instantaneous silencing of CsFAAH6 expression can significantly increase the theanine content, indicating that CsFAAH6 has a physiological function of theanine degradation and a molecular mechanism thereof.