Abstract: A frame for constructing nerve tract is provided, including microcatheters, a support, and a shell. The shell is configured to contain a culture medium inside. The microcatheters are configured to culture nerve cells. The multiple microcatheters are suspended and fixed into the shell by the support, the microcatheters are arranged along a direction from one end to the other end of the shell, catheter walls of the microcatheters are provided with through holes, the nerve cells in the microcatheter cannot flow out through the through holes, and the culture medium enters the microcatheter through the through holes. A method for constructing nerve tract based on the frame described is provided, including: filling the nerve cells wrapped with a collagen hydrogel stock solution into the microcatheters, and after the collagen hydrogel stock solution is completely cross-linked, placing the frame loaded with the nerve cells in a culture device for perfusion culture.