Abstract: A control method for a heating system of an air conditioner, the method includes: obtaining ambient temperature and external heat exchanger evaporation temperature; obtaining external heat exchanger evaporation pressure, and obtaining, according to the external heat exchanger evaporation pressure, corresponding saturation temperature; and controlling a base plate heating device according to the ambient temperature, the external heat exchanger evaporation temperature, or the saturation temperature. The base plate heating device is controlled according to the ambient temperature, the saturation temperature corresponding to the external heat exchanger evaporation pressure, or the external heat exchanger evaporation temperature, and may avoid freezing of the base plate of an air conditioner, and may ensure normal drainage of the base plate during defrosting to improve the stability and reliability of the air conditioner.

Abstract: The electric control box includes a box body, a mounting plate, a heat sink, an electronic component and a heat dissipation fan. The box body is provided with a mounting cavity. The mounting board is arranged in the mounting cavity to form a first chamber and a second chamber located on two sides of the mounting board. A first air vent and a second air vent are arranged on the mounting board and are spaced apart. The first air vent and the second air vent are connected to the first chamber and the second chamber. The heat sink is at least partially arranged in the first chamber. The electronic component is arranged in the second chamber and in thermally conductive connection with the heat sink. The heat dissipation fan is used for blowing air and from the first chamber into the second chamber via the first air vent.

Abstract: Proposed by the present disclosure are an air conditioner, a control method and a computer-readable storage medium. The air conditioner includes: a refrigerant switching device, an indoor heat exchanger, an outdoor heat exchanger, a compressor, a memory, and a processor. The refrigerant switching device includes a liquid pipe, a gas pipe, and a valve assembly. The processor executes a computer program to execute the following: obtaining switching information of a working mode of the air conditioner; and controlling the valve assembly according to the switching information and the gas pipe and the liquid pipe are closed according to the sequence of the gas pipe first and then the liquid pipe, and then opened according to the sequence of the gas pipe first and then the liquid pipe.

Abstract: The invention is a method of preventing the generation of pink color during antibody manufacturing by either preventing the reduction of antibodies during manufacturing and harvest or inhibiting the conversion of cyanocobalamin (CN-Cbl) to hydroxocobalamin (HO-Cbl) during manufacturing and harvest. Replacement of white light in the cell culture media preparation and storage areas with red light inhibits the conversion of CN-Cbl to HO-Cbl. Addition of peroxides to the clarified bulk at harvest inhibits reduction of the antibody disulfide bonds.

Abstract: The present invention discloses a composite device for high-precision laser additive/subtractive manufacturing, which consists of a sealed shaping chamber, an inert protective gas source and a machine-shaping platform; wherein, the inert protective gas source is connected to the sealed shaping chamber; and the machine-shaping platform is arranged in the sealed shaping chamber, as well as there is a light path selection system is arranged right above the machine-shaping platform; in addition, there is a machining position is equipped on the machine-shaping platform, meanwhile, there are lead screws are arranged under the machine-shaping platform.

Abstract: In certain embodiments, the disclosure provides an IgG4 antibody comprising a heavy chain and a light chain, wherein the heavy chain comprises: (a) a modified IgG4 CH1 region having a substitution of the lysine residue at position 196; or (b) a modified IgG4 hinge region having a substitution of the serine residue at position 217, the glycine residue at position 220, the proline residue at position 224 or the proline residue at position 225. Preferably, the IgG4 antibody further comprises a substitution of the serine residue at position 228 in the heavy chain hinge region.

Abstract: A two-pipe enhanced-vapor-injection outdoor unit and a two-pipe enhanced-vapor-injection multi-split system are provided. The two-pipe enhanced-vapor-injection outdoor unit includes an outdoor heat exchanger, an enhanced-vapor-injection compressor, a reversing assembly, a super cooler, a throttling assembly and a first pipe. The reversing assembly includes a first end and a second end connected with a gas discharge port and a gas return port, respectively. A main heat-exchange flow path is connected with the first port and the second port, respectively. An auxiliary heat-exchange flow path is connected with the injection port. The throttling assembly includes two ends connected with an outlet of the main heat-exchange flow path and an inlet of the outdoor heat exchanger. The first pipe includes a first end connected with an outlet of the outdoor heat exchanger, and a second end arranged between the throttling assembly and the main heat-exchange flow path.

