Patents by Inventor Zifang Deng

Zifang Deng has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • BACTERIAL DNA GYRASE INHIBITORS AND METHODS OF USE THEREOF
    Publication number: 20230364057
    Abstract: The subject invention provides fluorophore-quencher nucleic acid molecules comprising relaxed or supercoiled DNA molecules, and their use in rapid and efficient high-throughput screening (HTS) assays to screen and identify compounds that inhibit DNA gyrases. These compounds can be used as antibiotics for treating bacterial infections, especially, multidrug resistant bacterial infections.
    Type: Application
    Filed: April 26, 2023
    Publication date: November 16, 2023
    Inventors: Fenfei LENG, Eddy ALFONSO, Zifang DENG, Adel NEFZI, Kyle ROHDE
  • T5 exonuclease-based method to identify DNA topoisomerase inhibitors
    Patent number: 11732286
    Abstract: The present invention provides assays and methods for studying DNA topology and topoisomerases. The assays and methods utilize a circular plasmid DNA comprising one or more hairpin structures and the ability of T5 exonuclease (T5E) to digest the circular plasmid DNA in a specific configuration. The assays and methods can be used as a high throughput screening for inhibitors of, for example, DNA gyrases and DNA topoisomerases I for anticancer drug and antibiotics discovery.
    Type: Grant
    Filed: February 14, 2022
    Date of Patent: August 22, 2023
    Assignee: The Florida International University Board of Trustees
    Inventors: Fenfei Leng, Zifang Deng
  • BACTERIAL DNA GYRASE INHIBITORS AND METHODS OF USE THEREOF
    Publication number: 20230136466
    Abstract: The subject invention provides fluorophore-quencher nucleic acid molecules comprising relaxed or supercoiled DNA molecules, and their use in rapid and efficient high-throughput screening (HTS) assays to screen and identify compounds that inhibit DNA gyrases. These compounds can be used as antibiotics for treating bacterial infections, especially, multidrug resistant bacterial infections.
    Type: Application
    Filed: September 13, 2022
    Publication date: May 4, 2023
    Applicant: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: FENFEI LENG, EDDY ALFONSO, ZIFANG DENG
  • T5 EXONUCLEASE-BASED METHOD TO IDENTIFY DNA TOPOISOMERASE INHIBITORS
    Publication number: 20220170070
    Abstract: The present invention provides assays and methods for studying DNA topology and topoisomerases. The assays and methods utilize a circular plasmid DNA comprising one or more hairpin structures and the ability of T5 exonuclease (T5E) to digest the circular plasmid DNA in a specific configuration. The assays and methods can be used as a high throughput screening for inhibitors of, for example, DNA gyrases and DNA topoisomerases I for anticancer drug and antibiotics discovery.
    Type: Application
    Filed: February 14, 2022
    Publication date: June 2, 2022
    Applicant: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Fenfei LENG, Zifang DENG
  • T5 EXONUCLEASE-BASED METHOD TO IDENTIFY DNA TOPOISOMERASE INHIBITORS
    Publication number: 20220119858
    Abstract: The present invention provides assays and methods for studying DNA topology and topoisomerases. The assays and methods utilize a circular plasmid DNA comprising one or more hairpin structures and the ability of T5 exonuclease (T5E) to digest the circular plasmid DNA in a specific configuration. The assays and methods can be used as a high throughput screening for inhibitors of, for example, DNA gyrases and DNA topoisomerases I for anticancer drug and antibiotics discovery.
    Type: Application
    Filed: October 16, 2020
    Publication date: April 21, 2022
    Applicant: The Florida International University Board of Trustees
    Inventors: Fenfei LENG, Zifang Deng
  • T5 exonuclease-based method to identify DNA topoisomerase inhibitors
    Patent number: 11286514
    Abstract: The present invention provides assays and methods for studying DNA topology and topoisomerases. The assays and methods utilize a circular plasmid DNA comprising one or more hairpin structures and the ability of T5 exonuclease (T5E) to digest the circular plasmid DNA in a specific configuration. The assays and methods can be used as a high throughput screening for inhibitors of, for example, DNA gyrases and DNA topoisomerases I for anticancer drug and antibiotics discovery.
    Type: Grant
    Filed: October 16, 2020
    Date of Patent: March 29, 2022
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Fenfei Leng, Zifang Deng
  • T5 exonuclease-based method to identify DNA topoisomerase inhibitors
    Patent number: 11268129
    Abstract: The present invention provides assays and methods for studying DNA topology and topoisomerases. The assays and methods utilize a circular plasmid DNA comprising one or more hairpin structures and the ability of T5 exonuclease (T5E) to digest the circular plasmid DNA in a specific configuration. The assays and methods can be used as a high throughput screening for inhibitors of, for example, DNA gyrases and DNA topoisomerases I for anticancer drug and antibiotics discovery.
    Type: Grant
    Filed: June 10, 2021
    Date of Patent: March 8, 2022
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Fenfei Leng, Zifang Deng