Abstract: The invention discloses a rice temperature sensitive male sterile gene mutant tms18 and its uses. During the study of TMS18 gene, the inventors found that the changing of fertility was related to the structural integrity of the second layer of pollen outer wall. The inventors accidentally found the thermo sensitive male sterile mutant tms18. The fertility of the mutant was affected by different temperatures and can be restored under low temperature treatment. More importantly, the fertility sensitive period of the mutant was different from that of other thermo sensitive male sterile genes. This unique feature of the gene can provide a new theoretical basis and apply value for two-line hybrid rice breeding.

Abstract: The invention discloses a plant thermo-sensitive male sterile gene mutant tms15 and uses thereof. The nucleotide sequence of the plant thermo-sensitive male sterile gene mutant tms15 is SEQ ID No. 1. The inventor screened the thermo sensitive genic male sterile mutant tms15 by EMS mutagenesis of Zhonghua 11, which showed a male sterile phenotype under high temperature conditions (>28° C.) and restored fertility under low temperature conditions (22˜24° C.). The cytological results shows that the sporogenous cells in tms15 plants proliferated excessively and the tapetum cells are also vacuolized abnormally under high temperature. The normal proliferation of sporogenous cells and the normal development of tapetal cells are restored to some extent after low temperature treatment.

Abstract: The invention discloses a rice temperature sensitive male sterile gene mutant tms18 and its uses. During the study of TMS18 gene, the inventors found that the changing of fertility was related to the structural integrity of the second layer of pollen outer wall. The inventors accidentally found the thermo sensitive male sterile mutant tms18. The fertility of the mutant was affected by different temperatures and can be restored under low temperature treatment. More importantly, the fertility sensitive period of the mutant was different from that of other thermo sensitive male sterile genes. This unique feature of the gene can provide a new theoretical basis and apply value for two-line hybrid rice breeding.