Abstract: A tissue chip core-making system based on image recognition and positioning and a core-making method thereof—includes a cutting system and a computer control system. The cutting system includes a numerical control cutting machine, XYZ-axis translation worktables, a 360° rotation turntable, a recipient wax block rack, a freezing table and an image recognition and positioning module. A core-making process based on the core-making system includes: lofting, sample position recognition, tissue sample image acquisition, tissue sample image processing, cutting parameter setting, tissue sample cutting, tissue core information recognition and storage to obtain a tissue core with information traceability. The method obtains a coring region through visual recognition, and has the characteristics of high automation degree and high work efficiency.