Abstract: We hereby disclose and claim a mathematically based system for design and implementation synthetic biology genetic circuitry architecture which uses genetic expression circuits which are linked together to achieve a therapeutic or diagnostic effect. The genetic architecture that enables the reversible and tunable desired gene product output is achieved by genetic control elements upstream and downstream of a promoter region. Reversible and tunable control is achieved through the computational design and pairing of transcriptional repression elements with their cognate transcriptional promoters to enable a broad range of therapeutic and diagnostic applications.

Abstract: Provided is a modular construction of synthetic gene circuits in mammalian cells using TALE transcriptional repressors.

Abstract: An expression system, comprising: a first nucleic acid molecule having a cell-specific promoter; a second nucleic acid molecule which encodes a transcriptional activator; a third nucleic acid molecule having a first recognition sequence of the transcriptional activator; a fourth nucleic acid molecule having a first promoter and a first regulatory element; a fifth nucleic acid molecule which encodes a first regulatory protein; a sixth nucleic acid molecule having a second recognition sequence of the transcriptional activator; a seventh nucleic acid molecule having a second promoter and a second regulatory element; an eighth nucleic acid molecule which encodes a second regulatory protein; as well as a ninth nucleic acid molecule which is configured to conditionally inhibit the expression of the first regulatory protein, and a tenth nucleic acid molecule which is configured to conditionally inhibit the expression of the second regulatory protein; the first regulatory element is adapted to inhibit the function of

Abstract: A risk analysis system configures the decision engine to detect anomalous online activities by analyzing usage patterns associated with one or more user accounts across multiple frequencies. The risk analysis system obtains transaction log data representing transactions associated with one or more accounts, and extracts data from the transaction log data to generate time-series data along a time dimension. The time-series data may represent usage characteristics of one or more user accounts over a period of time. The risk analysis system derives pattern data representing usage patterns across multiple different frequencies based on the time-series data. The risk analysis system then configures the decision engine to detect anomalous account activities based on the derived pattern data.

Abstract: The presently claimed invention offers programmable and precise regulation of Cas9 functions by utilizing a set of compact Cas9 derivatives created by deleting conserved HNH and/or REC-C domains based on the structural information across variant class 2 CRISPR effectors. In addition, a novel strategy for engineering the dimeric gRNA-guided nuclease by splitting the mini-dSaCas9 and fusing the FokI domain right after the split point is claimed to increase the on-target DNA cleavage efficiency and potentially reduce the off-target effect because of a closer proximity of dimeric FokI nuclease to the target sequence. By combining the optimized and compact gRNA expression cassette and the downsized SaCas9 derivatives, the entire CRISPR/Cas system with different effector domains for transactivation, DNA cleavage and base editing is loaded into a single AAV virus. Such an all-in-one AAV-CRISPR/Cas9 system will be particularly appealing in biomedical applications that require safe and efficient delivery in vivo.

Abstract: The present disclosure provides a lighting device and related voice broadcasting systems and methods. The lighting device includes a light-emitting module configured to provide lighting, a process and control module, a memory module configured to store audio contents stored in a local memory and downloaded from the Internet, and a voice input module configured to receive voice information from users. The process and control module is configured to receive voice information from the voice input module, to recognize and determine whether the voice information is a voice command, and to send control instructions to a corresponding module according to the matched voice command. A voice output module is configured to select and play a audio content according to the control instructions sent from the process and control module.

Abstract: Devices, systems and methods are disclosed for enabling a device to act as a wireless extender and adaptively control a transmission power for capability messages. For example, when the device has the capability to relay traffic without affecting a local processing of the device, the device may transmit capability messages at a normal transmission power and may relay traffic between a network device and clients. However, when the device no longer has the capability to relay traffic without affecting the local processing, the device may transmit capability messages at a reduced transmission power. As clients use the capability messages to determine a signal strength of an access point, the reduced transmission power results in the device appearing to have a lower signal strength and thus decreases a desirability of the device as an access point.

Abstract: Methods, systems, devices, and apparatuses are described for wireless communications in which first type of traffic may be transmitted from a gateway access point (AP) directly to a station. Beacon signals transmitted to the station are transmitted as part of the first type of traffic. A second type of traffic may be transmitted from the gateway AP to the station via at least one relay AP. The first type of traffic may include low-throughput traffic and may be transmitted over a long-range radio link (e.g., 2 GHz band link or sub-1 GHz band link). The second type of traffic may include high-throughput traffic and may be transmitted over at least one short-range radio link (e.g., 5 GHz band link). The gateway AP may receive low-throughput traffic directly from the station and high-throughput traffic from the station via the at least one relay AP.