Abstract: In certain embodiments, the invention provides a method of purifying a protein of interest from a mixture which comprises the protein of interest and one or more contaminants, comprising: (a) contacting the mixture with a dextran polymer under conditions suitable for the dextran polymer to bind to one or more contaminants, thereby to form a contaminant precipitate; (b) separating the contaminant precipitate from the mixture to form a solution, thereby purifying the protein of interest.

Abstract: The present disclosure discloses an air conditioner controlling method and apparatus, as well as an air conditioner having the same. The method includes: determining that an outdoor heat exchanger of the air conditioner runs in an evaporator state; acquiring a state signal of a refrigerant of an indoor refrigerating unit; and adjusting an opening degree of an electronic expansion valve of an outdoor unit according to the state signal of the indoor refrigerating unit. This method may adjust the opening degree of the electronic expansion valve of the outdoor unit according to the state signal of the indoor refrigerating unit when the outdoor heat exchanger of the air conditioner runs in the evaporator state, thereby effectively improving the reliability of control, broadening the reliable operation range of the system, and reasonably distributing the refrigerant between the indoor refrigerating unit and the outdoor unit under a low temperature working condition.

Abstract: The present invention discloses a composite device for high-precision laser additive/subtractive manufacturing, which consists of a sealed shaping chamber, an inert protective gas source and a machine-shaping platform; wherein, the inert protective gas source is connected to the sealed shaping chamber; and the machine-shaping platform is arranged in the sealed shaping chamber, as well as there is a light path selection system is arranged right above the machine-shaping platform; in addition, there is a machining position is equipped on the machine-shaping platform, meanwhile, there are lead screws are arranged under the machine-shaping platform.

Abstract: A heat pump system (100) and an air conditioner are provided. The heat pump system (100) includes a compressor assembly (10), an outdoor heat exchanger (20), an indoor heat exchanger (30), a heating and heat accumulation device (50) and a switching device (40). The heating and heat accumulation device (50) is connected in series with the switching device (40). In the first heating mode, a refrigerant discharged out of the compressor assembly (10) enters the indoor heat exchanger (30) and the outdoor heat exchanger (20) in sequence after passing through the switching device (40) and the heating and heat accumulation device (50). In the defrosting mode, the refrigerant discharged out of the compressor assembly (10) enters the indoor heat exchanger (30), the outdoor heat exchanger (20) and the heating and heat accumulation device (50) in sequence after passing through the switching device (40).

Abstract: A method and a device for controlling a low-temperature refrigeration air valve, the method includes: acquiring a current environment temperature; according to the current environment temperature, determining an initial target pressure of the current system and an initial opening degree of an air valve; acquiring an actual pressure of the current system and a target pressure of the current system; and adjusting an opening degree of the air valve according to a difference value between the target pressure of the current system and the actual pressure of the current system, wherein the air valve is disposed at a low-temperature cover. An opening degree of the air valve is adjusted according to the difference value between the target pressure of the current system and the actual pressure of the current system, to meet the refrigeration demand of the user in an ultra-low temperature environment, expanding the operation scope of refrigeration.

Abstract: A control method and device for a low-temperature cooling air valve include: obtaining an actual system pressure, an ambient temperature, and a system energy requirement; and determining an opening degree pre according to the actual system pressure, the ambient temperature, and the system energy requirement. The control method is employed to perform combined control on the low-temperature cooling air valve according to the system high pressure, the ambient temperature and the system energy requirement, and can refine the range of opening degrees of the low-temperature cooling air valve and determine an opening degree thereof, thereby facilitating cooling operation of a multi-split air conditioning system in an ultra-low temperature environment, while also increasing the reliability of the cooling operation at low temperature and expanding the operation range.

