Abstract: The present disclosure relates to a two-plasmid system for prime editing in yeast, use thereof, and a method for gene prime editing in yeast. The two-plasmid system includes a first plasmid and a second plasmid. The first plasmid includes a sequence encoding for an epegRNA. The epegRNA is an RNA molecule including a motif at a 3?-terminus of a pegRNA, and the motif having a sequence as set forth in SEQ ID NO. 12. The second plasmid includes a sequence encoding for a fusion protein of a nucleic acid nickase nCas9 fused with a reverse transcriptase M-MLV RT. The two-plasmid expression system can be used in gene editing in yeast.

Abstract: This application provides a polygonal spherical sampling device, belonging to the technical field of spherical field antenna measurement, and including a probe, a mounting rack and a supporting platform. The supporting platform is mounted on the mounting rack for placing an object to be tested. The number of the probes is more than or equal to six, the probes are mounted on the mounting rack and a plurality of the probes are distributed on at least two vertical planes in a three-dimensional spherical space and are arranged around the supporting platform. The vertical planes are arranged symmetrically about a vertical axis, the probes are uniformly distributed at intervals of A degrees on each vertical plane, the probes on a same horizontal plane are uniformly distributed at intervals of B degrees, both A and B are less than or equal to 90.

Abstract: This application provides a polygonal spherical sampling device, belonging to the technical field of spherical field antenna measurement, and including a probe, a mounting rack and a supporting platform. The supporting platform is mounted on the mounting rack for placing an object to be tested. The number of the probes is more than or equal to six, the probes are mounted on the mounting rack and a plurality of the probes are distributed on at least two vertical planes in a three-dimensional spherical space and are arranged around the supporting platform. The vertical planes are arranged symmetrically about a vertical axis, the probes are uniformly distributed at intervals of A degrees on each vertical plane, the probes on a same horizontal plane are uniformly distributed at intervals of B degrees, both A and B are less than or equal to 90.