Abstract: Methods of infecting a mosquito with Wolbachia and controlling a mosquito population with the Wolbachia-infected mosquito are provided herein. The method includes microinjecting a suspension including Wolbachia cells and buffer into a target mosquito embryo to produce a Wolbachia-infected G0 female. The suspension including Wolbachia cells and buffer is obtained by homogenizing a donor insect embryo, tissue, and/or whole body infected with Wolbachia to form a homogenate and the Wolbachia cells are separated from the homogenate.

Abstract: The present disclosure discloses a separator of male and female mosquito pupae, comprising a fixed plate (2) configured on an inclined baseplate (1), an adjusting plate (3) spaced from the fixed plate (2), and a guide plate (8), wherein an interval between the adjusting plate (3) and the fixed plate (2) tapers from top to bottom, to a interval exit, and interval adjustments are configured go through the adjusting plate (3) from upper part and lower part of the fixed plate (2). The guide plate (8) is configured below the interval exit. The structure of the present disclosure is simple and reasonable, the mosquito larva, male pupae and female pupae can be separated and collected in sequence according to their different chest width. The operation is simple, the separation speed is fast, and the ratio of separating male pupae can reach over 99%, being a good separation effect.