Abstract: Disclosed is a fluorescent PCR method for detecting HLA-B*15:02 allele and a specific primer probe combination. In the present disclosure, a set of primers and probes are designed based on an HLA-B*15:02 specific SNP gene locus by using TaqMan probe technology, combining another set of primers and probes corresponding to the internal reference gene ?-Actin, and a set of primer probe for non-HLA-B*15:02 genes are designed to detect whether a DNA sample contains an HLA-B*15:02 gene and whether a sample is homozygous or heterozygous. Compared with the similar detection methods in the past, the technical scheme in the present disclosure inherits the advantages of high specificity, high throughput, high resolution, low cost, simple and convenient operation, process controllability and the like of the fluorescent PCR, and may detect whether a sample is homozygous or heterozygous.

Abstract: The present disclosure relates to the technical field of biotechnology renewable energy, and more specifically, to a method for producing citric acid by degrading roughage with natural symbiotic mixed culture. The mixed culture YakQH5 is composed of anaerobic fungi (Neocallimastix frontalis) and methanogens (Methanobrevibacter gottschalkii). It was collected in the China General Microbiological Culture Collection Center on Mar. 9, 2020, with the collection number of CGMCC No. 19299. The mixed culture YakQH5 can degrade 15 kinds of roughage respectively and produce a large amount of citric acid. Especially when alfalfa is used as substrate, the yield of citric acid is as high as 46.0 mm. Adding compound antibiotics in the fermentation process can also prevent the mixed culture from being polluted by bacteria in the fermentation process and further improve the efficiency of anaerobic fermentation. The mixed culture YakQH5 has important industrial application value.

Abstract: The present disclosure discloses a pet retrieve device, including a housing subassembly which is a hollow cavity; a circuit board and a battery fixedly arranged in the housing subassembly; a positioning chip arranged on the circuit board; a button and a charging interface which are arranged on the circuit board and penetrates through the housing subassembly; a lamp strip arranged in the housing subassembly, light emitted by the lamp strip being transmitted through the housing subassembly; and a two-dimensional code arranged on a surface of the housing subassembly. A one-to-one correspondence webpage can be opened by scanning the two-dimensional code, and the webpage displays preset pet information. The present disclosure discloses a pet retrieve method. The present disclosure can increase the possibility of retrieving a pet to a certain extent.

Abstract: Performing a file backup includes receiving a file to backup from a source machine and performing a write operation to write the file to a mount point in a file system on a backup server. The backup also includes intercepting a block-level data block to be written which is generated by the write operation; and writing the block-level data block to a corresponding, respective block of a disk image file having a plurality of blocks.

Abstract: Performing a file backup includes receiving a file to backup from a source machine and performing a write operation to write the file to a mount point in a file system on a backup server. The backup also includes intercepting a block-level data block to be written which is generated by the write operation; and writing the block-level data block to a corresponding, respective block of a disk image file having a plurality of blocks.

Abstract: Performing a snapshot backup includes initiating, by a snapshot driver, a snapshot of at least a portion of a filesystem; and receiving, by the snapshot driver, a filesystem bitmap from a backup agent. Subsequently, the snapshot driver performs copy-on-write operations based on the filesystem bitmap.

Abstract: A computer-based system includes a backup server, a production server, a recovery agent that executes on the production server, a database on the production server, a backup database on the backup server, and a meta data file on the backup server. The meta data file includes file names, a file directory, and file attributes. The system is configured to copy the metadata file from the backup server to a production server. When the copying of the metadata file from the backup server to the production server is complete, and before or during a time period in which backup data is copied from the backup server to the production server, the system is configured to receive a request from the production server to restore a file from the backup server with a higher priority than other files on the backup server.

Abstract: A method for providing recovery from a remote server to a client includes sending at least one data restore request to the remote server. A data block identifier are received at the client system via a network. A data block associated with the data block identifier is retrieved from a local backup server associated with the client system if the local back up server stores the data block. The data block associated with the data block identifier is received from the remote server if the local backup server does not store the data block. The data block is restored to the client system.

Abstract: A system includes a production computer machine that includes an operating system and a driver stack. The driver stack includes a file system layer, a recovery driver, a storage layer, a driver layer, a bus driver layer, and a storage device. The system also includes a backup computer processor coupled to the production computer machine via the recovery driver. The recovery driver is configured to commence a recovery of data from the backup computer processor, receive a disk access request from the file system layer, determine if the disk access request accesses data that has not yet been recovered from the backup computer processor, and initiate an on-demand recovery request from the backup computer processor when the data has not been recovered from the backup computer processor.

Abstract: A system includes a proxy processor configured to simulate a virtual disk, and a daemon processor in communication with the proxy processor and a backup repository. The proxy processor is configured to receive from an application a read request or a write request and to direct the read request or the write request to the daemon processor. The daemon processor is configured to receive the read request from the proxy processor and to access the backup repository or the simulated virtual disk, and to receive the write request from the proxy processor and to write data in the write request to the simulated virtual disk.

