Abstract: Methods and compositions for making DNA libraries for massive parallel next generation sequencing (NGS), comprises two parts. These methods may be referred to as Triseq sequencing. The first part includes ligating a UMI adapter, amplifying the DNA fragments in the presence of dUTP, enriching the target molecules through primer extension by using a panel of both forward and reverse primers, and removing the dU-containing template DNA. The DNA molecules are organized to primary clones and subclones, labeled by the UMI on 5? and 3? end of the DNA fragments, respectively. The second part includes sequencing the DNA library by NGS, deducing consensus sequence from each subclone, and from within each primary clone, and between the consensus sequences obtained from both forward and reverse primers.

Abstract: Methods, apparatuses and compositions for generating highly sensitive and accurate sequencing results of massive parallel sequencing (NGS). The methods and compositions may be referred to as SubDivision-Seq, and may comprise two parts. The first part includes making a target-enriched DNA library, organizing the UMIs on DNA molecules to form primary clones and subdividing the primary clones into subclones. The second part includes sequencing the DNA library by NGS, deducing consensus sequence from each subclone, then deducing consensus sequence in each primary clone.

Abstract: Disclosed are an expansion tool assembly, comprising: an expandable tubular; a guide shoe partially inserted into and connected to the lower end of the expandable tubular; a sealing sleeve arranged in the expandable tubular and connected to the upper end of the guide shoe; an expansion assembly comprising an expansion cone and expansion seat disposed inside the expandable tubular, wherein the lower end of the expansion seat is connected with hooks, which can be rested on the upper end of the sealing sleeve; an upper joint that is connected to the upper end of the expansion seat and can be connected to a drill string. A force transfer path can be established via connection between the expansion seat and sealing sleeve, so that expandable tubular weight is borne on the drill string. Unintentional expansion of the expandable tubular, caused by interaction between expandable tubular and expansion cone, can be avoided.

Abstract: Disclosed are an expansion tool assembly, comprising: an expandable tubular; a guide shoe partially inserted into and connected to the lower end of the expandable tubular; a sealing sleeve arranged in the expandable tubular and connected to the upper end of the guide shoe; an expansion assembly comprising an expansion cone and expansion seat disposed inside the expandable tubular, wherein the lower end of the expansion seat is connected with hooks, which can be rested on the upper end of the sealing sleeve; an upper joint that is connected to the upper end of the expansion seat and can be connected to a drill string. A force transfer path can be established via connection between the expansion seat and sealing sleeve, so that expandable tubular weight is borne on the drill string. Unintentional expansion of the expandable tubular, caused by interaction between expandable tubular and expansion cone, can be avoided.

Abstract: High throughput methods and compositions (e.g., kits) for the amplification of RNA fragments, including in particular, for the detection of fusion mutations in a high volume of samples, e.g., by high throughput sequencing method. These methods may include barcoding cDNA preparations with template switching reactions, indexing pools of libraries and intensive use of automatic liquid handling, and providing a ready-to-sequence library mix.

Abstract: Methods, compositions and systems to amplify targets from RNA samples. The methods comprise designing target-specific primers, converting RNA into cDNA by reverse transcription, adding a universal primer by using template switching, and amplifying the targets by using multiplex PCR. The methods, compositions and systems described herein may further include, or include the use of, next generation sequencing (NGS) to analyze the sequences and various mutations of the amplified targets.

Abstract: Laboratory-based high throughput methods and compositions (e.g., kits) for the detection of pathogen mRNA, including in particular, for the detection of SARS-CoV-2 RNA from up to 85,000 samples in one Illumina NovaSeq sequencing run. These methods may include barcoding cDNA, indexing pools of libraries and intensive use of automatic liquid handling, providing a ready-to-sequence library mix. These methods and compositions may allow a very inexpensive per sample cost and an assay that may be performed in about one day or less.

Abstract: The present invention discloses an expandable liner hanger system comprising a central pipe and an expandable pipe, with an expansion cone provided therebetween the central pipe and expandable pipe in a way that the expansion cone is movable along the central pipe, so that the expandable pipe is expanded; wherein, the expansion cone comprises an expansion cone seat move along the central pipe, and an expansion cone sleeve fitted over the expansion cone seat, the connection between the expansion cone seat and the expansion cone sleeve is configured in a way that the expansion cone sleeve can move upward together with the expansion cone seat along the central pipe so that the expandable pipe is expanded, and the central pipe can drive the expansion cone seat to disengage from the expansion cone sleeve.

