Abstract: The present invention discloses an improved method for magnetic transfection of maize pollen, including: (1) preparing plasmid DNA for transfection; (2) binding MNP nanomagnetic beads to the plasmid DNA at room temperature to form MNP-DNA complex; (3) collecting fresh pollen from maize at full flowering and bringing it rapidly back indoors under ice box storage conditions insulated from water; (4) mixing a maize pollen transformation solution with sieved maize pollen and being subjected to aperture-opening pre-treatment at low temperature; (5) adding the MNP-DNA complex to aperture-opening pre-treatment solution, mixing gently and placing on a low temperature pre-cooled magnetic plate for transfection; (6) after transfection, taking the pollen suspension to field in ice box and pollinating directly on ears of maize.

Abstract: The present invention discloses a method for site-directed modification of whole plant through gene transient expression. The method as provided for conducting site-directed modification to a target fragment of a target gene in a whole plant comprises the following steps: transiently expressing a sequence-specific nuclease in said plant, wherein a whole plant is used as the subject for transient expression, said sequence-specific nuclease targets and cleaves said target fragment, thereby the site-directed modification is achieved via the self DNA repairing of said plant.

Abstract: The present invention discloses a method for site-directed modification of whole plant through gene transient expression. The method as provided for conducting site-directed modification to a target fragment of a target gene in a whole plant comprises the following steps: transiently expressing a sequence-specific nuclease in said plant, wherein a whole plant is used as the subject for transient expression, said sequence-specific nuclease targets and cleaves said target fragment, thereby the site-directed modification is achieved via the self DNA repairing of said plant.

Abstract: A sample transport method, applied to a test instrument including a conveyor belt, and a loading platform and a grab position sequentially disposed along a transport direction of the conveyor belt, wherein a plurality of sample holder transport positions are disposed on the conveyor belt; the method including: pushing a first sample holder from the loading platform to a sample holder transport position of the conveyor; when a sample position on the first sample holder moves to the grab position, determining whether the sample position moved the grab position is a target sample position; if so, then determining whether all samples positions on the first sample holder before the target sample holder other than a predetermined number of sample positions have completed testing, wherein the predetermined number is sample position number corresponding to a delay time in outputting information indicating whether a sample needs to be retested.

Abstract: A method for synchronizing list-mode data of single events in a PET imaging includes: acquiring list-mode data of single events of each independent detector module, calculating a probability density of time intervals between occurrences of single events in detector module and setting initial parameters, determining detection starting time difference of each detector module with iterative peak searching and graded time window, and performing synchronization correction and coincidence discrimination on the single event data in each detector module based on the detection starting time difference. A system for synchronizing list-mode data of single events in a PET imaging includes: a data acquisition and frequency difference compensation module, an initial parameter setting module, a coarse time scale coincidence module, a fine time scale coincidence module and a data synchronization correction and coincidence discrimination module.

Abstract: Provided is a plasmid library comprising a DNA insertion site and two barcode sequences located upstream and downstream of the site. The combinations of two barcode sequences of any two plasmids selected from the library are different. Also provided is a method for high-throughput paired-end sequencing of an inserted DNA using the plasmid library.

Abstract: A sample transport method, applied to a test instrument including a conveyor belt, and a loading platform and a grab position sequentially disposed along a transport direction of the conveyor belt, wherein a plurality of sample holder transport positions are disposed on the conveyor belt; the method including: pushing a first sample holder from the loading platform to a sample holder transport position of the conveyor; when a sample position on the first sample holder moves to the grab position, determining whether the sample position moved the grab position is a target sample position; if so, then determining whether all samples positions on the first sample holder before the target sample holder other than a predetermined number of sample positions have completed testing, wherein the predetermined number is sample position number corresponding to a delay time in outputting information indicating whether a sample needs to be retested.

Abstract: The present invention discloses a method for site-directed modification of whole plant through gene transient expression. The method as provided for conducting site-directed modification to a target fragment of a target gene in a whole plant comprises the following steps: transiently expressing a sequence-specific nuclease in said plant, wherein a whole plant is used as the subject for transient expression, said sequence-specific nuclease targets and cleaves said target fragment, thereby the site-directed modification is achieved via the self DNA repairing of said plant.

Abstract: A quartz watch movement includes a minute wheel connected to a handsetting wheel by an intermediate wheel, a shaft of a third wheel placed in a shaft hole in a main plate, elastic connection structures being rotated by a third-wheel shaft gear and a minute wheel piece gear which are engaged together and are arranged between a minute wheel tube and the minute wheel piece gear when a stem is pulled or the minute hand is adjusted through the handsetting wheel, the intermediate wheel and a minute wheel shaft gear, and a second-hand stop lever detachably connected to a second-hand stop electrode. The elastic connection structures are arranged between the minute wheel tube and the minute wheel piece gear, so that the quartz watch is accurate in travel time and has the function of efficiently adjusting the hour hand and the minute hand when omitting a handsetting middle wheel.

Abstract: A quartz watch movement includes a minute wheel connected to a handsetting wheel by an intermediate wheel, a shaft of a third wheel placed in a shaft hole in a main plate, elastic connection structures being rotated by a third-wheel shaft gear and a minute wheel piece gear which are engaged together and are arranged between a minute wheel tube and the minute wheel piece gear when a stem is pulled or the minute hand is adjusted through the handsetting wheel, the intermediate wheel and a minute wheel shaft gear, and a second-hand stop lever detachably connected to a second-hand stop electrode. The elastic connection structures are arranged between the minute wheel tube and the minute wheel piece gear, so that the quartz watch is accurate in travel time and has the function of efficiently adjusting the hour hand and the minute hand when omitting a handsetting middle wheel.

Abstract: A method for synchronizing list-mode data of single events in a PET imaging includes: acquiring list-mode data of single events of each independent detection module, calculating a probability density of time intervals between occurrences of single events in detection module and setting initial parameters, determining detection starting time difference of each detection module with iterative peak searching and graded time window, and performing synchronization correction and coincidence discrimination on the single event data in each detection module based on the detection starting time difference. A system for synchronizing list-mode data of single events in a PET imaging includes: a data acquisition and frequency difference compensation module, an initial parameter setting module, a coarse time scale coincidence module, a fine time scale coincidence module and a data synchronization correction and coincidence discrimination module.