Abstract: Here provided is a method for multiplex nucleic acid analysis. The method includes steps of hybridizing sets of probes to target nucleic acids in a sample, ligating the hybridized probes, amplifying the ligated probes, and assaying the amplification products to determining the presence, absence, or quantity of the target nucleic acids in the sample. The multiplexity is made available in part by adding detectable moieties and inserting stuffer sequences in primers so that amplification products may be identified on the basis of the detectable moieties and fragment sizes. Also provided is a sensitive method of detecting small copy number changes by measuring the copy number of a plurality of target sites in the nucleic acid in a test sample in comparison to a control sample and then determining the copy number of the nucleic acid based on the measured copy number of the plurality of target sites. Further provided is a kit for multiplex nucleic acid analysis and for small copy number change determination.

Abstract: The present disclosure provides a traffic controlling method based on remote applications, comprising the following steps: evaluating a current network bandwidth; detecting a bitmap updating frequency of each area in a current screen, identifying a multimedia area, and judging whether the multimedia area is valid or not to obtain a judging result; and controlling data traffic according to the current network bandwidth and the judging result. By evaluating the current network bandwidth, detecting the updating frequency of each area in the current screen and identifying whether the multimedia area is valid, and dynamically controlling the data traffic according to the current network bandwidth and according to whether the multimedia area is valid, the present disclosure is capable of ensuring the unblocked transmission of the critical business and preventing the display quality of the multimedia area from being affected to improve the system performance and the user experience.

Abstract: Here provided is a method for multiplex nucleic acid analysis. The method includes steps of hybridizing sets of probes to target nucleic acids in a sample, ligating the hybridized probes, amplifying the ligated probes, and assaying the amplification products to determining the presence, absence, or quantity of the target nucleic acids in the sample. The multiplexity is made available in part by adding detectable moieties and inserting stuffer sequences in primers so that amplification products may be identified on the basis of the detectable moieties and fragment sizes. Also provided is a sensitive method of detecting small copy number changes by measuring the copy number of a plurality of target sites in the nucleic acid in a test sample in comparison to a control sample and then determining the copy number of the nucleic acid based on the measured copy number of the plurality of target sites. Further provided is a kit for multiplex nucleic acid analysis and for small copy number change determination.

Abstract: The present disclosure provides a traffic controlling method based on remote applications, comprising the following steps: evaluating a current network bandwidth; detecting a bitmap updating frequency of each area in a current screen, identifying a multimedia area, and judging whether the multimedia area is valid or not to obtain a judging result; and controlling data traffic according to the current network bandwidth and the judging result. By evaluating the current network bandwidth, detecting the updating frequency of each area in the current screen and identifying whether the multimedia area is valid, and dynamically controlling the data traffic according to the current network bandwidth and according to whether the multimedia area is valid, the present disclosure is capable of ensuring the unblocked transmission of the critical business and preventing the display quality of the multimedia area from being affected to improve the system performance and the user experience.

Abstract: A VPN-based method for a mobile communication terminal to access data securely comprises: when a data security device is operating in the mobile communication terminal, the data security device allows the mobile communication terminal to access an intranet but inhibits the mobile communication terminal from accessing an external network; and when the data security device is not operating in the mobile communication terminal, a VPN server inhibits the mobile communication terminal from accessing the intranet. The data security device is disposed in the mobile communication terminal. The data security device cooperates with the VPN server to inhibit the user of the mobile communication terminal from sending protected files to the external network via a network when the data security device is deactivated and to inhibit applications running on the data security device from accessing networks outside the VPN resources to release the protected files to the external network.