Abstract: The present disclosure provides a combination of antisense RNA sequences and use thereof in the production of tilapia with degenerated sexual organs, belonging to the technical field of molecular biology and reproductive biology, the combination of antisense RNA sequences includes antisense RNA of steroidogenic factors SF1-1 and SF1-2; the nucleotide sequences of Anti-SF1-1-I, Anti-SF1-1-II, Anti-SF1-2-I and Anti-SF1-2-II are set forth in SEQ ID NO:1-SEQ ID NO:4 respectively. The method of the present disclosure introduces antisense RNA fragments into the eggs through the fertilization hole to realize effective and accurate targeted intervention for regulating the gene expression, and the method has the advantages of simple operation, minimal egg damage, high success rate, stable phenotype after breeding, and excellent application prospects.

Abstract: The present disclosure provides a combination of antisense RNA sequences and use thereof in the production of abortive tilapia, belonging to the technical field of molecular biology and reproductive biology, the combination of antisense RNA sequences includes antisense RNA of steroidgenic factors SF1-1 and SF1-2; the nucleotide sequences of Anti-SF1-1-I, Anti-SF1-1-II, Anti-SF1-2-I and Anti-SF1-2-II are set forth in SEQ ID NO. 1-SEQ ID NO. 4 respectively. The method of the present disclosure introduces antisense RNA fragments into the eggs through the fertilization hole to realize effective and accurate targeted intervention for regulating the gene expression, and the method has the advantages of simple operation, minimal egg damage, high success rate, stable phenotype after breeding, and excellent application prospects.

Abstract: The present application provides a solution for prolonging open time of fertilization hole of fish eggs and a method for introducing exogenous gene through fertilization hole, belonging to the technical field of research and production of transgenic fish, wherein the solution takes water as a solvent and includes 6 g of sodium chloride, 0.15 g of potassium chloride, 0.1 g of calcium chloride, 0.1 g of sodium bicarbonate, 0.1 g of sodium dihydrogen phosphate and 1 g of glucose per liter. The solution provided by the present application can prolong the open time of the fertilization hole of the fish eggs, so that the exogenous genes can enter the fish eggs directly.