Abstract: The present invention generally relates to systems and methods for imaging or determining nucleic acids or other desired targets, for instance, within cells or tissues. In one aspect, a sample is exposed to a plurality of nucleic acid probes that are determined within the sample. In some cases, however, background fluorescence or off-target binding may make it more difficult to determine properly bound nucleic acid probes. Accordingly, other components of the samples that may be contributing to the background, such as proteins, lipids, and/or other non-targets, may be “cleared” from the sample to improve determination. However, in certain embodiments, nucleic acids or other desired targets may be prevented from also being cleared, e.g., using polymers or gels within the sample. Other aspects are generally directed to compositions or kits involving such systems, methods of using such systems, or the like.

Abstract: The present disclosure provides a three-dimensional hydrogel-graphene-based biosensor and a preparation method thereof, belonging to the technical field of biosensors. The present disclosure provides a three-dimensional hydrogel-graphene-based biosensor, including a substrate, an electrode layer, a graphene film, and a three-dimensional hydrogel material layer that are stacked in sequence; where the three-dimensional hydrogel material layer is formed of a hydrogel material having a three-dimensional network structure; the hydrogel material is obtained by polymerization of raw materials including an acrylamide monomer and a modified probe molecule; and the modified probe molecule is a probe molecule modified with an acrylamide group. The three-dimensional hydrogel-graphene-based biosensor has a desirable stability and a high sensitivity.

Abstract: The present disclosure provides a three-dimensional hydrogel-graphene-based biosensor and a preparation method thereof, belonging to the technical field of biosensors. The present disclosure provides a three-dimensional hydrogel-graphene-based biosensor, including a substrate, an electrode layer, a graphene film, and a three-dimensional hydrogel material layer that are stacked in sequence; where the three-dimensional hydrogel material layer is formed of a hydrogel material having a three-dimensional network structure; the hydrogel material is obtained by polymerization of raw materials including an acrylamide monomer and a modified probe molecule; and the modified probe molecule is a probe molecule modified with an acrylamide group. The three-dimensional hydrogel-graphene-based biosensor has a desirable stability and a high sensitivity.

Abstract: A microdevice for monitoring a target analyte is provided. The microdevice can include a field effect transistor comprising a substrate, a gate electrode, and a microfluidic channel including graphene. The microfluidic channel can be formed between drain electrodes and source electrodes on the substrate. The microdevice can also include at least one aptamer functionalized on a surface of the graphene. The at least one aptamer can be adapted for binding to the target analyte. Binding of the target analyte to the at least one aptamer can alter the conductance of the graphene.

Abstract: A microdevice for monitoring a target analyte is provided. The microdevice can include a field effect transistor comprising a substrate, a gate electrode, and a microfluidic channel including graphene. The microfluidic channel can be formed between drain electrodes and source electrodes on the substrate. The microdevice can also include at least one aptamer functionalized on a surface of the graphene. The at least one aptamer can be adapted for binding to the target analyte. Binding of the target analyte to the at least one aptamer can alter the conductance of the graphene.

Abstract: For the present invention, a P-type thermo-electric thin-film layer and a N-type thermo-electric thin-film layer are respectively deposited on two sides of an insulating substrate. During the deposition, the P-type thermo-electric thin-film layer and the N-type thermo-electric thin-film layer are deposited and connected on the same exposed side of the insulating substrate, and then a PN junction is formed. This method makes the fabrication simplified without special process for connecting the P-type thermo-electric thin-film layer and the N-type thermo-electric thin-film layer. Due to the features of thin-film thermo-electric material, the performance of thermo-electric generator is improved. During the deposition, the P-type thermo-electric thin-film layer and the N-type thermo-electric thin-film layer are deposited and connected on the exposed side of the insulating substrate, so welding is not required in this heating surface side.

Abstract: For the present invention, a P-type thermo-electric thin-film layer and a N-type thermo-electric thin-film layer are respectively deposited on two sides of an insulating substrate. During the deposition, the P-type thermo-electric thin-film layer and the N-type thermo-electric thin-film layer are deposited and connected on the same exposed side of the insulating substrate, and then a PN junction is formed. This method makes the fabrication simplified without special process for connecting the P-type thermo-electric thin-film layer and the N-type thermo-electric thin-film layer. Due to the features of thin-film thermo-electric material, the performance of thermo-electric generator is improved. During the deposition, the P-type thermo-electric thin-film layer and the N-type thermo-electric thin-film layer are deposited and connected on the exposed side of the insulating substrate, so welding is not required in this heating surface side.