Abstract: Disclosed herein are in vitro methods of inducing differentiation of human stem cells into spinal GABA interneurons, and spinal dorsal interneurons domain 4 (dl4) progenitors thereof, and cells generated by such methods. The presently disclosed subject matter also provides for uses of such cells for treating spinal cord injury.

Abstract: The present invention provides methods of producing, purifying and expanding mDA progenitor cells.

Abstract: Methods for expanding proliferating populations of neuronal subtype-specific progenitors are provided herein. In particular, the present invention provides methods for maintaining the unique gene profile and differentiation potential of neuronal subtype-specific progenitors, such as motor neuron progenitors and hindbrain serotonergic neural progenitors.

Abstract: A method of differentiating embryonic stem cells into oligodendroglial precursor cells and oligodendroglial cells by culturing a population of cells comprising a majority of cells that are characterized by a neural tube-like rosette morphology and are Pax6+/Sox1+ into a population of cells that are PDGFR?+.

Abstract: A method of differentiating embryonic stem cells into oligodendroglial precursor cells and oligodendroglial cells by culturing a population of cells comprising a majority of cells that are characterized by a neural tube-like rosette morphology and are Pax6+/Sox1+ into a population of cells that are PDGFR?+.

Abstract: A method of differentiating embryonic stem cells into oligodendroglial precursor cells and oligodendroglial cells by culturing a population of cells comprising a majority of cells that are characterized by a neural tube-like rosette morphology and are Pax6+/Sox1+ into a population of cells that are PDGFR?+.

Abstract: An electronic device, includes a USB interface, a processing unit, a master-slave select triggering circuit, a controlling unit, a selection circuit and a switching circuit. The master-slave selection triggering circuit including an input port, a first output port and a second output port. The master-slave select triggering circuit is used to detect the type of external electronic devices connected to the USB interface, if the external electronic device is a master device, the first and second output port output a slave triggering signal, the controlling unit switches off the switching circuit t and the selection circuit selects the processing unit into slave mode; if the external electronic device is the slave device, the first and second output port output a master triggering signal, the controlling unit switches on the switching circuit and the selection circuit select the processing unit into master mode.

Abstract: A method of creating a human pluripotent transgenic stem cell, wherein heterologous DNA is inserted into specific “hot-spots” in the genome where stable and high gene expression may occur, is disclosed. In one embodiment, the method comprises the steps of: (a) selecting a pluripotent stem cell line, and (b) inserting heterologous DNA at an insertion site selected from the group consisting of insertion site one and insertion site two to form a transgenic cell line. In another embodiment, the heterologous DNA is an exchange cassette and the transgenic cell line formed is a master cell line.

Abstract: An electronic device, includes a USB interface, a processing unit, a master-slave select triggering circuit, a controlling unit, a selection circuit and a switching circuit. The master-slave selection triggering circuit including an input port, a first output port and a second output port. The master-slave select triggering circuit is used to detect the type of external electronic devices connected to the USB interface, if the external electronic device is a master device, the first and second output port output a slave triggering signal, the controlling unit switches off the switching circuit t and the selection circuit selects the processing unit into slave mode; if the external electronic device is the slave device, the first and second output port output a master triggering signal, the controlling unit switches on the switching circuit and the selection circuit select the processing unit into master mode.

Abstract: A method of differentiating embryonic stem cells into oligodendroglial precursor cells and oligodendroglial cells by culturing a population of cells comprising a majority of cells that are characterized by a neural tube-like rosette morphology and are Pax6+/Sox1+ into a population of cells that are PDGFR?+.

Abstract: A method of creating a human pluripotent transgenic stem cell, wherein heterologous DNA is inserted into specific “hot-spots” in the genome where stable and high gene expression may occur, is disclosed. In one embodiment, the method comprises the steps of: (a) selecting a pluripotent stem cell line, and (b) inserting heterologous DNA at an insertion site selected from the group consisting of insertion site one and insertion site two to form a transgenic cell line. In another embodiment, the heterologous DNA is an exchange cassette and the transgenic cell line formed is a master cell line.