Abstract: A coal and gas outburst monitoring device includes the insertion and anchoring assembly, and the pump drainage and monitoring mechanism; the insertion and anchoring assembly is vertically inserted and distributed on the sidewall of surrounding rock of coal mine roadway, and the insertion and anchoring assembly can monitor the stress change data in the sidewall of surrounding rock in real time; the pump drainage and monitoring mechanism is erected within the coal mine roadway, and the pump drainage and monitoring mechanism can cooperate with the insertion and anchoring assembly to perform the gas pump drainage monitoring for the sidewall of surrounding rock of goaf, and to obtain the gas seepage monitoring data in the surface layer and the inner layer of sidewall of surrounding rock. Thus, comparing with the database mechanism, the accurate and advanced predictions for the gas outburst can be made.

Abstract: The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

Abstract: The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

Abstract: The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

Abstract: A coal and gas outburst monitoring device includes the insertion and anchoring assembly, and the pump drainage and monitoring mechanism; the insertion and anchoring assembly is vertically inserted and distributed on the sidewall of surrounding rock of coal mine roadway, and the insertion and anchoring assembly can monitor the stress change data in the sidewall of surrounding rock in real time; the pump drainage and monitoring mechanism is erected within the coal mine roadway, and the pump drainage and monitoring mechanism can cooperate with the insertion and anchoring assembly to perform the gas pump drainage monitoring for the sidewall of surrounding rock of goaf, and to obtain the gas seepage monitoring data in the surface layer and the inner layer of sidewall of surrounding rock. Thus, comparing with the database mechanism, the accurate and advanced predictions for the gas outburst can be made.

Abstract: Disclosed are a network optimization method and apparatus, an electronic device, and a storage medium. The network optimization method may include: sending data analytics request information to a core network; receiving data analytics response information fed back by the core network and determining a model processing result according to an artificial intelligence learning model; and performing a network optimization operation according to the model processing result.

Abstract: Equipment for self-positioning handling of aluminum profiles for rail vehicle, including a handling and lifting mechanism, a steel beam fixed on and being driven to lift by the mechanism, 2-DOF spatial mechanisms fixed on the steel beam and mechanical clamping jaws fixed on a clamping jaw connecting seat; the 2-DOF spatial mechanisms includes a cylinder supporting seat, four connecting members on an outer ring of the cylinder supporting seat hinged respectively to four first connecting rods; wherein, two of the first rods are hinged to first ends of two ball joint connecting rods of which second ends are hinged to the clamping jaw connecting seat; the other two first rods are hinged to first ends of two second connecting rods of which second ends are hinged to the clamping jaw connecting seat; a piston rod of a cylinder center of the cylinder supporting seat driving the clamping jaw connecting seat.

Abstract: The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

Abstract: A method of rapid constructing human cerebral cortical organoids by 3D bioprinting and an application including preparing microfluidic chips, preparation of hydrogel of human cerebral cortical organoids, and printing of human cerebral cortical organoids. The microfluidic chip comprises a mixed-flow channel layer, liquid pool layer, microporous array layer, human cerebral cortical organoid culture layer, and culture medium recovery layer; the human cerebral cortical organoid hydrogel has gelatin, alginate, and hyaluronic acid; printing directly human cerebral cortical organoids in microfluidic chips by FRESH printing method, obtaining human cerebral cortical organoid chips after packaging. The application directly constructs large-scale human cerebral cortex-like with three layers of mutually connected structures in situ in organ chip through 3D bioprinting, simulates cerebrospinal fluid circulation through perfusion culture.

Abstract: A hydrogel for cell-laden bioprinting, bioink, and a preparation method and an application thereof, relates to the technical field of biomedical polymer hydrogels. The hydrogel for cell-laden bioprinting is polymer gel formed by adding a cell-specific material into a matrix of alginate and gelatin and crosslinking and curing, wherein the cell-specific material is polypeptide selected according to different laden cells. The structures printed using the hydrogel may have the advantages such as adjustable mechanical properties, adjustable porosity, high biocompatibility, high printing accuracy, and high customizability, which may widely support the printing of human tissues and organs such as spinal cord, cartilage, and heart, and has good prospects for applications in tissue repair, organ transplantation and so on.