Abstract: The present invention discloses an expandable liner hanger system comprising a central pipe and an expandable pipe, with an expansion cone provided therebetween the central pipe and expandable pipe in a way that the expansion cone is movable along the central pipe, so that the expandable pipe is expanded; wherein, the expansion cone comprises an expansion cone seat move along the central pipe, and an expansion cone sleeve fitted over the expansion cone seat, the connection between the expansion cone seat and the expansion cone sleeve is configured in a way that the expansion cone sleeve can move upward together with the expansion cone seat along the central pipe so that the expandable pipe is expanded, and the central pipe can drive the expansion cone seat to disengage from the expansion cone sleeve.

Abstract: Methods, compositions and systems to amplify short DNA fragments, such as cfDNA, and to reduce random base errors formed in template-dependent primer extension reactions, and to find variant frequencies of mutations on the DNA fragments. The methods, compositions and systems described herein may include, or include the use of, poly(dA) tailing of short DNA fragments by a terminal deoxynucleotidyl transferase, linearly amplification and a multiplex primer extension reaction.

Abstract: A mechanical and hydraulic dual-effect expansion device for well drilling with expandable tubular technology includes an anchor having a lower end connected to an upper end of an insertion connection tube via a coupling, a hydraulic cylinder assembly comprising a hydraulic cylinder and a piston tube movably disposed within an inner cavity of the hydraulic cylinder, and a variable-diameter expansion cone assembly comprising a sealing cone portion and a variable-diameter expansion cone portion, wherein the sealing cone portion has an upper end connected to a lower end of the piston tube and a lower end connected to an upper end of the variable-diameter expansion cone portion. The mechanical and hydraulic dual-effect expansion device can facilitate completion of construction operations of an expandable tubular in a casing and an openhole system.

Abstract: Methods, compositions, systems and kits to amplify or improve amplification of target-specific amplification products by reducing non-specific amplification products (e.g., primer-dimers) when amplifying multiple different nucleotide regions. The methods, compositions, systems and kits described herein may include, or include the use of, one or more resolvases that recognize and bind to and/or cut an aberrant DNA structure.

Abstract: Methods, compositions, systems and kits to introduce a controlled-number of molecular barcodes onto DNA fragments by reducing redundant molecular barcodes formed in template-dependent primer extension reactions, and to find variant frequencies from both strands of DNA. The methods, compositions, systems and kits described herein may include, or include the use of, one or more single-stranded DNA specific nucleases that cleave the single-stranded regions containing unmatched base pairs in amplification products.

Abstract: A mechanical and hydraulic dual-effect expansion device for well drilling with expandable tubular technology includes an anchor having a lower end connected to an upper end of an insertion connection tube via a coupling, a hydraulic cylinder assembly comprising a hydraulic cylinder and a piston tube movably disposed within an inner cavity of the hydraulic cylinder, and a variable-diameter expansion cone assembly comprising a sealing cone portion and a variable-diameter expansion cone portion, wherein the sealing cone portion has an upper end connected to a lower end of the piston tube and a lower end connected to an upper end of the variable-diameter expansion cone portion. The mechanical and hydraulic dual-effect expansion device can facilitate completion of construction operations of an expandable tubular in a casing and an openhole system.

Abstract: Methods, compositions, systems and kits to introduce a controlled-number of molecular barcodes onto DNA fragments by reducing redundant molecular barcodes formed in template-dependent primer extension reactions, and to find variant frequencies from both strands of DNA. The methods, compositions, systems and kits described herein may include, or include the use of, one or more single-stranded DNA specific nucleases that cleave the single-stranded regions containing unmatched base pairs in amplification products.

Abstract: Methods, compositions, systems and kits to amplify or improve amplification of target-specific amplification products by reducing non-specific amplification products (e.g., primer-dimers) when amplifying multiple different nucleotide regions. The methods, compositions, systems and kits described herein may include, or include the use of, one or more resolvases that recognize and bind to and/or cut an aberrant DNA structure.

Abstract: Methods, compositions, systems and kits to amplify or improve amplification of target-specific amplification products by reducing non-specific amplification products (e.g., primer-dimers) when amplifying multiple different nucleotide regions. The methods, compositions, systems and kits described herein may include, or include the use of, one or more resolvases that recognize and bind to and/or cut an aberrant DNA structure.

Abstract: Methods, compositions, systems and kits to amplify or improve amplification of target-specific amplification products by reducing non-specific amplification products (e.g., primer-dimers) when amplifying multiple different nucleotide regions. The methods, compositions, systems and kits described herein may include, or include the use of, one or more resolvases that recognize and bind to and/or cut an aberrant DNA structure.