Abstract: The present invention relates to the field of medical appliances, and specifically, to a disposable pen-type blood-return-visible and anti-reflux venous blood collection needle, comprising a venous puncture needle and a transparent needle seat. A one-way valve fixation seat is arranged in a cavity of the transparent needle seat, and an anti-reflux one-way valve is mounted on the one-way valve fixation seat. The one-way valve fixation seat is plugged from a front end of the needle seat and mounted in the cavity of the needle seat. A tail end of the venous puncture needle is plugged and mounted in a blood inlet of the one-way valve fixation seat. A tail end of a puncture-end protective cover is sleeved over an outer wall of a front portion of the needle seat. A tube-plug puncture needle is plugged and mounted in a blood outlet on a tail portion of the needle seat, and a latex sheath is sleeved over the tube-plug puncture needle.