Abstract: Provided herein are a device for well plate temperature control, a method of independently controlling the temperature in individual wells of a microwell plate, and a temperature-responsive protein. The device includes a microwell plate with at least one well formed therein; a temperature control assembly including a printed circuit board with at least one pair of thermistors extending therefrom, each of the at least one pair of thermistors arranged and disposed to align with one of the at least one wells; and a microcontroller configured to individually control each of the at least one pair of thermistors. The method includes positioning the temperature control assembly adjacent to the well plate such that each pair of thermistors extends into one of the wells, and independently providing a current flow to each of the at least one pair of thermistors to separate heat each well.