Abstract: This disclosure provides methods, kits, and compositions to amplify sample DNA of low quantity by adding an amount of buffer DNA before amplification. The method protects the sample DNA against degradation and PCR bias. The method disclosed herein are useful to amplify DNA of low amount, such as genomic DNA from a single cell and therefore are especially useful for genetic diagnostic tests and precision medicine.

Abstract: This disclosure provides methods, kits, and compositions to amplify sample DNA of low quantity by adding an amount of buffer DNA before amplification. The method protects the sample DNA against degradation and PCR bias. The method disclosed herein are useful to amplify DNA of low amount, such as genomic DNA from a single cell and therefore are especially useful for genetic diagnostic tests and precision medicine.

Abstract: This disclosure provides a droplet thermal cycling reaction (DTCR) device comprising a helical tubing through which a colloid flows; a pump that drives the flow of the colloid; and one or more temperature control sheets (TCS). The pump is configured to drive the colloid to flow through the helical tubing. Optionally, the pump is connected to either the inlet or the outlet of the helical tubing. The TCS sheets, configured to control temperatures for reactions occurring on the device, can be placed either outside or inside helical tubing, and contain at least two temperature zones so that the colloid flows through the different temperature zones along inside the helical tubing. The DTCR of this disclosure has technical benefits of reducing device complexity, enabling device miniaturization, reducing PCR reaction volume, dropletizing PCR reactions, and reducing the cost of digital PCR.

Abstract: This disclosure provides methods, kits, and compositions to amplify sample DNA of low quantity by adding an amount of buffer DNA before amplification. The method protects the sample DNA against degradation and PCR bias. The method disclosed herein are useful to amplify DNA of low amount, such as genomic DNA from a single cell and therefore are especially useful for genetic diagnostic tests and precision medicine.