Patents by Inventor Zhongping DENG

Zhongping DENG has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240368714
    Abstract: The present application belongs to the field of molecular biology detection, in particular, relates to detection of HIV-1. The present application further provides a kit comprising the composition of the present application, use of the composition and a method for detecting HIV-1. Using the composition of the present application, HIV-1 can be detected with a sensitivity of 22 IU/ml. Meanwhile, in the composition of the present application, two sets of primers and probes for two different regions and an additional forward primer are combined to prepare a reaction system and the HIV-1 RNA are detected using the reaction system to identify the positive reactions, effectively reducing the probability of false negative, especially, of group O viruses. There is no need for a fully enclosed diagnostic system, which effectively reduces costs.
    Type: Application
    Filed: July 18, 2024
    Publication date: November 7, 2024
    Inventors: Lizhong DAI, Feng DING, Xiaoming LIU, Xiaomei REN, Jia LIU, Zhongping DENG
  • Publication number: 20230374615
    Abstract: The disclosure relates to the field of molecular biological detection, specifically, to the field of detection of pathogens that cause respiratory tract infections. More specifically, the disclosure is capable of simultaneously detecting Influenza A virus, Influenza B virus, Rhinovirus, Respiratory adenovirus, Respiratory syncytial virus and Mycoplasma pneumoniae. Using the compositions of the disclosure, it is possible to detect six pathogens that cause respiratory tract infections at the same time and to identify specifically which pathogen(s) causes the infections. Also, it has a higher detectable rate than that of the existing composition, and is more accurate in detection.
    Type: Application
    Filed: June 12, 2023
    Publication date: November 23, 2023
    Inventors: Lizhong DAI, Xiaoliang CHEN, Xiaomei REN, Zhongping DENG, Jia LIU
  • Publication number: 20230287524
    Abstract: Provided is a composition for detecting novel coronavirus 2019-nCoV, influenza A virus, influenza B virus, respiratory adenovirus, respiratory syncytial virus, and Mycoplasma pneumoniae. Also provided are a kit containing the composition, a use of the composition, and a method for detecting and typing pathogens that cause a respiratory infection.
    Type: Application
    Filed: October 11, 2022
    Publication date: September 14, 2023
    Inventors: Lizhong DAI, Xiaoliang CHEN, Xiaomei REN, Jiangyan XIANG, Wang WAN, Shiliu LIAO, Zhongping DENG
  • Publication number: 20230220452
    Abstract: Provided is a composition for detecting mutations of 2019 novel coronavirus mutation. Also provided are a kit containing the composition, the use of the composition, and a method for detecting mutations of 2019 novel coronavirus.
    Type: Application
    Filed: August 18, 2022
    Publication date: July 13, 2023
    Inventors: Lizhong DAI, Zhongping DENG, Jin YAN, Shiyao CHEN, Deyong TAN, Jia LIU, Xiaomei REN, Xinwu GUO
  • Publication number: 20230193407
    Abstract: Provided in the present invention is a composition capable of detecting and typing novel coronavirus 2019-nCoV, coronavirus 229E, coronavirus NL63, coronavirus OC43, coronavirus HKU1, coronavirus MERSr-CoV, and coronavirus SARSr-CoV. At the same time, further provided is a kit comprising the composition and a method for detecting and typing coronaviruses. The composition of the present invention in combination with a fluorescent probe method and a melting curve method can perform simultaneous detection and typing of seven coronaviruses in one tube.
    Type: Application
    Filed: August 19, 2022
    Publication date: June 22, 2023
    Inventors: Lizhong DAI, Deyong TAN, Zhongping DENG, Jia LIU, Bozhi JI, Xing CHENG, Qingzhi SUN, Jin YAN
  • Publication number: 20230193369
    Abstract: A composition for improving the detection performance of fluorescent quantitative PCR. The composition comprises bovine serum albumin, sorbitol, ammonium sulfate, formamide, tetramethylammonium chloride, and at least one of dithiothreitol and betaine. The present invention further relates to a qPCR reaction liquid containing the composition and a preparation method therefor. The composition can improve the sensitivity, specificity, and interference resistance of real-time fluorescent quantitative PCR.
    Type: Application
    Filed: September 28, 2022
    Publication date: June 22, 2023
    Inventors: Lizhong DAI, Bozhi JI, Kang WU, Jia LIU, Zhongping DENG, Weimin MIAO
  • Publication number: 20230183824
    Abstract: Provided is a composition for detecting a novel coronavirus, influenza A virus and influenza B virus; moreover, also provided are a kit including the composition, an application of the kit, and a method for detecting and typing viruses causing respiratory tract infection.
