Patents by Inventor Zhongsen Li
Zhongsen Li has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11555197Abstract: The invention provides a compositions and methods for controlling phenotypic traits in plants. Genes of interest are placed under the control of a gene switch to allow inducible control or expression of a gene of interest “on-demand” by treatment of the plant with a chemical ligand.Type: GrantFiled: July 25, 2017Date of Patent: January 17, 2023Assignee: GREENVENUS, LLCInventors: Sekhar Boddupalli, Andrey Boukharov, Rio Stamler, Zhongsen Li, Arianne Tremblay, Stephen Schauer, Shiv B. Tiwari, John Salmeron
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Patent number: 11459576Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.Type: GrantFiled: July 10, 2020Date of Patent: October 4, 2022Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael Lassner, Derek Jantz, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev, James Jefferson Smith
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Patent number: 11427830Abstract: The disclosure relates to gene expression regulatory sequences, specifically to the promoter of a U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.Type: GrantFiled: August 27, 2019Date of Patent: August 30, 2022Assignee: E. I. DU PONT DE NEMOURS AND COMPANYInventors: Zhongsen Li, Zhan-Bin Liu
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Publication number: 20220251586Abstract: Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.Type: ApplicationFiled: March 21, 2022Publication date: August 11, 2022Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: SAVERIO CARL FALCO, MICHAEL LASSNER, ZHONGSEN LI, CHRISTOPHER J SCELONGE
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Patent number: 11312969Abstract: Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.Type: GrantFiled: August 26, 2019Date of Patent: April 26, 2022Inventors: Saverio Carl Falco, Michael Lassner, Zhongsen Li, Christopher J Scelonge
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Publication number: 20210324482Abstract: Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.Type: ApplicationFiled: June 25, 2021Publication date: October 21, 2021Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: DAVID GEORGE CHARNE, WENPIN CHEN, CHADWICK BRUCE KOSCIELNY, ZHONGSEN LI, JAYANTILAL DEVABHAI PATEL, FERDINAND THOONEN, LOMAS TULSIERAM, YONGPING ZHANG
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Patent number: 11078546Abstract: Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.Type: GrantFiled: June 15, 2017Date of Patent: August 3, 2021Assignees: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: David George Charne, Wenpin Chen, Chadwick Bruce Koscielny, Zhongsen Li, Jayantilal Devabhai Patel, Ferdinand Thoonen, Lomas Tulsieram, Yongping Zhang
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Publication number: 20210062201Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.Type: ApplicationFiled: July 10, 2020Publication date: March 4, 2021Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, MICHAEL LASSNER, DEREK JANTZ, ZHONGSEN LI, ZHAN-BIN LIU, SERGEI SVITASHEV, JAMES JEFFERSON SMITH
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Patent number: 10870859Abstract: The disclosure relates to gene expression regulatory sequences from maize, specifically to the promoter of a maize U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.Type: GrantFiled: November 5, 2019Date of Patent: December 22, 2020Inventors: Zhongsen Li, Zhan-Bin Liu
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Patent number: 10822610Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.Type: GrantFiled: June 13, 2018Date of Patent: November 3, 2020Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael Lassner, Derek Jantz, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev, James Jefferson Smith
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Publication number: 20200157554Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.Type: ApplicationFiled: January 30, 2020Publication date: May 21, 2020Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
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Publication number: 20200063151Abstract: The disclosure relates to gene expression regulatory sequences, specifically to the promoter of a U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.Type: ApplicationFiled: November 5, 2019Publication date: February 27, 2020Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: ZHONGSEN LI, ZHAN-BIN LIU
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Patent number: 10519457Abstract: The disclosure relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.Type: GrantFiled: August 20, 2014Date of Patent: December 31, 2019Assignee: E I DU PONT DE NEMOURS AND COMPANYInventors: Zhongsen Li, Zhan-Bin Liu
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Publication number: 20190376073Abstract: Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.Type: ApplicationFiled: August 26, 2019Publication date: December 12, 2019Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: SAVERIO CARL FALCO, MICHAEL LASSNER, ZHONGSEN LI, CHRISTOPHER J SCELONGE
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Publication number: 20190376074Abstract: The disclosure relates to gene expression regulatory sequences, specifically to the promoter of a U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.Type: ApplicationFiled: August 27, 2019Publication date: December 12, 2019Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: ZHONGSEN LI, ZHAN-BIN LIU
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Publication number: 20190367934Abstract: Methods for the identification of variant recognition sites for rare cutting engineered double strand break inducing agents and compositions thereof are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the of variant recognition sites. Various methods of identifying variant recognition sites with increased substrate activity for a rare cutting engineered double strand break inducing agents are provided.Type: ApplicationFiled: May 17, 2019Publication date: December 5, 2019Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: STEPHANE DESCHAMPS, JAMES ENGLISH, ZHONGSEN LI, JOSHUA K. YOUNG, VICTOR LLACA
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Patent number: 10450576Abstract: The invention relates to gene expression regulatory sequences from soybean, specifically to promoters of U6 small nuclear RNA genes, and fragments thereof, and their use in promoting the expression of one or more heterologous nucleic acid fragments in a constitutive manner in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.Type: GrantFiled: March 21, 2016Date of Patent: October 22, 2019Assignee: E I DU PONT DE NEMOURS AND COMPANYInventor: Zhongsen Li
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Patent number: 10435699Abstract: Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.Type: GrantFiled: January 24, 2013Date of Patent: October 8, 2019Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Saverio Carl Falco, Michael W Lassner, Zhongsen Li, Christopher Jay Scelonge
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Publication number: 20190233834Abstract: The invention provides a compositions and methods for controlling phenotypic traits in plants. Genes of interest are placed under the control of a gene switch to allow inducible control or expression of a gene of interest “on-demand” by treatment of the plant with a chemical ligand.Type: ApplicationFiled: July 25, 2017Publication date: August 1, 2019Applicant: INTREXON CORPORATIONInventors: Sekhar BODDUPALLI, Andrey BOUKHAROV, Rio STAMLER, Zhongsen LI, Arianne TREMBLAY, Stephen SCHAUER, Shiv B. TIWARI, John SALMERON
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Patent number: 10329574Abstract: Methods for the identification of variant recognition sites for rare cutting engineered double strand break inducing agents and compositions thereof are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the of variant recognition sites. Various methods of identifying variant recognition sites with increased substrate activity for a rare cutting engineered double strand break inducing agents are provided.Type: GrantFiled: March 10, 2014Date of Patent: June 25, 2019Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Stephane Deschamps, James English, Zhongsen Li, Victor Llaca, Joshua K. Young