Patents by Inventor Zhihua PEI

Zhihua PEI has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for constructing high-resolution single cell Hi-C library with a lot of information
    Patent number: 10900031
    Abstract: Provided in the present invention is a method for constructing a high-resolution single cell Hi-C library with a large amount of information, comprising the following steps: Step B: obtain a small amount of fixed chromatin; Step C: digest the fixed chromatin in Step B to obtain fragments of the fixed chromatin; Step D: reconnect the fragments of the fixed chromatin in Step C directly to obtain reconnected fragments of the fixed chromatin; Step E: de-fix the reconnected fragments of the fixed chromatin in Step D to release DNA fragments; Step F: amplify the released DNA fragments in Step E to obtain amplified products; and Step H: construct a sequencing DNA library by using the amplified products as the DNA fragments to be sequenced.
    Type: Grant
    Filed: October 19, 2015
    Date of Patent: January 26, 2021
    Assignees: ZHEJIANG ANNOROAD BIO-TECHNOLOGY CO. LTD., ANNOROAD GENE TECHNOLOGY (BEIJING) CO., LTD.
    Inventors: Xiaolin Li, Jiezhong Zhang, Hongmei Zhao, Zhihua Pei, Zhaoling Xuan, Dawei Li, Junbin Liang, Chongjian Chen
  • METHOD FOR CONSTRUCTING HIGH-RESOLUTION SINGLE CELL HI-C LIBRARY WITH A LOT OF INFORMATION
    Publication number: 20180305685
    Abstract: Provided in the present invention is a method for constructing a high-resolution single cell Hi-C library with a large amount of information, comprising the following steps: Step B: obtain a small amount of fixed chromatin; Step C: digest the fixed chromatin in Step B to obtain fragments of the fixed chromatin; Step D: reconnect the fragments of the fixed chromatin in Step C directly to obtain reconnected fragments of the fixed chromatin; Step E: de-fix the reconnected fragments of the fixed chromatin in Step D to release DNA fragments; Step F: amplify the released DNA fragments in Step E to obtain amplified products; and Step H: construct a sequencing DNA library by using the amplified products as the DNA fragments to be sequenced.
    Type: Application
    Filed: October 19, 2015
    Publication date: October 25, 2018
    Applicants: NANJING ANNOROAD GENE TECHNOLOGY CO., LTD, ANNOROAD GENE TECHNOLOGY (BEIJING) CO., LTD
    Inventors: Xiaolin LI, Jiezhong ZHANG, Hongmei ZHAO, Zhihua PEI, Zhaoling XUAN, Dawei LI, Junbin LIANG, Chongjian CHEN