Patents by Inventor Zhen Xie

Zhen Xie has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10563207
    Abstract: Provided is a modular construction of synthetic gene circuits in mammalian cells using TALE transcriptional repressors.
    Type: Grant
    Filed: March 6, 2015
    Date of Patent: February 18, 2020
    Inventors: Zhen Xie, Ron Weiss, Yinqing Li, Yun Jiang, Weixi Liao, He Chen
  • Publication number: 20200017881
    Abstract: An expression system, comprising: a first nucleic acid molecule having a cell-specific promoter; a second nucleic acid molecule which encodes a transcriptional activator; a third nucleic acid molecule having a first recognition sequence of the transcriptional activator; a fourth nucleic acid molecule having a first promoter and a first regulatory element; a fifth nucleic acid molecule which encodes a first regulatory protein; a sixth nucleic acid molecule having a second recognition sequence of the transcriptional activator; a seventh nucleic acid molecule having a second promoter and a second regulatory element; an eighth nucleic acid molecule which encodes a second regulatory protein; as well as a ninth nucleic acid molecule which is configured to conditionally inhibit the expression of the first regulatory protein, and a tenth nucleic acid molecule which is configured to conditionally inhibit the expression of the second regulatory protein; the first regulatory element is adapted to inhibit the function of
    Type: Application
    Filed: August 4, 2017
    Publication date: January 16, 2020
    Applicants: Beijing Syngentech Co., Ltd., Tsinghua University
    Inventors: Zhen Xie, Huiya Huang, Yiqi Liu, Weixi Liao
  • Publication number: 20200007564
    Abstract: A risk analysis system configures the decision engine to detect anomalous online activities by analyzing usage patterns associated with one or more user accounts across multiple frequencies. The risk analysis system obtains transaction log data representing transactions associated with one or more accounts, and extracts data from the transaction log data to generate time-series data along a time dimension. The time-series data may represent usage characteristics of one or more user accounts over a period of time. The risk analysis system derives pattern data representing usage patterns across multiple different frequencies based on the time-series data. The risk analysis system then configures the decision engine to detect anomalous account activities based on the derived pattern data.
    Type: Application
    Filed: July 2, 2018
    Publication date: January 2, 2020
    Inventors: Zhen Xie, Kasra Vakilinia, Yang Chen, Hagar Oppenheim, Xing Ji
  • Publication number: 20180163188
    Abstract: The presently claimed invention offers programmable and precise regulation of Cas9 functions by utilizing a set of compact Cas9 derivatives created by deleting conserved HNH and/or REC-C domains based on the structural information across variant class 2 CRISPR effectors. In addition, a novel strategy for engineering the dimeric gRNA-guided nuclease by splitting the mini-dSaCas9 and fusing the FokI domain right after the split point is claimed to increase the on-target DNA cleavage efficiency and potentially reduce the off-target effect because of a closer proximity of dimeric FokI nuclease to the target sequence. By combining the optimized and compact gRNA expression cassette and the downsized SaCas9 derivatives, the entire CRISPR/Cas system with different effector domains for transactivation, DNA cleavage and base editing is loaded into a single AAV virus. Such an all-in-one AAV-CRISPR/Cas9 system will be particularly appealing in biomedical applications that require safe and efficient delivery in vivo.
    Type: Application
    Filed: June 11, 2017
    Publication date: June 14, 2018
    Applicant: Tsinghua University
    Inventors: Zhen Xie, Dacheng Ma
  • Patent number: 9990175
    Abstract: The present disclosure provides a lighting device and related voice broadcasting systems and methods. The lighting device includes a light-emitting module configured to provide lighting, a process and control module, a memory module configured to store audio contents stored in a local memory and downloaded from the Internet, and a voice input module configured to receive voice information from users. The process and control module is configured to receive voice information from the voice input module, to recognize and determine whether the voice information is a voice command, and to send control instructions to a corresponding module according to the matched voice command. A voice output module is configured to select and play a audio content according to the control instructions sent from the process and control module.