    Type: Application
    Filed: September 19, 2022
    Publication date: June 15, 2023
    Inventors: Lizhong DAI, Deyong TAN, Zhongping DENG, Jia LIU, Qingzhi SUN, Xing CHENG, Xiaomei REN, Xiaoliang CHEN, Junjie MAO
  • Publication number: 20230090551
    Abstract: A composition for detecting SARS-CoV-2 is provided; moreover, a kit including the composition, the use of the kit, and a method for detecting SARS-CoV-2 are also provided.
    Type: Application
    Filed: August 26, 2022
    Publication date: March 23, 2023
    Inventors: Lizhong DAI, Zhongping DENG, Deyong TAN, Jia LIU, Bozhi JI, Jin YAN, Xinwu GUO, Xiaomei REN, Kang WU, Xing CHENG
  • Publication number: 20230035871
    Abstract: The present invention relates to the field of molecular biology detection, more particularly to a method for fluorescent quantitative PCR. The method includes: 1) mixing an upstream and downstream primer pair, a fluorescent probe, and a PCR amplification reagent; and 2) carrying out the fluorescent quantitative PCR, where the fluorescent probe has two quenching groups, in which a first quenching group is located at a 3? end and a second quenching group is labeled on a T base and is 10-15 nt apart from the first quenching group. Using the method for fluorescent quantitative PCR, background signals in the fluorescent quantitative PCR can be reduced; furthermore, the sensitivity of PCR can be improved, the occurrence of false negatives in detection can be reduced, and the amplification efficiency of the fluorescent quantitative PCR can also be improved.
    Type: Application
    Filed: August 19, 2022
    Publication date: February 2, 2023
    Inventors: Lizhong DAI, Bozhi JI, Deyong TAN, Jia LIU, Xu FAN, Zhongping DENG
  • Publication number: 20220411852
    Abstract: Provided is a composition for detecting multiple encephalitis/meningitis/respiratory pathogens. The composition includes primer sequences for the pathogens as shown in SEQ ID NOs: 1-7, 9-23 and 25-32, in which SEQ ID NOs: 1-7 and 9-16 carry fluorescent reporter groups. In addition, the present invention further provides a use of the foregoing composition in the preparation of a kit, and a related kit and a method for using the same.
    Type: Application
    Filed: September 2, 2022
    Publication date: December 29, 2022
    Inventors: Lizhong Dai, Deyong TAN, Zhongping DENG, Jia LIU, Xiaomei REN, Xing CHENG, Qingzhi SUN
  • Publication number: 20220389482
    Abstract: The present invention relates to the field of viral nucleic acid detection. In particular, the present invention provides a pretreatment method for viral nucleic acid detection. The method includes mixing a pretreatment solution containing a sample with a nucleic acid releasing agent and a qPCR amplification reagent, wherein the pretreatment solution includes Tris-HCl, EDTA-2Na, sodium chloride, a ribonuclease (RNase) inhibitor, and an antibiotic; and the pretreatment solution has a pH of 6.5-8.0.
    Type: Application
    Filed: August 19, 2022
    Publication date: December 8, 2022
    Inventors: Lizhong DAI, Bozhi JI, Zhongping DENG, Jia LIU, Deyong TAN, Xu FAN
  • Publication number: 20220064706
    Abstract: A nucleic acid release agent, a PCR amplification method and a PCR amplification kit are provided. The nucleic acid release agent includes Tris-HCl, sodium chloride, potassium chloride, tween 20, Triton X-100, ethyl phenyl polyethylene glycol and a strong base; wherein the molar concentration of Tris-HCl is 0.5 mM to 500 mM, the molar concentration of sodium chloride is 20 mM to 500 mM, the volume percentage of Tween 20 is 0.1% to 2%, the volume percentage of Triton X-100 is 0.1% to 3%, the volume percentage of ethyl phenyl polyethylene glycol is 0.1% to 3%, the mass concentration of potassium chloride is 5 mg/mL to 8 mg/mL and the mass concentration of the strong base is 2 mg/mL to 50 mg/mL.
    Type: Application
    Filed: April 29, 2019
    Publication date: March 3, 2022
    Applicant: SANSURE BIOTECH INC.
    Inventors: Lizhong DAI, Bozhi JI, Wenqu ZHANG, Zhongping DENG, Wenzhou FAN