Abstract: One or more embodiments of the present description provide a method and device for risk prediction of thermal runaway in LIB. The method includes: acquiring knowledge of a mechanism for thermal runaway in LIB; describing an evolution process of thermal runaway in LIB by adopting a fault tree; mapping a fault tree structure to a dynamic Bayesian network model for thermal runaway in LIB to obtain quantitative results of a risk of thermal runaway in LIB; and taking the quantitative results of a dynamic Bayesian network as inputs of a machine learning model to obtain prediction results of the risk of thermal runaway. By using the method in the present embodiment, an evolution trend of battery thermal runaway can be predicted by fusing multiple thermal runaway causes and multi-source data, and thus, the prediction results are relatively accurate.

Abstract: The invention provides a glutamate decarboxylase mutant with improved pH tolerance and use thereof in synthesis of gamma-aminobutyric acid. The mutant is obtained by mutating glutamate decarboxylase having an amino acid sequence as shown in SEQ ID NO. 3. The enzyme activity of the mutant at pH 6.5 is improved to 178% of the original enzyme (SEQ ID NO. 3). The final yield of 1000 g of substrate fed in batches in a 5L tank for 12 h is up to 688.13 g/L, which is about 52% higher than the productivity of the original glutamate decarboxylase. The final molar conversion rate can reach 98.2%. The invention not only broadens the enzyme activity of GAD under the optimum pH, but also broadens the enzyme activity of GAD under the neutral pH, and enhances the capability of the GAD to synthesize gamma-aminobutyric acid, and therefore is more suitable for industrial production.

Abstract: A female connector for receiving a male connector includes a housing, a support ring, a clip, and a clip blocking member. The male connector includes a groove. The housing includes an outer surface and housing openings. The support ring includes support ring openings. The clip includes an operation portion between a pair of clip legs. The pair of clip legs can pass through the housing and support ring openings. The clip blocking member is located outside the housing and has a pair of blocking faces which face the pair of clip legs. When the groove aligns with the housing and support ring openings, the pair of clip legs enter the groove, and the operation portion moves to a travel end position. In the travel position, the distal ends of the pair of clip legs are between the pair of blocking faces and the outer surface.

Abstract: The present disclosure provides a female connector for receiving a male connector. The female connector includes a housing, a support ring, a clip having legs, and a clip blocking member having blocking faces. The male connector includes a groove and can be at least partially inserted into an internal space of the support ring located in a fluid channel of the female connector. When the groove is aligned with housing openings and support ring openings, the clip legs can enter the groove, and an operation portion of the clip can get into a travel end position. When the groove is not aligned with the housing openings and the support ring openings, and the clip legs abut against an outer surface of the male connector, the blocking faces can prevent the operation portion from getting into the travel end position by abutting against distal ends of the clip legs.

Abstract: The observed over-expression of Sp1 target genes has inspired inventors to formulate a specific strategy for correcting many gene expression defects, and thus improve clone development. The invention is based on the belief that manipulating Sp1 activity can improve cloning. Inventors believe that cloning is inefficient in large part because of the continued expression of Sp1 target genes in the early cloned embryos, which causes clones to be very unlike normal embryos, and so makes them very unhealthy. Inventors propose that if over-expression of Sp1 target genes is prevented in early stage clones, this would greatly improve cloning efficiency by making the cloned embryos healthy again.