Abstract: The present disclosure relates to methods of enzymatic treatment of double-stranded PCR amplified products for eliminating or minimizing primer-dimers in multiplex PCR reactions and for the efficient ligation of adapters. The present disclosure relates to methods and compositions that allow more efficient highly multiplex target amplification compared to conventional methods, compositions and kits by minimizing laboratory steps, eliminating primer-dimers and increasing the efficiency of adapter ligation. The disclosed methods use multiple target-specific primers for specific and selective amplification of targets in a subject's genome. The disclosed methods can be used for numerous downstream procedures and analysis, including DNA sequencing.

Abstract: The present disclosure relates to a method of target enrichment and amplification of selective target regions of genomes or nucleic acid samples and further analysis by systems such as NGS. The disclosure provides methods and kits that can be used in numerous downstream procedures including DNA sequencing. The disclosed methods can be utilized to sequence the target nucleic acid sequences such as to detect the presence of genetic variations for biological assays, assessment of disease, to count copies of target regions, and to allow such target enrichment prior to sequencing.

Abstract: The present disclosure relates to compositions and methods for detection, identification and quantification of human papillomaviruses (HPV) and clinically significant sexually transmitted infections (STI) in a single amplification reaction. The disclosed method utilizes next-generation sequencing (NGS) to sequence amplified products. The present disclosure is also directed to kits containing primers specific to multiple HPV and STI targets.

Abstract: The present disclosure relates to methods of enzymatic treatment of double-stranded PCR amplified products for eliminating or minimizing primer-dimers in multiplex PCR reactions and for the efficient ligation of adapters. The present disclosure relates to methods and compositions that allow more efficient highly multiplex target amplification compared to conventional methods, compositions and kits by minimizing laboratory steps, eliminating primer-dimers and increasing the efficiency of adapter ligation. The disclosed methods use multiple target-specific primers for specific and selective amplification of targets in a subject's genome. The disclosed methods can be used for numerous downstream procedures and analysis, including DNA sequencing.

Abstract: The present disclosure provides an electromagnetic heating device, comprising: an electromagnetic heating unit, an infrared heating unit and a MCU. The MCU is coupled with the electromagnetic heating unit and the infrared heating unit, so as to control the electromagnetic heating unit and the infrared heating unit to heat individually or simultaneously. The electromagnetic heating device of the present disclosure, since it comprises an electro electromagnetic heating unit and an infrared heating unit, the heating of the heating appliances of different materials can be performed, the application thereof is wide and unrestricted. In addition, since the infrared heating unit is comprised, the maximum heating power thereof is not limited by that of the coil disk.

Abstract: The present disclosure relates to compositions and methods for detection, identification and quantification of human papillomaviruses (HPV) and clinically significant sexually transmitted infections (STI) in a single amplification reaction. The disclosed method utilizes next-generation sequencing (NGS) to sequence amplified products. The present disclosure is also directed to kits containing primers specific to multiple HPV and STI targets.

Abstract: The present disclosure relates to a method of target enrichment and amplification of selective target regions of genomes or nucleic acid samples and further analysis by systems such as NGS. The disclosure provides methods and kits that can be used in numerous downstream procedures including DNA sequencing. The disclosed methods can be utilized to sequence the target nucleic acid sequences such as to detect the presence of genetic variations for biological assays, assessment of disease, to count copies of target regions, and to allow such target enrichment prior to sequencing.

Abstract: Disclosed is an electromagnetic heating control circuit, comprising a control chip, a rectifier filter circuit, a resonant capacitor, a switching transistor, a drive circuit, and a synchronous voltage detection circuit. The switching transistor comprises a first end, a second end, and a control end. The first end is connected to a positive output end of the rectifier filter circuit by using the resonant capacitor. The second end is connected to a negative output end of the rectifier filter circuit by using a current limiting resistor. The control chip comprises a positive phase voltage input end, a negative phase voltage input end, a voltage detection end, and a signal input end. The positive phase voltage input end and the negative phase voltage input end detect voltages at two ends of the resonant capacitor by using the synchronous voltage detection circuit. The signal output end is connected to the control end by using the drive circuit.

Abstract: The present disclosure provides an electromagnetic heating device, comprising: an electromagnetic heating unit, an infrared heating unit and a MCU. The MCU is coupled with the electromagnetic heating unit and the infrared heating unit, so as to control the electromagnetic heating unit and the infrared heating unit to heat individually or simultaneously. The electromagnetic heating device of the present disclosure, since it comprises an electro electromagnetic heating unit and an infrared heating unit, the heating of the heating appliances of different materials can be performed, the application thereof is wide and unrestricted. In addition, since the infrared heating unit is comprised, the maximum heating power thereof is not limited by that of the coil disk.

Abstract: Disclosed is an electromagnetic heating control circuit, comprising a control chip, a rectifier filter circuit, a resonant capacitor, a switching transistor, a drive circuit, and a synchronous voltage detection circuit. The switching transistor comprises a first end, a second end, and a control end. The first end is connected to a positive output end of the rectifier filter circuit by using the resonant capacitor. The second end is connected to a negative output end of the rectifier filter circuit by using a current limiting resistor. The control chip comprises a positive phase voltage input end, a negative phase voltage input end, a voltage detection end, and a signal input end. The positive phase voltage input end and the negative phase voltage input end detect voltages at two ends of the resonant capacitor by using the synchronous voltage detection circuit. The signal output end is connected to the control end by using the drive circuit.