Abstract: The present disclosure provides an amino acid composition and functional food affecting tumor growth and the use thereof, which falls within the technical field of amino acids. The present disclosure is capable of effectively inhibiting the growth of cancer cells and tumor tissues by strictly limiting the content of serine and glycine and increasing alanine content through cancer cell culture and studies of the transcriptome and metabolic mechanism. Therefore, the functional protein or food developed by the present disclosure, with strictly limited serine and glycine content and increased alanine content, can be advantageous in exerting an anti-tumor effect.