    Type: Grant
    Filed: April 28, 2015
    Date of Patent: June 5, 2018
    Assignee: ZHEJIANG SHENGHUI LIGHTING CO., LTD
    Inventors: Zonggen Zhang, Shuyu Cao, Zhen Xie, Weisheng Zhou, Jinxiang Shen
  • Patent number: 9819901
    Abstract: Devices, systems and methods are disclosed for enabling a device to act as a wireless extender and adaptively control a transmission power for capability messages. For example, when the device has the capability to relay traffic without affecting a local processing of the device, the device may transmit capability messages at a normal transmission power and may relay traffic between a network device and clients. However, when the device no longer has the capability to relay traffic without affecting the local processing, the device may transmit capability messages at a reduced transmission power. As clients use the capability messages to determine a signal strength of an access point, the reduced transmission power results in the device appearing to have a lower signal strength and thus decreases a desirability of the device as an access point.
    Type: Grant
    Filed: May 6, 2015
    Date of Patent: November 14, 2017
    Assignee: AMAZON TECHNOLOGIES, INC.
    Inventors: Yi Zhu, Zhen Xie, Jin Guo
  • Patent number: 9749874
    Abstract: Methods, systems, devices, and apparatuses are described for wireless communications in which first type of traffic may be transmitted from a gateway access point (AP) directly to a station. Beacon signals transmitted to the station are transmitted as part of the first type of traffic. A second type of traffic may be transmitted from the gateway AP to the station via at least one relay AP. The first type of traffic may include low-throughput traffic and may be transmitted over a long-range radio link (e.g., 2 GHz band link or sub-1 GHz band link). The second type of traffic may include high-throughput traffic and may be transmitted over at least one short-range radio link (e.g., 5 GHz band link). The gateway AP may receive low-throughput traffic directly from the station and high-throughput traffic from the station via the at least one relay AP.
    Type: Grant
    Filed: July 17, 2013
    Date of Patent: August 29, 2017
    Assignee: QUALCOMM Incorporated
    Inventors: Shu Du, Ning Zhang, Zhen Xie, James Simon Cho, Chin-Hung Chen, Kai Shi
  • Publication number: 20170233703
    Abstract: While genetic engineering has undergone rapid advancement with the discovery of CRISPR/Cas9, there is room for improvement for genetic circuit control, precision (reducing circuit ‘leakiness’) and delivery into living systems. The claimed invention offers programmable and precise regulation of dCas9 functions in response to multiple molecular signals by using synthetic gene circuits, greatly expanding applications. Moreover, using the system to greatest therapeutic potential has been greatly limited by the restrictive cargo size of existing viral delivery systems. By splitting dCas9 into multiple sections, the delivery size of synthetic gene circuits is greatly reduced. By exchanging split dCas9 domains, differential regulation on one gene, or activating two different genes in response to cell-type specific microRNAs is illustrated.
    Type: Application
    Filed: December 12, 2016
    Publication date: August 17, 2017
    Applicant: Tsinghua University
    Inventors: Zhen Xie, Shuguang Peng, Dacheng Ma
  • Publication number: 20170145426
    Abstract: Provided is a modular construction of synthetic gene circuits in mammalian cells using TALE transcriptional repressors.
    Type: Application
    Filed: March 6, 2015
    Publication date: May 25, 2017
    Inventors: Zhen XIE, Ron WEISS, Yinqing LI, Yun JIANG, Weixi LIAO, He CHEN
  • Publication number: 20160326546
    Abstract: Provided herein, in some embodiments, are modular transcriptional architectures and methods for regulated expression of guide RNAs in cells, such as human cells, which are based on Clustered Regularly Interspaced Palindromic Repeats (CRISPR) systems.
    Type: Application
    Filed: May 4, 2016
    Publication date: November 10, 2016
    Applicants: Massachusetts Institute of Technology, Raytheon BBN Technologies, Corp.
    Inventors: Samira Kiani, Ron Weiss, Jacob S. Beal, Mohammad Reza Ebrahimkhani, Zhen Xie, Yinqing Li
  • Patent number: 9458509
    Abstract: Provided herein are high-input detector modules and multi-input biological classifier circuits and systems that integrate sophisticated sensing, information processing, and actuation in living cells and permit new directions in basic biology, biotechnology and medicine. The multi-input biological classifier circuits described herein comprise synthetic, scaleable transcriptional/post-transcriptional regulatory circuits that are designed to interrogate the status of a cell by simultaneously sensing expression levels of multiple endogenous inputs, such as microRNAs. The classifier circuits then compute whether to trigger a desired output or response if the expression levels match a pre-determined profile of interest.