Abstract: A system includes a production computer machine that includes an operating system and a driver stack. The driver stack includes a file system layer, a recovery driver, a storage layer, a driver layer, a bus driver layer, and a storage device. The system also includes a backup computer processor coupled to the production computer machine via the recovery driver. The recovery driver is configured to commence a recovery of data from the backup computer processor, receive a disk access request from the file system layer, determine if the disk access request accesses data that has not yet been recovered from the backup computer processor, and initiate an on-demand recovery request from the backup computer processor when the data has not been recovered from the backup computer processor.

Abstract: A system includes a production computer machine that includes an operating system and a driver stack. The driver stack includes a file system layer, a recovery driver, a storage layer, a driver layer, a bus driver layer, and a storage device. The system also includes a backup computer processor coupled to the production computer machine via the recovery driver. The recovery driver is configured to commence a recovery of data from the backup computer processor, receive a disk access request from the file system layer, determine if the disk access request accesses data that has not yet been recovered from the backup computer processor, and initiate an on-demand recovery request from the backup computer processor when the data has not been recovered from the backup computer processor.

Abstract: A system and method for testing a computer program using a computer system includes a plurality of computer systems communicating using a network. An interface parser module defines at least one program interface in a program file of a specified program. A fuzzer module reads the program file and identifies the program interfaces. An attack data generator module attacks the program interfaces and communicates with the fuzzer, and the fuzzer determines vulnerabilities in the specified program. A recorder records the attacking procedure. A verifier verifies remedies for vulnerabilities by replaying the attacking procedure of the program interface and determining vulnerabilities. A service status detective module restarts the specified program when the specified program ceases to operate or crashes.

Abstract: A method for providing recovery from a remote server to a client includes sending at least one data restore request to the remote server. A data block identifier are received at the client system via a network. A data block associated with the data block identifier is retrieved from a local backup server associated with the client system if the local back up server stores the data block. The data block associated with the data block identifier is received from the remote server if the local backup server does not store the data block. The data block is restored to the client system.

Abstract: A computer-based system includes a backup server, a production server, a recovery agent that executes on the production server, a database on the production server, a backup database on the backup server, and a meta data file on the backup server. The meta data file includes file names, a file directory, and file attributes. The system is configured to copy the metadata file from the backup server to a production server. When the copying of the metadata file from the backup server to the production server is complete, and before or during a time period in which backup data is copied from the backup server to the production server, the system is configured to receive a request from the production server to restore a file from the backup server with a higher priority than other files on the backup server.

Abstract: A system includes a proxy processor configured to simulate a virtual disk, and a daemon processor in communication with the proxy processor and a backup repository. The proxy processor is configured to receive from an application a read request or a write request and to direct the read request or the write request to the daemon processor. The daemon processor is configured to receive the read request from the proxy processor and to access the backup repository or the simulated virtual disk, and to receive the write request from the proxy processor and to write data in the write request to the simulated virtual disk.

Abstract: A computer-based system includes a backup server, a production server, a recovery agent that executes on the production server, a database on the production server, a backup database on the backup server, and a meta data file on the backup server. The meta data file includes file names, a file directory, and file attributes. The system is configured to copy the metadata file from the backup server to a production server. When the copying of the metadata file from the backup server to the production server is complete, and before or during a time period in which backup data is copied from the backup server to the production server, the system is configured to receive a request from the production server to restore a file from the backup server with a higher priority than other files on the backup server.

Abstract: A system includes a production computer machine that includes an operating system and a driver stack. The driver stack includes a file system layer, a recovery driver, a storage layer, a driver layer, a bus driver layer, and a storage device. The system also includes a backup computer processor coupled to the production computer machine via the recovery driver. The recovery driver is configured to commence a recovery of data from the backup computer processor, receive a disk access request from the file system layer, determine if the disk access request accesses data that has not yet been recovered from the backup computer processor, and initiate an on-demand recovery request from the backup computer processor when the data has not been recovered from the backup computer processor.

Abstract: A computer-based system includes a backup server, a production server, a recovery agent that executes on the production server, a database on the production server, a backup database on the backup server, and a meta data file on the backup server. The meta data file includes file names, a file directory, and file attributes. The system is configured to copy the metadata file from the backup server to a production server. When the copying of the metadata file from the backup server to the production server is complete, and before or during a time period in which backup data is copied from the backup server to the production server, the system is configured to receive a request from the production server to restore a file from the backup server with a higher priority than other files on the backup server.

Abstract: A system and method for testing a computer program using a computer system includes a plurality of computer systems communicating using a network. An interface parser module defines at least one program interface in a program file of a specified program. A fuzzer module reads the program file and identifies the program interfaces. An attack data generator module attacks the program interfaces and communicates with the fuzzer, and the fuzzer determines vulnerabilities in the specified program. A recorder records the attacking procedure. A verifier verifies remedies for vulnerabilities by replaying the attacking procedure of the program interface and determining vulnerabilities. A service status detective module restarts the specified program when the specified program ceases to operate or crashes.