Abstract: The present invention provides a tumor marker and an application thereof. The tumor marker is located on the chromosome 1, chromosome 2, chromosome 3, chromosome 5, chromosome 7, chromosome 8, chromosome 10, chromosome 11, and chromosome 21 of the human genome, and comprises one or more CpG sites capable of undergoing methylation modification. The present invention provides a class of DNA epigenetic modification-related tumor markers that exhibit a significant high methylation status in tumor patients. The tumor markers can be used for clinical assisted screening, diagnosis, prognosis and the like of tumors, or can be used for designing diagnostic reagents and kits.

Abstract: A method for detecting nucleic acid comprises the following steps: S1, performing isothermal amplification of nucleic acid to be detected; S2, performing secondary amplification of the product of step S1, and the product of step S2 is a nucleic acid single strand which can be alternatively produced by other ways; S3, hybridizing the product of step S2 with a probe; S4, degrading the probe: a nuclease is added into the hybridized product to degrade redundant single-strand nucleic acid; and, S5, detecting the product obtained in step S4. A kit for detecting nucleic acid comprises an isothermal amplification primer, a secondary amplification enzyme, a probe, a nuclease and a test strip. A use thereof comprises detection of pathogenic bacteria, viruses, transgenic nucleic acid, genetic material variation, tumor and DNA methylation variation. The aforementioned technical solution is rapid, specific and sensitive without a requirement of complex instruments and equipment.

Abstract: The present invention provides a target sequence of an RNA virus. The target sequence is a nucleic acid sequence fragment in the gene sequence in the RNA virus containing 20-40 bases and having not less than 95% similarity to genome sequence of human or related species such as livestock and poultry. The above-mentioned target sequence of the RNA virus is selected from SEQ ID NO. 1 - SEQ ID NO. 615. The present invention also relates to a primer composition for constructing the above-mentioned target sequence, biomaterials such as antisense RNA related to the above-mentioned target sequence, and related uses such as design of a vaccine lacking the target sequence. The virus fragment with the above-mentioned sequence constructed in the present invention has the function of interacting with human genomic DNA and is similar to viral miRNA.

Abstract: The present invention discloses a drug for treating severe acute respiratory syndrome caused by coronavirus and the complications thereof and the use of the drug, and specifically the use of an agent in the preparation of a drug for treating severe acute respiratory syndrome caused by coronavirus and the complications thereof. The above-mentioned agent is at least one of hyaluronic acid synthesis inhibitor, hyaluronidase, hyaluronidase inhibitor, hyaluronic acid receptor inhibitor, and anticoagulant.

Abstract: Provided are an isolated polynucleotide and an application thereof. The isolated polynucleotide is a polynucleotide shown in SEQ ID NO:1 or a fragment or complementary nucleic acid thereof with a modified CpG site. Further provided are an application of the polynucleotide for preparation of a tumor detection reagent or kit, a preparation method therefor, a reagent and an application thereof, a kit, and a method for in vitro detection of the methylome of a sample.

Abstract: Provided are an isolated polynucleotide and a use thereof, wherein the isolated polynucleotide is the polynucleotide shown in SEQ ID NO:1 or a fragment or complementary nucleic acid thereof at a modified CpG site. Further provided are the use of the polynucleotide for preparing a tumor detection reagent or a kit, a preparation method, a reagent and a use thereof, a kit, and a method for the in-vitro detection of the methylation profile of a sample.

Abstract: Provided are an isolated polynucleotide and a use thereof, wherein the polynucleotide is a polynucleotide as shown in SEQ ID NO:1, a fragment with a modified CpG site, or a complementary nucleic acid. Also provided are the use of the polynucleotide for preparing a tumor detection reagent or a kit, a preparation method therefor, a reagent and a use thereof, a kit and a method for detecting the methylation profile of samples in vitro.

Abstract: The present invention provides a methylation tumor marker STAMP-EP3 and use thereof, and belongs to the field of disease diagnosis markers. The present invention provides use of the methylation tumor marker STAMP-EP3 in the preparation of a tumor diagnosis reagent.

Abstract: The present invention provides a methylated tumor marker STAMP-EP5 and an application thereof, relating to the technical field of disease diagnosis markers. The present invention further provides an application of methylated tumor marker STAMP-EP5 in the preparation of tumor diagnostic reagents.

Abstract: The present invention relates to the field of disease diagnosis markers, and provides a methylation tumor marker STAMP-EP6, and an application thereof. The present invention provides an application of the methylation tumor marker STAMP-EP6 in preparing a cancer diagnosis reagent.

Abstract: The present invention relates to the field of disease diagnosis markers, and provides a methylation tumor marker STAMP-EP4, and an application thereof. The present invention provides an application of the methylation tumor marker STAMP-EP4 in preparing a cancer diagnosis reagent.

Abstract: A methylated tumor marker STAMP-EP1 and a use thereof, which relate to the field of molecular biology. The present disclosure further relates to a use of the methylated tumor marker STAMP-EP1 in the preparation of tumor diagnostic agents. The tumor marker STAMP-EP1 herein is hypermethylated in all tumor types, is hypomethylated in corresponding normal tissue, and with very high sensitivity and specificity; primers for detecting STAMP-EP1 may be used to prepare a tumor diagnostic kit.

Abstract: Provided is a methylated tumor marker STAMP-EP2 and uses thereof in preparing a tumor diagnostic reagen. The tumor marker STAMP-EP2 herein is hypermethylated in all tumor types, is hypomethylated in corresponding normal tissue, and has high sensitivity and specificity; primers for detecting STAMP-EP2 may be used to prepare a tumor diagnostic kit.