Abstract: An air conditioner, and a control method and device for a heating system thereof, wherein the method comprises: obtaining ambient temperature and external heat exchanger evaporation temperature; obtaining external heat exchanger evaporation pressure, and obtaining, according to the external heat exchanger evaporation pressure, corresponding saturation temperature; and controlling a base plate heating device according to the ambient temperature, the external heat exchanger evaporation temperature, or the saturation temperature. Hence, the base plate heating device is controlled according to the ambient temperature, the saturation temperature corresponding to the external heat exchanger evaporation pressure, or the external heat exchanger evaporation temperature, to avoid freezing of the base plate of an air conditioner, and ensure normal drainage of the base plate during defrosting, thereby improving the stability and reliability of the air conditioner.

Abstract: Methods for the production of high purity recombinant protein such as monoclonal antibodies (mAb) using disulfide bond re-oxidation are provided. In particular, the present disclosure provides methods for converting partial molecules (e.g., antibody fragments) to full molecules (e.g., full antibodies) comprising admixing a starting solution comprising the partial molecules with a redox buffer comprising a redox pair which comprises at least one thiol reducing agent (e.g., cysteine) and at least one thiol oxidizing agent (e.g., cystine), wherein the redox buffer re-oxidizes the partial molecules to full molecules. The disclosed methods can be used, e.g., to prevent or mitigate the formation of partial molecules during protein purification, or to reprocess or rescue a solution comprising partial molecules (e.g., a partially degraded pharmaceutical formulation).

Abstract: A two-pipe enhanced-vapor-injection outdoor unit and a two-pipe enhanced-vapor-injection multi-split system are provided. The two-pipe enhanced-vapor-injection outdoor unit includes an outdoor heat exchanger (10), an enhanced-vapor-injection compressor (16), a reversing assembly (18), a super cooler (20), a throttling assembly (22) and a first pipe (24). The reversing assembly (18) includes a first end and a second end connected with a gas discharge port (162) and a gas return port (164), respectively. A main heat-exchange flow path is connected with the first port (12) and the second port (14), respectively. An auxiliary heat-exchange flow path is connected with the injection port (166). The throttling assembly (22) includes two ends connected with an outlet of the main heat-exchange flow path and an inlet of the outdoor heat exchanger (10).

Abstract: In certain embodiments, the present invention provides a method of purifying a monomeric monoclonal antibody from a mixture which comprises the monomeric monoclonal antibody and one or more contaminants, comprising: a) subjecting the mixture to cation exchange chromatography (CEX) matrix, wherein the monomeric monoclonal antibody binds to the CEX matrix; b) contacting the CEX matrix with a wash solution at a pH which is between about 7 and about 7.8; c) eluting the monomeric monoclonal antibody from the CEX matrix into an elution solution, thereby purifying the monomeric monoclonal antibody.

Abstract: The present disclosure discloses an air conditioner controlling method and apparatus, as well as an air conditioner having the same. The method includes: determining that an outdoor heat exchanger of the air conditioner runs in an evaporator state; acquiring a state signal of a refrigerant of an indoor refrigerating unit; and adjusting an opening degree of an electronic expansion valve of an outdoor unit according to the state signal of the indoor refrigerating unit. This method may adjust the opening degree of the electronic expansion valve of the outdoor unit according to the state signal of the indoor refrigerating unit when the outdoor heat exchanger of the air conditioner runs in the evaporator state, thereby effectively improving the reliability of control, broadening the reliable operation range of the system, and reasonably distributing the refrigerant between the indoor refrigerating unit and the outdoor unit under a low temperature working condition.

Abstract: In certain embodiments, the disclosure provides an IgG4 antibody comprising a heavy chain and a light chain, wherein the heavy chain comprises: (a) a modified IgG4 CH1 region having a substitution of the lysine residue at position 196; or (b) a modified IgG4 hinge region having a substitution of the serine residue at position 217, the glycine residue at position 220, the proline residue at position 224 or the proline residue at position 225. Preferably, the IgG4 antibody further comprises a substitution of the serine residue at position 228 in the heavy chain hinge region.

Abstract: In certain embodiments, the invention provides a method of purifying a protein of interest from a mixture which comprises the protein of interest and one or more contaminants, comprising: (a) contacting the mixture with a dextran polymer under conditions suitable for the dextran polymer to bind to one or more contaminants, thereby to form a contaminant precipitate; (b) separating the contaminant precipitate from the mixture to form a solution, thereby purifying the protein of interest.