Abstract: The present disclosure provides a preparation method of a catalyst slurry for a fuel cell membrane electrode assembly (MEA), including the following steps: preparing a slurry mixture with a catalyst, a dispersing solvent, an ionomer, a thickener, and a surfactant according to a certain mass ratio; subjecting the slurry mixture to pre-dispersion several times in an ultrasonic disperser and a high-shear emulsifying machine successively, to obtain a slurry pre-dispersion; and conducting dispersion on the slurry pre-dispersion in a high-pressure homogenizer to obtain the catalyst slurry. In the present disclosure, components of the catalyst slurry and a dispersion process are optimized and innovated, to construct a more effective three-phase interface. The MEA prepared according to the present disclosure has a significantly improved performance and reduced slurrying time; and is thus suitable for mass production.

Abstract: A card edge connector with an insulative housing includes opposite longitudinal side walls along a longitudinal direction with a receiving slot therebetween in a transverse direction. Each side wall forms a plurality of passageways. A plurality of contacts are respectively received within the corresponding passageways. Each contact includes a retaining section, a contacting section extending from the retaining section into the receiving slot, and a leg extending downwardly from the retaining section out of the housing. Each contact is further equipped with an auxiliary spring beam with an additional contacting section extending into the receiving slot. The contacts are initially linked to a single contact carrier strip so as to be simultaneously inserted into the corresponding passageways, respectively.

Abstract: A card edge connector with an insulative housing includes opposite longitudinal side walls along a longitudinal direction with a receiving slot therebetween in a transverse direction. Each side wall forms a plurality of passageways. A plurality of contacts are respectively received within the corresponding passageways. Each contact includes a retaining section, a contacting section extending from the retaining section into the receiving slot, and a leg extending downwardly from the retaining section out of the housing. Each contact is further equipped with an auxiliary spring beam with an additional contacting section extending into the receiving slot. The contacts are initially linked to a single contact carrier strip so as to be simultaneously inserted into the corresponding passageways, respectively.

Abstract: An electrical card edge connector for receiving an electronic card with a bottom notch therein, includes an insulative housing, a plurality of terminals and an alignment key. The housing includes two opposite side walls, a bottom wall and a central slot among the side walls and the bottom wall. The terminals are disposed in the corresponding side walls and extend into the central slot for mating with the electronic card. The alignment key includes a body received within the central slot, a pair of extension sections covering the two side walls, and a pair of securing sections attached to the bottom wall wherein the body is adapted to be received within the bottom notch of the electronic card.

Abstract: An electrical card edge connector for receiving an electronic card with a bottom notch therein, includes an insulative housing, a plurality of terminals and an alignment key. The housing includes two opposite side walls, a bottom wall and a central slot among the side walls and the bottom wall. The terminals are disposed in the corresponding side walls and extend into the central slot for mating with the electronic card. The alignment key includes a body received within the central slot, a pair of extension sections covering the two side walls, and a pair of securing sections attached to the bottom wall wherein the body is adapted to be received within the bottom notch of the electronic card.

Abstract: An automatic analysis method for operation sequence and a system thereof are disclosed. The method comprising: receiving at least one operation sequence containing at least one operation record, the operation record including an operation of switching from a previous user interface to a post user interface, an interval time of switching from the previous user interface to the post user interface; forming time-dependent operation record groups of respective operation sequences based on the interval time and a first time threshold, wherein the time-dependent operation record group includes operation records whose interval time is less than the first time threshold; comparing time-dependent operation record groups of respective operation sequences to obtain identical time-dependent operation record groups; and calculating a frequency that identical time-dependent operation record groups occur in the operation sequence to obtain the identical time-dependent operation record groups having high frequency.

Abstract: An automatic analysis method for operation sequence and a system thereof are disclosed. The method comprising: receiving at least one operation sequence containing at least one operation record, the operation record including an operation of switching from a previous user interface to a post user interface, an interval time of switching from the previous user interface to the post user interface; forming time-dependent operation record groups of respective operation sequences based on the interval time and a first time threshold, wherein the time-dependent operation record group includes operation records whose interval time is less than the first time threshold; comparing time-dependent operation record groups of respective operation sequences to obtain identical time-dependent operation record groups; and calculating a frequency that identical time-dependent operation record groups occur in the operation sequence to obtain the identical time-dependent operation record groups having high frequency.