Patents by Inventor Zhonggang Hou

Zhonggang Hou has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • RNA-DIRECTED DNA CLEAVAGE AND GENE EDITING BY CAS9 ENZYME FROM NEISSERIA MENINGITIDIS
    Publication number: 20240067982
    Abstract: Disclosed are components and methods for RNA-directed DNA cleavage and gene editing. The components include and the methods utilize a Cas9 protein from Neisseria and one or more RNA molecules in order to direct the Cas9 protein to bind to and optionally cleave or nick a target sequence.
    Type: Application
    Filed: November 23, 2022
    Publication date: February 29, 2024
    Inventors: Erik J. Sontheimer, Yan Zhang, Alfonzo Mondragon, Rakhi Rajan, James Thomson, Zhonggang Hou
  • TYPE I-C CRISPR SYSTEM FROM NEISSERIA LACTAMICA AND METHODS OF USE
    Publication number: 20230287457
    Abstract: The disclosure provides a method of altering a DNA sequence, which method comprises the use of a type I-C CRISPR-Cas system based on Neisseria lactamica. The system involves a combination of two or more Neisseria lactamica proteins selected from Cas3, Cas5, Cas8c, and Cas7 and a synthetic guide RNA sequence.
    Type: Application
    Filed: May 26, 2021
    Publication date: September 14, 2023
    Inventors: Yan Zhang, Ailong Ke, Ryan Krueger, Zhonggang Hou
  • TYPE I CRISPR SYSTEM AS A TOOL FOR GENOME EDITING
    Publication number: 20220315920
    Abstract: Provided are compositions, methods, and kits for CRISPR-based editing of DNA targets by Type I CRISPR-associated (Cas) enzymes.
    Type: Application
    Filed: June 21, 2019
    Publication date: October 6, 2022
    Inventors: Ailong KE, Adam DOLAN, Yan ZHANG, Zhonggang HOU
  • DERIVATION OF HUMAN MICROGLIA FROM PLURIPOTENT STEM CELLS
    Publication number: 20220033773
    Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.
    Type: Application
    Filed: August 18, 2021
    Publication date: February 3, 2022
    Inventors: James A. Thomson, Nicholas E. Propson, Michael P. Schwartz, Zhonggang Hou, Gene I. Uenishi, Igor I Slukvin, William L. Murphy, Jue Zhang
  • Human Pluripotent Stem Cell-Based Models for Predictive Developmental Neural Toxicity
    Publication number: 20220017873
    Abstract: The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.
    Type: Application
    Filed: July 12, 2021
    Publication date: January 20, 2022
    Inventors: James A. Thomson, William L. Murphy, Charles D. Page, Michael P. Schwartz, Zhonggang Hou
  • Derivation of human microglia from pluripotent stem cells
    Patent number: 11124765
    Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.
    Type: Grant
    Filed: September 21, 2018
    Date of Patent: September 21, 2021
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: James A. Thomson, Nicholas E. Propson, Michael P. Schwartz, Zhonggang Hou, Gene I. Uenishi, Igor I. Slukvin, William L. Murphy, Jue Zhang
  • Human pluripotent stem cell-based models for predictive developmental neural toxicity
    Patent number: 11060066
    Abstract: The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.
    Type: Grant
    Filed: December 31, 2015
    Date of Patent: July 13, 2021
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: James A. Thomson, William L. Murphy, Charles D. Page, Michael P. Schwartz, Zhonggang Hou
  • DERIVATION OF HUMAN MICROGLIA FROM PLURIPOTENT STEM CELLS
    Publication number: 20190078054
    Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.
    Type: Application
    Filed: September 21, 2018
    Publication date: March 14, 2019
    Inventors: James A. Thomson, Nicholas E. Propson, Michael P. Schwartz, Zhonggang Hou, Gene I. Uenishi, Igor I. Slukvin, William L. Murphy, Jue Zhang
  • Derivation of human microglia from pluripotent stem cells
    Patent number: 10081792
    Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.
    Type: Grant
    Filed: December 31, 2015
    Date of Patent: September 25, 2018
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: James A. Thomson, Nicholas E. Propson, Michael P. Schwartz, Zhonggang Hou, Gene I. Uenishi, Igor I. Slukvin, William L. Murphy, Jue Zhang
  • Vitronectin-derived cell culture substrate for maintaining human pluripotent stem cells in a substantially undifferentiated state
    Patent number: 9771556
    Abstract: Vitronectin-derived cell culture substrates and methods of using the same for culturing pluripotent stem cells are presented. Also provided herein are defined culture systems for maintaining human pluripotent stem cells in a substantially undifferentiated state, where the defined culture system comprises human pluripotent stem cells, a defined culture medium, and at least one polypeptide selected from the group consisting of residues 43 to 378 of SEQ ID NO:1 and residues 45 to 378 of SEQ ID NO:1, and where the defined culture medium comprises insulin, selenium, transferrin, L-ascorbic acid, FGF2, DMEM/F12, NaHCO3, and one of TGF? and NODAL.