    Type: Grant
    Filed: July 22, 2011
    Date of Patent: October 4, 2016
    Assignees: PRESIDENT AND FELLOWS OF HARVARD COLLEGE, MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Yaakov Benenson, Ron Weiss, Liliana Wroblewska, Zhen Xie
  • Publication number: 20160224315
    Abstract: The present disclosure provides a lighting device and related voice broadcasting systems and methods. The lighting device includes a light-emitting module configured to provide lighting, a process and control module, a memory module configured to store audio contents stored in a local memory and downloaded from the Internet, and a voice input module configured to receive voice information from users. The process and control module is configured to receive voice information from the voice input module, to recognize and determine whether the voice information is a voice command, and to send control instructions to a corresponding module according to the matched voice command. A voice output module is configured to select and play a audio content according to the control instructions sent from the process and control module.
    Type: Application
    Filed: April 28, 2015
    Publication date: August 4, 2016
    Inventors: ZONGGEN ZHANG, SHUYU CAO, ZHEN XIE, WEISHENG ZHOU, JINXIANG SHEN
  • Publication number: 20160100400
    Abstract: Methods, systems, and devices are described for beacon based time division multiplexing (TDM) synchronization for multiple radio access technology (RAT) coexistence. In some aspects, TDM time slots corresponding to a plurality of RATs for wireless communication among a plurality of devices may be identified, and the TDM time slots may be synchronized based at least in part on a timing beacon associated with a first of the plurality of RATs.
    Type: Application
    Filed: October 3, 2014
    Publication date: April 7, 2016
    Inventors: Jian Lu, Zhen Xie
  • Publication number: 20150023245
    Abstract: Methods, systems, devices, and apparatuses are described for wireless communications in which first type of traffic may be transmitted from a gateway access point (AP) directly to a station. Beacon signals transmitted to the station are transmitted as part of the first type of traffic. A second type of traffic may be transmitted from the gateway AP to the station via at least one relay AP. The first type of traffic may include low-throughput traffic and may be transmitted over a long-range radio link (e.g., 2 GHz band link or sub-1 GHz band link). The second type of traffic may include high-throughput traffic and may be transmitted over at least one short-range radio link (e.g., 5 GHz band link). The gateway AP may receive low-throughput traffic directly from the station and high-throughput traffic from the station via the at least one relay AP.
    Type: Application
    Filed: July 17, 2013
    Publication date: January 22, 2015
    Applicant: QUALCOMM Incorporated
    Inventors: Shu Du, Ning Zhang, Zhen Xie, James Simon Cho, Chin-Hung Chen, Kai Shi
  • Publication number: 20130202532
    Abstract: Provided herein are high-input detector modules and multi-input biological classifier circuits and systems that integrate sophisticated sensing, information processing, and actuation in living cells and permit new directions in basic biology, biotechnology and medicine. The multi-input biological classifier circuits described herein comprise synthetic, scaleable transcriptional/post-transcriptional regulatory circuits that are designed to interrogate the status of a cell by simultaneously sensing expression levels of multiple endogenous inputs, such as microRNAs. The classifier circuits then compute whether to trigger a desired output or response if the expression levels match a pre-determined profile of interest.
    Type: Application
    Filed: July 22, 2011
    Publication date: August 8, 2013
    Applicants: MASSACHUSETTS INSTITUTE OF TECHNOLOGY, PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Yaakov Benenson, Ron Weiss, Liliana Wroblewska, Zhen Xie
  • Publication number: 20100197006
    Abstract: Disclosed herein are autonomous molecular circuits that can function in cells. The circuits can process logical operations in which one or more input cues are among the operands and produce an appropriate output. Such circuits can be implemented in living cells, e.g., eukaryotic or prokaryotic cells that have been modified to include circuit components. The molecular circuits and cells containing the circuits can be used in a variety of applications including, e.g., diagnostics, therapeutics, and protein production.
    Type: Application
    Filed: October 23, 2009
    Publication date: August 5, 2010
    Applicants: PRESIDENT AND FELLOWS OF HARVARD COLLEGE, TRUSTEES OF PRINCETON UNIVERSITY, Office of Technology Licensing and Intellectual Property
    Inventors: Yaakov Benenson, Ron Weiss, Leonidas Bleris, Zhen Xie