Abstract: A packaging system for a coronavirus pseudovirus, including a vesicular stomatitis virus (VSV) vector in which Fluc and EGFP dual-reporter genes replace a GP gene, and packaging cell that expresses a coronavirus spike protein. The packaging system may quickly package pseudoviruses by using a one-step packaging method, and may be used in the research of coronaviruses such as COVID-19 (SARS-CoV-2), SARS (SARS-CoV) and MERS, and other viruses. The packaging system and thereby pseudovirus method may also be used to evaluate the efficacy of disinfectants by means of virus contamination distribution models, setting up scenarios, and sampling and testing steps.

Abstract: An observation system and determination method for the re-suspension quantity of submarine sediments by deep-sea internal waves. The observation system comprises a submarine observation platform, a mooring rope, and an anchor mooring counterweight. Acoustic release transponder, a single-point current meter, a turbidity meter, a high-precision temperature and salinity detector, and a sediment catcher are mounted on the submarine observation platform. A main floating body and auxiliary floating bodies are arranged on the mooring rope, wherein the main floating body is located in the middle of the mooring rope and is equipped with acoustic Doppler current profilers, and the auxiliary floating bodies are equipped with turbidity meters and high-precision temperature and salinity detectors. The anchor mooring counterweight is a gravity anchor provided with a square clamping groove and a fixed ring.

Abstract: An observation system and determination method for the re-suspension quantity of submarine sediments by deep-sea internal waves. The observation system comprises a submarine observation platform, a mooring rope, and an anchor mooring counterweight. Acoustic release transponder, a single-point current meter, a turbidity meter, a high-precision temperature and salinity detector, and a sediment catcher are mounted on the submarine observation platform. A main floating body and auxiliary floating bodies are arranged on the mooring rope, wherein the main floating body is located in the middle of the mooring rope and is equipped with acoustic Doppler current profilers, and the auxiliary floating bodies are equipped with turbidity meters and high-precision temperature and salinity detectors. The anchor mooring counterweight is a gravity anchor provided with a square clamping groove and a fixed ring.

Abstract: A centrifugal coolant pump, comprising a water inlet coupling flange(1), an impeller (2), a volute casing (3), a bearing (4), a bearing housing (5), a transmission shaft (6), and fasteners (7). The bearing (4) is interference installed in the bearing housing (5). The transmission shaft (6) and the bearing (4) inner race are interference fitted together. The impeller (2) is interference installed on the transmission shaft (6). The water inlet coupling flange (1) and the bearing housing (5) are separately disposed on the two sides of the volute casing (3) and fixedly connected via the fasteners (7). The water inlet coupling (1) is provided with a first water inlet (A11), and the volute casing (3) is provided with a second water inlet (B12). The impeller (2) mates with the volute casing (3) to form an independent high-temperature flow path (9) and a low-temperature flow path (8).