    Type: Grant
    Filed: August 5, 2015
    Date of Patent: September 26, 2017
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: James A. Thomson, Zhonggang Hou, Guokai Chen
  • DERIVATION OF HUMAN MICROGLIA FROM PLURIPOTENT STEM CELLS
    Publication number: 20160186137
    Abstract: The present invention relates to methods for deriving human hematopoietic progenitors, primitive macrophages, and microglial cells from human pluripotent stem cells. In particular, provided herein are highly efficient and reproducible methods of obtaining human primitive macrophages and microglia from human pluripotent stem cells, where the primitive macrophages and microglia can be suitable for clinically relevant therapeutic applications.
    Type: Application
    Filed: December 31, 2015
    Publication date: June 30, 2016
    Inventors: James A. Thomson, Nicholas E. Propson, Michael P. Schwartz, Zhonggang Hou, Gene I. Uenishi, Igor I. Slukvin, William L. Murphy, Jue Zhang
  • HUMAN PLURIPOTENT STEM CELL-BASED MODELS FOR PREDICTIVE DEVELOPMENTAL NEURAL TOXICITY
    Publication number: 20160186146
    Abstract: The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.
    Type: Application
    Filed: December 31, 2015
    Publication date: June 30, 2016
    Inventors: James A. Thomson, William L. Murphy, Charles D. Page, Michael P. Schwartz, Zhonggang Hou
  • VITRONECTIN-DERIVED CELL CULTURE SUBSTRATE AND USES THEREOF
    Publication number: 20150368610
    Abstract: Vitronectin-derived cell culture substrates and methods of using the same for culturing pluripotent stem cells are presented.
    Type: Application
    Filed: August 5, 2015
    Publication date: December 24, 2015
    Inventors: James A. Thomson, Zhonggang Hou, Guokai Chen
  • Vitronectin-derived cell culture substrate and uses thereof
    Patent number: 9133266
    Abstract: Vitronectin-derived cell culture substrates and methods of using the same for culturing pluripotent stem cells are presented.
    Type: Grant
    Filed: May 4, 2012
    Date of Patent: September 15, 2015
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: James A. Thomson, Zhonggang Hou, Guokai Chen
  • RNA-DIRECTED DNA CLEAVAGE AND GENE EDITING BY CAS9 ENZYME FROM NEISSERIA MENINGITIDIS
    Publication number: 20140349405
    Abstract: Disclosed are components and methods for RNA-directed DNA cleavage and gene editing. The components include and the methods utilize a Cas9 protein from Neisseria and one or more RNA molecules in order to direct the Cas9 protein to bind to and optionally cleave or nick a target sequence.
    Type: Application
    Filed: May 22, 2014
    Publication date: November 27, 2014
    Applicants: WISCONSIN ALUMNI RESEARCH FOUNDATION, NORTHWESTERN UNIVERSITY
    Inventors: Erik J. Sontheimer, Yan Zhang, Alfonso Mondragon, Rakhi Rajan, James Thomson, Zhonggang Hou
  • Thermostable FGF-2 mutant having enhanced stability
    Patent number: 8772460
    Abstract: Thermostable FGF-2 proteins having enhanced ability to support human pluripotent stem cell cultures are provided. Also provided are methods and compositions utilizing thermostable FGF-2 proteins.
    Type: Grant
    Filed: December 17, 2012
    Date of Patent: July 8, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Guokai Chen, James A. Thomson, Zhonggang Hou
  • FGF-2 HAVING ENHANCED STABILITY
    Publication number: 20130236959
    Abstract: Thermostable FGF-2 proteins having enhanced ability to support human pluripotent stem cell cultures are provided. Also provided are methods and compositions utilizing thermostable FGF-2 proteins.
    Type: Application
    Filed: December 17, 2012
    Publication date: September 12, 2013
    Inventors: Guokai Chen, James A. Thomson, Zhonggang Hou
  • VITRONECTIN-DERIVED CELL CULTURE SUBSTRATE AND USES THEREOF
    Publication number: 20120301962
    Abstract: Vitronectin-derived cell culture substrates and methods of using the same for culturing pluripotent stem cells are presented.
    Type: Application
    Filed: May 4, 2012
    Publication date: November 29, 2012
    Inventors: James A. Thomson, Zhonggang Hou, Guokai Chen