Abstract: The present invention relates to artificially synthesized modified mono-methylated lysines and modified mono-methylated lysine derivatized polypeptides. The present invention also relates to production of a completely new antibody by using this modified mono-methylated lysine and modified mono-methylated lysine derivatized polypeptide as an antigen, this antibody can be used in recognizing and enriching the modified polypeptide after the lysine mono-methylated polypeptide being derivatized in vitro. By using this antibody, it is capable of detecting whether a mono-methylation modification is present in amino acids on the polypeptide sequence, the present invention also relates to a preparation method of said antibody.

Abstract: The disclosure discloses a mutant of nitrile hydratase derived from Caldalkalibacillus thermarum, and belongs to the technical field of enzyme engineering. The nitrile hydratase mutant Cal. t Nhase-A20V provided by the disclosure has a half-life of about 10 min at 70° C., which does not change much compared with the thermal stability of the wild enzyme. The specific enzyme activity of the mutant Cal. t Nhase-A20V is 128% of that of the wild enzyme. At the same time, the mutant also has better tolerance to a substrate and a product, and the final yield of nicotinamide produced by whole-cell catalysis reaches 598 g/L. Therefore, the nitrile hydratase mutant Cal. t Nhase-A20V provided by the disclosure has good enzymatic properties and is beneficial to future industrial production.

Abstract: The invention provides an isolated peptide comprising a crotonylation site, a Kcr-specific affinity reagent that specifically binds to the peptide, and a method for detecting protein crotonylation in a sample using the reagent.

Abstract: The present invention relates to artificially synthesized modified mono-methylated lysines and modified mono-methylated lysine derivatized polypeptides. The present invention also relates to production of a completely new antibody by using this modified mono-methylated lysine and modified mono-methylated lysine derivatized polypeptide as an antigen, this antibody can be used in recognizing and enriching the modified polypeptide after the lysine mono-methylated polypeptide being derivatized in vitro. By using this antibody, it is capable of detecting whether a mono-methylation modification is present in amino acids on the polypeptide sequence, the present invention also relates to a preparation method of said antibody.

Abstract: Provided are artificially synthesized modified mono-methylated lysines and modified mono-methylated lysine derivatized polypeptides. Also provided is an antibody produced by using this modified mono-methylated lysine and modified monomethylated lysine derivatized polypeptide as an antigen, the antibody can be used in recognizing and enriching the modified polypeptide after the lysine mono-methylated polypeptide being derivatized in vitro. Also provided are a preparation method of said antibody, a method for identifying and quantifying the lysine mono-methylated modified substrate in cell or tissue by using proteomics approach of affinity enrichment for specific antibody and mass spectrometry.

Abstract: The invention provides an isolated peptide comprising a crotonylation site, a Kcr-specific affinity reagent that specifically binds to the peptide, and a method for detecting protein crotonylation in a sample using the reagent.

Abstract: The present disclosure includes compositions and methods of making a nanoparticle composition comprising a phospholipids core comprising one or more lipids and one or more active agents, and at least one layer of one or more polymers on the surface of the phospholipids core; more specifically, the disclosure relates to the use of capecitabine (N4-pentyloxycarbonyl-5-deoxy-5-fluoro-cytidine, CAP) within such a lipid-polymer nanoparticle formulation for optimizing pharmaceutical properties of capecitabine for the treatment of cancer.

Abstract: Provided are artificially synthesized modified mono-methylated lysines and modified mono-methylated lysine derivatized polypeptides. Also provided is an antibody produced by using this modified mono-methylated lysine and modified monomethylated lysine derivatized polypeptide as an antigen, the antibody can be used in recognizing and enriching the modified polypeptide after the lysine mono-methylated polypeptide being derivatized in vitro. Also provided are a preparation method of said antibody, a method for identifying and quantifying the lysine mono-methylated modified substrate in cell or tissue by using proteomics approach of affinity enrichment for specific antibody and mass spectrometry.

Abstract: The invention provides an isolated peptide comprising a crotonylation site, a Kcr-specific affinity reagent that specifically binds to the peptide, and a method for detecting protein crotonylation in a sample using the reagent.