Abstract: While genetic engineering has undergone rapid advancement with the discovery of CRISPR/Cas9, there is room for improvement for genetic circuit control, precision (reducing circuit ‘leakiness’) and delivery into living systems. The claimed invention offers programmable and precise regulation of dCas9 functions in response to multiple molecular signals by using synthetic gene circuits, greatly expanding applications. Moreover, using the system to greatest therapeutic potential has been greatly limited by the restrictive cargo size of existing viral delivery systems. By splitting dCas9 into multiple sections, the delivery size of synthetic gene circuits is greatly reduced. By exchanging split dCas9 domains, differential regulation on one gene, or activating two different genes in response to cell-type specific microRNAs is illustrated.

Abstract: Provided is a modular construction of synthetic gene circuits in mammalian cells using TALE transcriptional repressors.

Abstract: Provided herein, in some embodiments, are modular transcriptional architectures and methods for regulated expression of guide RNAs in cells, such as human cells, which are based on Clustered Regularly Interspaced Palindromic Repeats (CRISPR) systems.

Abstract: Provided herein are high-input detector modules and multi-input biological classifier circuits and systems that integrate sophisticated sensing, information processing, and actuation in living cells and permit new directions in basic biology, biotechnology and medicine. The multi-input biological classifier circuits described herein comprise synthetic, scaleable transcriptional/post-transcriptional regulatory circuits that are designed to interrogate the status of a cell by simultaneously sensing expression levels of multiple endogenous inputs, such as microRNAs. The classifier circuits then compute whether to trigger a desired output or response if the expression levels match a pre-determined profile of interest.

Abstract: The present disclosure provides a lighting device and related voice broadcasting systems and methods. The lighting device includes a light-emitting module configured to provide lighting, a process and control module, a memory module configured to store audio contents stored in a local memory and downloaded from the Internet, and a voice input module configured to receive voice information from users. The process and control module is configured to receive voice information from the voice input module, to recognize and determine whether the voice information is a voice command, and to send control instructions to a corresponding module according to the matched voice command. A voice output module is configured to select and play a audio content according to the control instructions sent from the process and control module.

Abstract: Methods, systems, and devices are described for beacon based time division multiplexing (TDM) synchronization for multiple radio access technology (RAT) coexistence. In some aspects, TDM time slots corresponding to a plurality of RATs for wireless communication among a plurality of devices may be identified, and the TDM time slots may be synchronized based at least in part on a timing beacon associated with a first of the plurality of RATs.

Abstract: Methods, systems, devices, and apparatuses are described for wireless communications in which first type of traffic may be transmitted from a gateway access point (AP) directly to a station. Beacon signals transmitted to the station are transmitted as part of the first type of traffic. A second type of traffic may be transmitted from the gateway AP to the station via at least one relay AP. The first type of traffic may include low-throughput traffic and may be transmitted over a long-range radio link (e.g., 2 GHz band link or sub-1 GHz band link). The second type of traffic may include high-throughput traffic and may be transmitted over at least one short-range radio link (e.g., 5 GHz band link). The gateway AP may receive low-throughput traffic directly from the station and high-throughput traffic from the station via the at least one relay AP.

Abstract: Provided herein are high-input detector modules and multi-input biological classifier circuits and systems that integrate sophisticated sensing, information processing, and actuation in living cells and permit new directions in basic biology, biotechnology and medicine. The multi-input biological classifier circuits described herein comprise synthetic, scaleable transcriptional/post-transcriptional regulatory circuits that are designed to interrogate the status of a cell by simultaneously sensing expression levels of multiple endogenous inputs, such as microRNAs. The classifier circuits then compute whether to trigger a desired output or response if the expression levels match a pre-determined profile of interest.

Abstract: Disclosed herein are autonomous molecular circuits that can function in cells. The circuits can process logical operations in which one or more input cues are among the operands and produce an appropriate output. Such circuits can be implemented in living cells, e.g., eukaryotic or prokaryotic cells that have been modified to include circuit components. The molecular circuits and cells containing the circuits can be used in a variety of applications including, e.g., diagnostics, therapeutics, and protein production.